- EMDB-8924: Structure of the Salmonella SPI-1 type III secretion injectisome ... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-8924
タイトル
Structure of the Salmonella SPI-1 type III secretion injectisome needle filament
マップデータ
Cryo-EM reconstruction of the Salmonella SPI-1 type III secretion injectisome needle filament
試料
複合体: PrgI helical filament
タンパク質・ペプチド: Protein PrgI
キーワード
Type III secretion system / MEMBRANE PROTEIN
機能・相同性
機能・相同性情報
type III protein secretion system complex / protein secretion by the type III secretion system / cell surface / extracellular region / identical protein binding 類似検索 - 分子機能
Type III secretion, needle-protein-like / Type III secretion, needle-protein-like superfamily / Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF / Type III secretion system, needle protein 類似検索 - ドメイン・相同性
SPI-1 type 3 secretion system needle filament protein 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2018 タイトル: Cryo-EM analysis of the T3S injectisome reveals the structure of the needle and open secretin. 著者: J Hu / L J Worrall / C Hong / M Vuckovic / C E Atkinson / N Caveney / Z Yu / N C J Strynadka / 要旨: The bacterial type III secretion system, or injectisome, is a syringe shaped nanomachine essential for the virulence of many disease causing Gram-negative bacteria. At the core of the injectisome ...The bacterial type III secretion system, or injectisome, is a syringe shaped nanomachine essential for the virulence of many disease causing Gram-negative bacteria. At the core of the injectisome structure is the needle complex, a continuous channel formed by the highly oligomerized inner and outer membrane hollow rings and a polymerized helical needle filament which spans through and projects into the infected host cell. Here we present the near-atomic resolution structure of a needle complex from the prototypical Salmonella Typhimurium SPI-1 type III secretion system, with local masking protocols allowing for model building and refinement of the major membrane spanning components of the needle complex base in addition to an isolated needle filament. This work provides significant insight into injectisome structure and assembly and importantly captures the molecular basis for substrate induced gating in the giant outer membrane secretin portal family.