+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-8844 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
タイトル | Influenza A virus-like particles containing Hemagglutinin, Neuraminidase, M1, and M2 proteins | |||||||||
マップデータ | Influenza A virus-like particles containing Hemagglutinin, Neuraminidase, M1, and M2 proteins | |||||||||
試料 | Influenza virus != Influenza A virus Influenza virus
| |||||||||
生物種 | Influenza A virus (A型インフルエンザウイルス) | |||||||||
手法 | 電子線トモグラフィー法 / クライオ電子顕微鏡法 | |||||||||
データ登録者 | Chlanda P / Zimmerberg J | |||||||||
引用 | ジャーナル: J Virol / 年: 2017 タイトル: Palmitoylation Contributes to Membrane Curvature in Influenza A Virus Assembly and Hemagglutinin-Mediated Membrane Fusion. 著者: Petr Chlanda / Elena Mekhedov / Hang Waters / Alexander Sodt / Cindi Schwartz / Vinod Nair / Paul S Blank / Joshua Zimmerberg / 要旨: The highly conserved cytoplasmic tail of influenza virus glycoprotein hemagglutinin (HA) contains three cysteines, posttranslationally modified by covalently bound fatty acids. While viral HA ...The highly conserved cytoplasmic tail of influenza virus glycoprotein hemagglutinin (HA) contains three cysteines, posttranslationally modified by covalently bound fatty acids. While viral HA acylation is crucial in virus replication, its physico-chemical role is unknown. We used virus-like particles (VLP) to study the effect of acylation on morphology, protein incorporation, lipid composition, and membrane fusion. Deacylation interrupted HA-M1 interactions since deacylated mutant HA failed to incorporate an M1 layer within spheroidal VLP, and filamentous particles incorporated increased numbers of neuraminidase (NA). While HA acylation did not influence VLP shape, lipid composition, or HA lateral spacing, acylation significantly affected envelope curvature. Compared to wild-type HA, deacylated HA is correlated with released particles with flat envelope curvature in the absence of the matrix (M1) protein layer. The spontaneous curvature of palmitate was calculated by molecular dynamic simulations and was found to be comparable to the curvature values derived from VLP size distributions. Cell-cell fusion assays show a strain-independent failure of fusion pore enlargement among H2 (A/Japan/305/57), H3 (A/Aichi/2/68), and H3 (A/Udorn/72) viruses. In contradistinction, acylation made no difference in the low-pH-dependent fusion of isolated VLPs to liposomes: fusion pores formed and expanded, as demonstrated by the presence of complete fusion products observed using cryo-electron tomography (cryo-ET). We propose that the primary mechanism of action of acylation is to control membrane curvature and to modify HA's interaction with M1 protein, while the stunting of fusion by deacylated HA acting in isolation may be balanced by other viral proteins which help lower the energetic barrier to pore expansion. Influenza A virus is an airborne pathogen causing seasonal epidemics and occasional pandemics. Hemagglutinin (HA), a glycoprotein abundant on the virion surface, is important in both influenza A virus assembly and entry. HA is modified by acylation whose removal abrogates viral replication. Here, we used cryo-electron tomography to obtain three-dimensional images to elucidate a role for HA acylation in VLP assembly. Our data indicate that HA acylation contributes to the capability of HA to bend membranes and to HA's interaction with the M1 scaffold protein during virus assembly. Furthermore, our data on VLP and, by hypothesis, virus suggests that HA acylation, while not critical to fusion pore formation, contributes to pore expansion in a target-dependent fashion. | |||||||||
履歴 |
|
-構造の表示
ムービー |
ムービービューア |
---|---|
構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_8844.map.gz | 198.8 MB | EMDBマップデータ形式 | |
---|---|---|---|---|
ヘッダ (付随情報) | emd-8844-v30.xml emd-8844.xml | 8.3 KB 8.3 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_8844.png | 154.3 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-8844 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-8844 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
---|
-マップ
ファイル | ダウンロード / ファイル: emd_8844.map.gz / 形式: CCP4 / 大きさ: 295.9 MB / タイプ: IMAGE STORED AS SIGNED BYTE | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
注釈 | Influenza A virus-like particles containing Hemagglutinin, Neuraminidase, M1, and M2 proteins | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 7.367 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
|
-添付データ
-試料の構成要素
-全体 : Influenza virus
全体 | 名称: Influenza virus (インフルエンザウイルス) |
---|---|
要素 |
|
-超分子 #1: Influenza A virus
超分子 | 名称: Influenza A virus / タイプ: virus / ID: 1 / 親要素: 0 詳細: Influenza A virus-like particles containing HA, NA, M1, and M2 proteins NCBI-ID: 11320 / 生物種: Influenza A virus / ウイルスタイプ: VIRUS-LIKE PARTICLE / ウイルス・単離状態: OTHER / ウイルス・エンベロープ: Yes / ウイルス・中空状態: Yes |
---|
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
解析 | 電子線トモグラフィー法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7 |
---|---|
凍結 | 凍結剤: ETHANE |
切片作成 | その他: NO SECTIONING |
位置合わせマーカー | Manufacturer: EMS / 直径: 10 nm |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy |
撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 1.3 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
最終 再構成 | アルゴリズム: BACK PROJECTION / ソフトウェア - 名称: IMOD / 使用した粒子像数: 61 |
---|