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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1ajj | ||||||
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タイトル | LDL RECEPTOR LIGAND-BINDING MODULE 5, CALCIUM-COORDINATING | ||||||
![]() | LOW-DENSITY LIPOPROTEIN RECEPTOR | ||||||
![]() | RECEPTOR / LDL RECEPTOR / CYSTEINE-RICH MODULE / CALCIUM | ||||||
機能・相同性 | ![]() regulation of phosphatidylcholine catabolic process / plasma lipoprotein particle clearance / positive regulation of lysosomal protein catabolic process / very-low-density lipoprotein particle receptor activity / negative regulation of astrocyte activation / receptor-mediated endocytosis involved in cholesterol transport / negative regulation of microglial cell activation / PCSK9-LDLR complex / cholesterol import / negative regulation of receptor recycling ...regulation of phosphatidylcholine catabolic process / plasma lipoprotein particle clearance / positive regulation of lysosomal protein catabolic process / very-low-density lipoprotein particle receptor activity / negative regulation of astrocyte activation / receptor-mediated endocytosis involved in cholesterol transport / negative regulation of microglial cell activation / PCSK9-LDLR complex / cholesterol import / negative regulation of receptor recycling / low-density lipoprotein particle clearance / clathrin heavy chain binding / low-density lipoprotein particle receptor activity / positive regulation of triglyceride biosynthetic process / negative regulation of low-density lipoprotein particle clearance / intestinal cholesterol absorption / low-density lipoprotein particle binding / Chylomicron clearance / response to caloric restriction / amyloid-beta clearance by cellular catabolic process / LDL clearance / regulation of protein metabolic process / high-density lipoprotein particle clearance / lipoprotein catabolic process / phospholipid transport / cholesterol transport / low-density lipoprotein particle / endolysosome membrane / negative regulation of amyloid fibril formation / negative regulation of protein metabolic process / artery morphogenesis / cellular response to fatty acid / regulation of cholesterol metabolic process / amyloid-beta clearance / sorting endosome / lipoprotein particle binding / cellular response to low-density lipoprotein particle stimulus / long-term memory / phagocytosis / Retinoid metabolism and transport / clathrin-coated pit / somatodendritic compartment / cholesterol metabolic process / receptor-mediated endocytosis / cholesterol homeostasis / clathrin-coated endocytic vesicle membrane / lipid metabolic process / positive regulation of inflammatory response / endocytosis / Cargo recognition for clathrin-mediated endocytosis / late endosome / apical part of cell / Clathrin-mediated endocytosis / virus receptor activity / amyloid-beta binding / basolateral plasma membrane / protease binding / molecular adaptor activity / lysosome / early endosome / receptor complex / endosome membrane / external side of plasma membrane / negative regulation of gene expression / calcium ion binding / positive regulation of gene expression / Golgi apparatus / cell surface / identical protein binding / membrane / plasma membrane 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Fass, D. / Blacklow, S.C. / Kim, P.S. / Berger, J.M. | ||||||
![]() | ![]() タイトル: Molecular basis of familial hypercholesterolaemia from structure of LDL receptor module. 著者: Fass, D. / Blacklow, S. / Kim, P.S. / Berger, J.M. #1: ![]() タイトル: Protein Folding and Calcium Binding Defects Arising from Familial Hypercholesterolemia Mutations of the Ldl Receptor 著者: Blacklow, S.C. / Kim, P.S. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 16.2 KB | 表示 | ![]() |
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PDB形式 | ![]() | 11.5 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 371.3 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 371.3 KB | 表示 | |
XML形式データ | ![]() | 2.3 KB | 表示 | |
CIF形式データ | ![]() | 3.1 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質・ペプチド | 分子量: 4069.431 Da / 分子数: 1 / 断片: LIGAND-BINDING DOMAIN, FIFTH REPEAT / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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#2: 化合物 | ChemComp-SO4 / |
#3: 化合物 | ChemComp-CA / |
#4: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 1.81 Å3/Da / 溶媒含有率: 28 % | |||||||||||||||||||||||||||||||||||
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結晶化 | pH: 5 詳細: PROTEIN WAS CRYSTALLIZED FROM 2.1 M AMMONIUM SULFATE, PH 5.0, 25% SUCROSE | |||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 温度: 15 ℃ / 手法: 蒸気拡散法, ハンギングドロップ法 | |||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 93 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: RIGAKU / 検出器: IMAGE PLATE / 日付: 1996年11月1日 / 詳細: MIRRORS |
放射 | モノクロメーター: NI FILTER / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 1.7→15 Å / Num. obs: 2959 / % possible obs: 94.3 % / Observed criterion σ(I): 1.5 / 冗長度: 4.5 % / Rsym value: 0.054 / Net I/σ(I): 20 |
反射 シェル | 解像度: 1.7→1.76 Å / 冗長度: 3 % / Mean I/σ(I) obs: 7 / Rsym value: 0.169 / % possible all: 87.7 |
反射 | *PLUS Rmerge(I) obs: 0.054 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]()
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精密化ステップ | サイクル: LAST / 解像度: 1.7→15 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS Rfactor Rfree: 0.24 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS |