- EMDB-8838: Sub-tomogram average of I1 dynein Pre class -
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Basic information
Entry
Database: EMDB / ID: EMD-8838
Title
Sub-tomogram average of I1 dynein Pre class
Map data
Sub-tomogram average of I1 dynein Pre class: the two dynein heavy chains of sea urchin sperm flagellar I1 dynein complex are in the pre-powerstroke state.
Sample
Cell: Sea urchin sperm
Biological species
Strongylocentrotus purpuratus (purple sea urchin)
Method
subtomogram averaging / cryo EM / Resolution: 31.0 Å
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM083122
United States
March of Dimes Foundation
United States
Citation
Journal: Science / Year: 2018 Title: Asymmetric distribution and spatial switching of dynein activity generates ciliary motility. Authors: Jianfeng Lin / Daniela Nicastro / Abstract: Motile cilia and flagella are essential, highly conserved organelles, and their motility is driven by the coordinated activities of multiple dynein isoforms. The prevailing "switch-point" hypothesis ...Motile cilia and flagella are essential, highly conserved organelles, and their motility is driven by the coordinated activities of multiple dynein isoforms. The prevailing "switch-point" hypothesis posits that dyneins are asymmetrically activated to drive flagellar bending. To test this model, we applied cryo-electron tomography to visualize activity states of individual dyneins relative to their locations along beating flagella of sea urchin sperm cells. As predicted, bending was generated by the asymmetric distribution of dynein activity on opposite sides of the flagellum. However, contrary to predictions, most dyneins were in their active state, and the smaller population of conformationally inactive dyneins switched flagellar sides relative to the bending direction. Thus, our data suggest a "switch-inhibition" mechanism in which force imbalance is generated by inhibiting, rather than activating, dyneins on alternating sides of the flagellum.
History
Deposition
Jul 19, 2017
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Header (metadata) release
Aug 23, 2017
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Map release
May 9, 2018
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Update
Jan 29, 2020
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Current status
Jan 29, 2020
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
EMPIAR-10157 (Title: Cryo electron tomography of sea urchin sperm flagella Data size: 15.8 Data #1: Tilt series of sea urchin sperm flagella that were frozen while they were actively beating. [tilt series])
Download / File: emd_8838.map.gz / Format: CCP4 / Size: 704.1 KB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation
Sub-tomogram average of I1 dynein Pre class: the two dynein heavy chains of sea urchin sperm flagellar I1 dynein complex are in the pre-powerstroke state.
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