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Yorodumi- PDB-1ahm: DER F 2, THE MAJOR MITE ALLERGEN FROM DERMATOPHAGOIDES FARINAE, N... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ahm | ||||||
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Title | DER F 2, THE MAJOR MITE ALLERGEN FROM DERMATOPHAGOIDES FARINAE, NMR, 10 STRUCTURES | ||||||
Components | DER F 2 | ||||||
Keywords | ALLERGEN / IMMUNOGLOBULIN FOLD | ||||||
Function / homology | Function and homology information intracellular cholesterol transport / cholesterol efflux / cholesterol binding / extracellular region Similarity search - Function | ||||||
Biological species | Dermatophagoides farinae (American house dust mite) | ||||||
Method | SOLUTION NMR / DYNAMICAL SIMULATED ANNEALING | ||||||
Authors | Ichikawa, S. / Hatanaka, H. / Yuuki, T. / Iwamoto, N. / Ogura, K. / Okumura, Y. / Inagaki, F. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1998 Title: Solution structure of Der f 2, the major mite allergen for atopic diseases. Authors: Ichikawa, S. / Hatanaka, H. / Yuuki, T. / Iwamoto, N. / Kojima, S. / Nishiyama, C. / Ogura, K. / Okumura, Y. / Inagaki, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ahm.cif.gz | 434.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ahm.ent.gz | 376.7 KB | Display | PDB format |
PDBx/mmJSON format | 1ahm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ahm_validation.pdf.gz | 355.4 KB | Display | wwPDB validaton report |
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Full document | 1ahm_full_validation.pdf.gz | 531.2 KB | Display | |
Data in XML | 1ahm_validation.xml.gz | 54.1 KB | Display | |
Data in CIF | 1ahm_validation.cif.gz | 73.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ah/1ahm ftp://data.pdbj.org/pub/pdb/validation_reports/ah/1ahm | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14064.193 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Dermatophagoides farinae (American house dust mite) Cellular location: EXTRACELLULAR / Plasmid: PFLT1 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q00855 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 5.6 / Temperature: 328 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITYPLUS 600 / Manufacturer: Varian / Model: UNITYPLUS 600 / Field strength: 600 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: DYNAMICAL SIMULATED ANNEALING / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||
NMR ensemble | Conformer selection criteria: LEAST FNOE+FREPEL / Conformers calculated total number: 20 / Conformers submitted total number: 10 |