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Open data
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Basic information
Entry | Database: PDB / ID: 1ady | ||||||
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Title | HISTIDYL-TRNA SYNTHETASE IN COMPLEX WITH HISTIDYL-ADENYLATE | ||||||
![]() | HISTIDYL-TRNA SYNTHETASE | ||||||
![]() | TRNA SYNTHETASE / HISTIDYL-ADENYLATE / AMINO ACID | ||||||
Function / homology | ![]() histidine-tRNA ligase / histidine-tRNA ligase activity / histidyl-tRNA aminoacylation / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cusack, S. / Aberg, A. | ||||||
![]() | ![]() Title: Crystal structure analysis of the activation of histidine by Thermus thermophilus histidyl-tRNA synthetase. Authors: Aberg, A. / Yaremchuk, A. / Tukalo, M. / Rasmussen, B. / Cusack, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 327.7 KB | Display | ![]() |
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PDB format | ![]() | 269.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 602.7 KB | Display | ![]() |
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Full document | ![]() | 631.1 KB | Display | |
Data in XML | ![]() | 36.1 KB | Display | |
Data in CIF | ![]() | 53.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 47125.918 Da / Num. of mol.: 4 / Source method: isolated from a natural source Details: HISTIDYL-ADENYLATE PRODUCED ENZYMATICALLY IN THE CRYSTAL IS PRESENT IN THE ACTIVE SITE Source: (natural) ![]() ![]() #2: Chemical | ChemComp-SO4 / #3: Chemical | ChemComp-HAM / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.83 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7. | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 31, 1995 / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→30 Å / Num. obs: 32840 / % possible obs: 65 % / Redundancy: 2.6 % / Rmerge(I) obs: 0.1 / Rsym value: 0.1 / Net I/σ(I): 5 |
Reflection | *PLUS Num. measured all: 85481 |
Reflection shell | *PLUS % possible obs: 30 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: HISTIDYL-TRNA SYNTHETASE Resolution: 2.8→20 Å
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Refinement step | Cycle: LAST / Resolution: 2.8→20 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Num. reflection Rfree: 1632 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |