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Yorodumi- PDB-1a5j: CHICKEN B-MYB DNA BINDING DOMAIN, REPEAT 2 AND REPEAT3, NMR, 32 S... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1a5j | ||||||
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Title | CHICKEN B-MYB DNA BINDING DOMAIN, REPEAT 2 AND REPEAT3, NMR, 32 STRUCTURES | ||||||
Components | B-MYB | ||||||
Keywords | DNA BINDING PROTEIN / DNA-BINDING PROTEIN / PROTOONCOGENE PRODUCT | ||||||
Function / homology | Function and homology information transcription coregulator activity / mitotic cell cycle / DNA-binding transcription activator activity, RNA polymerase II-specific / sequence-specific DNA binding / transcription cis-regulatory region binding / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / nucleus Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Mcintosh, P.B. / Carr, M.D. / Wollborn, U. / Frenkiel, T.A. / Feeney, J. / Mccormick, J.E. / Klempnauer, K.H. | ||||||
Citation | Journal: Biochemistry / Year: 1998 Title: Solution structure of the B-Myb DNA-binding domain: a possible role for conformational instability of the protein in DNA binding and control of gene expression. Authors: McIntosh, P.B. / Frenkiel, T.A. / Wollborn, U. / McCormick, J.E. / Klempnauer, K.H. / Feeney, J. / Carr, M.D. #1: Journal: Eur.J.Biochem. / Year: 1996 Title: Structure of the B-Myb DNA-Binding Domain in Solution and Evidence for Multiple Conformations in the Region of Repeat-2 Involved in DNA Binding. Implications for Sequence-Specific DNA Binding by Myb Proteins Authors: Carr, M.D. / Wollborn, U. / Mcintosh, P.B. / Frenkiel, T.A. / Mccormick, J.E. / Bauer, C.J. / Klempnauer, K.H. / Feeney, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a5j.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1a5j.ent.gz | 959 KB | Display | PDB format |
PDBx/mmJSON format | 1a5j.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1a5j_validation.pdf.gz | 346.1 KB | Display | wwPDB validaton report |
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Full document | 1a5j_full_validation.pdf.gz | 663 KB | Display | |
Data in XML | 1a5j_validation.xml.gz | 112.8 KB | Display | |
Data in CIF | 1a5j_validation.cif.gz | 140.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a5/1a5j ftp://data.pdbj.org/pub/pdb/validation_reports/a5/1a5j | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12958.922 Da / Num. of mol.: 1 / Fragment: DNA BINDING DOMAIN, REPEAT 2 AND REPEAT 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Plasmid: PGEX-3X / Production host: Escherichia coli (E. coli) / Strain (production host): HB101 / References: UniProt: Q03237 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURES WERE DETERMINED USING DOUBLE AND TRIPLE RESONANCE NMR SPECTROSCOPY ON 13C/15N AND 15N LABELED B-MYB. |
-Sample preparation
Sample conditions | pH: 6.0 / Temperature: 288 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||
NMR ensemble | Conformer selection criteria: NO DISTANCE CONSTRAINT ORVAN DER WAALS VIOLATIONS >0.5A,NO DIHEDRAL ANGLE CONSTRAINT VIOLATIONS >6 DEGREES Conformers calculated total number: 100 / Conformers submitted total number: 32 |