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- PDB-1a68: CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POT... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1a68 | ||||||
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Title | CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL | ||||||
![]() | POTASSIUM CHANNEL KV1.1 | ||||||
![]() | POTASSIUM CHANNELS / TETRAMERIZATION DOMAIN / APLYSIA KV1.1 | ||||||
Function / homology | ![]() delayed rectifier potassium channel activity / voltage-gated potassium channel complex / protein homooligomerization Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kreusch, A. / Pfaffinger, P.J. / Stevens, C.F. / Choe, S. | ||||||
![]() | ![]() Title: Crystal structure of the tetramerization domain of the Shaker potassium channel. Authors: Kreusch, A. / Pfaffinger, P.J. / Stevens, C.F. / Choe, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 29.9 KB | Display | ![]() |
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PDB format | ![]() | 19.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 363.5 KB | Display | ![]() |
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Full document | ![]() | 365.3 KB | Display | |
Data in XML | ![]() | 3.1 KB | Display | |
Data in CIF | ![]() | 4.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11493.914 Da / Num. of mol.: 1 / Fragment: TETRAMERIZATION DOMAIN / Mutation: INSERTED MET AT N-TERMINUS Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Tissue: CENTRAL NERVOUS SYSTEM / Cell line: BL21 / Cellular location: CYTOPLASM / Plasmid: PET20B / Species (production host): Escherichia coli / Cellular location (production host): CYTOPLASM / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 39 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.5 Details: PROTEIN WAS CRYSTALLIZED FROM 7-8% PEG8000, 300 MM NACL, 200MM MGCL2, 20 MM TRIS, PH 8.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 15 ℃ / Method: vapor diffusion | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 290 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: SIEMENS / Detector: CCD AREA DETECTOR / Date: Aug 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9799 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→25 Å / Num. obs: 7712 / % possible obs: 99 % / Observed criterion σ(I): 3 / Redundancy: 10.9 % / Biso Wilson estimate: 18.9 Å2 / Rsym value: 0.063 / Net I/σ(I): 8.8 |
Reflection shell | Resolution: 1.8→1.91 Å / Mean I/σ(I) obs: 3.5 / Rsym value: 0.035 / % possible all: 97.2 |
Reflection | *PLUS Rmerge(I) obs: 0.063 |
Reflection shell | *PLUS % possible obs: 97.2 % / Rmerge(I) obs: 0.035 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 26.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.8→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.91 Å / Rfactor Rfree error: 0.038 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.334 |