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Yorodumi- PDB-1aft: SMALL SUBUNIT C-TERMINAL INHIBITORY PEPTIDE OF MOUSE RIBONUCLEOTI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1aft | ||||||
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Title | SMALL SUBUNIT C-TERMINAL INHIBITORY PEPTIDE OF MOUSE RIBONUCLEOTIDE REDUCTASE AS BOUND TO THE LARGE SUBUNIT, NMR, 26 STRUCTURES | ||||||
Components | RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE | ||||||
Keywords | RIBONUCLEOTIDE REDUCTASE / PEPTIDE INHIBITORS / TRANSFERRED NOESY / RESTRAINED MOLECULAR DYNAMICS / NONSTANDARD TYPE I TURN | ||||||
Function / homology | Function and homology information deoxyribonucleotide metabolic process / Interconversion of nucleotide di- and triphosphates / ribonucleoside diphosphate metabolic process / 2'-deoxyribonucleotide biosynthetic process / ribonucleoside-diphosphate reductase complex / ribonucleoside-diphosphate reductase / ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor / deoxyribonucleotide biosynthetic process / protein heterotetramerization / positive regulation of G1/S transition of mitotic cell cycle ...deoxyribonucleotide metabolic process / Interconversion of nucleotide di- and triphosphates / ribonucleoside diphosphate metabolic process / 2'-deoxyribonucleotide biosynthetic process / ribonucleoside-diphosphate reductase complex / ribonucleoside-diphosphate reductase / ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor / deoxyribonucleotide biosynthetic process / protein heterotetramerization / positive regulation of G1/S transition of mitotic cell cycle / blastocyst development / ferric iron binding / nuclear envelope / protein homodimerization activity / identical protein binding / cytosol Similarity search - Function | ||||||
Method | SOLUTION NMR / SIMULATED ANNEALING IMPLEMENTED WITH RESTRAINED MOLECULAR DYNAMICS | ||||||
Authors | Laub, P.B. / Fisher, A.L. / Furst, G.T. / Barwis, B.A. / Hamann, C.S. / Cooperman, B.S. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1995 Title: NMR structure of an inhibitory R2 C-terminal peptide bound to mouse ribonucleotide reductase R1 subunit. Authors: Fisher, A. / Laub, P.B. / Cooperman, B.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1aft.cif.gz | 51.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1aft.ent.gz | 38.9 KB | Display | PDB format |
PDBx/mmJSON format | 1aft.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1aft_validation.pdf.gz | 338 KB | Display | wwPDB validaton report |
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Full document | 1aft_full_validation.pdf.gz | 474.2 KB | Display | |
Data in XML | 1aft_validation.xml.gz | 5.2 KB | Display | |
Data in CIF | 1aft_validation.cif.gz | 8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/af/1aft ftp://data.pdbj.org/pub/pdb/validation_reports/af/1aft | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 853.916 Da / Num. of mol.: 1 / Fragment: C-TERMINAL RESIDUES AC-FTLDADF OF SMALL SUBUNIT Source method: isolated from a genetically manipulated source Details: SYNTHESIZED USING FMOC CHEMISTRY References: UniProt: P11157, ribonucleoside-diphosphate reductase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 7 / Temperature: 287 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: SIMULATED ANNEALING IMPLEMENTED WITH RESTRAINED MOLECULAR DYNAMICS Software ordinal: 1 Details: SEE JRNL REFERENCE FOR REFINEMENT DETAILS. THE AUTHORS DID NOT MAKE OR ATTEMPT TO MAKE STEREOSPECIFIC ASSIGNMENTS. | ||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATIONS, POTENTIAL ENERGY Conformers calculated total number: 299 / Conformers submitted total number: 26 |