+Open data
-Basic information
Entry | Database: PDB / ID: 1a7j | ||||||
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Title | PHOSPHORIBULOKINASE FROM RHODOBACTER SPHEROIDES | ||||||
Components | PHOSPHORIBULOKINASE | ||||||
Keywords | TRANSFERASE / KINASE / CALVIN CYCLE | ||||||
Function / homology | Function and homology information phosphoribulokinase / phosphoribulokinase activity / reductive pentose-phosphate cycle / ATP binding Similarity search - Function | ||||||
Biological species | Rhodobacter sphaeroides (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MIR / Resolution: 2.5 Å | ||||||
Authors | Harrison, D.H.T. / Runquist, J. / Holub, A. / Miziorko, H. | ||||||
Citation | Journal: Biochemistry / Year: 1998 Title: The crystal structure of phosphoribulokinase from Rhodobacter sphaeroides reveals a fold similar to that of adenylate kinase. Authors: Harrison, D.H. / Runquist, J.A. / Holub, A. / Miziorko, H.M. #1: Journal: Biochemistry / Year: 1998 Title: Functional Evaluation of Invariant Arginines Situated in the Mobile Lid Domain of Phosphoribulokinase Authors: Runquist, J.A. / Harrison, D.H. / Miziorko, H.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a7j.cif.gz | 67.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a7j.ent.gz | 50.6 KB | Display | PDB format |
PDBx/mmJSON format | 1a7j.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a7/1a7j ftp://data.pdbj.org/pub/pdb/validation_reports/a7/1a7j | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 32709.971 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rhodobacter sphaeroides (bacteria) / Cell line: BL21 / Gene: PRKA / Plasmid: PETBPRKWT / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P12033, phosphoribulokinase |
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#2: Chemical | ChemComp-SO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.86 % | |||||||||||||||||||||||||
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Crystal grow | pH: 5 Details: PROTEIN WAS CRYSTALLIZED FROM AMMONIUM PHOSPHATE, pH 5.0 | |||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8.2 / Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH2R / Wavelength: 1.5418 |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Aug 1, 1996 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→30 Å / Num. obs: 10956 / % possible obs: 80.6 % / Observed criterion σ(I): 2 / Redundancy: 4 % / Rmerge(I) obs: 0.062 |
Reflection | *PLUS Num. measured all: 42132 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2.5→15 Å / Data cutoff high absF: 1000000 / Data cutoff low absF: 0.001 / Cross valid method: THROUGHOUT / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 2.5→15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.61 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.298 |