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- EMDB-8844: Influenza A virus-like particles containing Hemagglutinin, Neuram... -

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Basic information

Entry
Database: EMDB / ID: 8844
TitleInfluenza A virus-like particles containing Hemagglutinin, Neuraminidase, M1, and M2 proteins
SampleInfluenza A virus
SourceInfluenza A virus / virus
Map dataInfluenza A virus-like particles containing Hemagglutinin, Neuraminidase, M1, and M2 proteins
Methodelectron tomography
AuthorsChlanda P / Zimmerberg J
CitationJ. Virol., 2017

J. Virol., 2017 StrPapers
Palmitoylation contributes to membrane curvature in Influenza A virus assembly and hemagglutinin-mediated membrane fusion.
Petr Chlanda / Elena Mekhedov / Hang Waters / Alexander Sodt / Cindi Schwartz / Vinod Nair / Paul S Blank / Joshua Zimmerberg

DateDeposition: Jul 24, 2017 / Header (metadata) release: Aug 9, 2017 / Map release: Aug 9, 2017 / Last update: Aug 9, 2017

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Map

Fileemd_8844.map.gz (map file in CCP4 format, 310222 KB)
Voxel sizeX=Y=Z: 7.367 Å
Density
Contour Level:1 (by author)
Minimum - Maximum-128 - 127
Average (Standard dev.)49.57716 (8.193042)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions13041220195
Origin000
Limit13031219194
Spacing12201304195
CellA: 8987.74 Å / B: 9606.568 Å / C: 1436.5651 Å
α=β=γ: 90 deg.

CCP4 map header:

modeenvelope stored as signed bytes (from -128 lowest to 127 highest)
Å/pix. X/Y/Z7.3677.3677.367
M x/y/z12201304195
origin x/y/z0.0000.0000.000
length x/y/z8987.7409606.5681436.565
α/β/γ90.00090.00090.000
start NX/NY/NZ-34-26-36
NX/NY/NZ528549
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS12201304195
D min/max/mean-128.000127.00049.577

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Supplemental data

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Sample components

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Entire Influenza A virus

EntireName: Influenza A virus
Details: Influenza A virus-like particles containing HA, NA, M1, and M2 proteins
Number of components: 1

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Component #1: virus, Influenza A virus

VirusName: Influenza A virus / Class: VIRUS-LIKE PARTICLE
Details: Influenza A virus-like particles containing HA, NA, M1, and M2 proteins
Empty: Yes / Enveloped: Yes / Isolate: OTHER
SpeciesSpecies: Influenza A virus / virus

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Experimental details

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Sample preparation

Specimen stateparticle
Sample solutionpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 1.3 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: FEI FALCON II (4k x 4k)

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Image processing

ProcessingMethod: electron tomography / Number of sections: 61
3D reconstructionAlgorithm: BACK PROJECTION / Software: Imod

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