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- EMDB-8705: Negative stain electron microscopy map of human CD22 extracellula... -

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Basic information

Entry
Database: EMDB / ID: 8705
TitleNegative stain electron microscopy map of human CD22 extracellular domain in complex with epratuzumab Fab
Samplehuman CD22 B-cell receptor
SourceHomo sapiens / human
Map dataNegative stain electron microscopy map of CD22 extracellular domain in complex with Fab fragment of epratuzumab
Methodsingle particle reconstruction, at 30 Å resolution
AuthorsJulien JP / Ereno-Orbea J / Sicard T
CitationNat Commun, 2017, 8, 764-764

Nat Commun, 2017, 8, 764-764 Yorodumi Papers
Molecular basis of human CD22 function and therapeutic targeting.
June Ereño-Orbea / Taylor Sicard / Hong Cui / Mohammad T Mazhab-Jafari / Samir Benlekbir / Alba Guarné / John L Rubinstein / Jean-Philippe Julien

DateDeposition: Apr 24, 2017 / Header (metadata) release: Jun 14, 2017 / Last update: Jun 14, 2017

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Structure visualization

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Map

FileOn hold (released when the article is published)
Voxel sizeX=Y=Z: 2.7 Å
Density
Contour Level:4.79 (by author)
Minimum - Maximum0 - 15.929847
Average (Standard dev.)0.1983934 (0.70661753)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions646464
Origin000
Limit636363
Spacing646464
CellA=B=C: 172.8 Å
α=β=γ: 90 deg.

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Supplemental data

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Sample components

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Entire human CD22 B-cell receptor

EntireName: human CD22 B-cell receptor / Number of components: 1
MassExperimental: 90 kDa

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Component #1: cellular-component, human CD22 B-cell receptor

Cellular-componentName: human CD22 B-cell receptor / Recombinant expression: No
MassExperimental: 90 kDa
SourceSpecies: Homo sapiens / human
Source (engineered)Expression System: Homo sapiens / human

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Experimental details

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Sample preparation

Specimen stateparticle
Sample solutionSpecimen conc.: 0.03 mg/ml / pH: 9
VitrificationCryogen name: NONE

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Electron microscopy imaging

Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company
ImagingMicroscope: FEI TECNAI F20
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 30 e/Å2 / Illumination mode: OTHER
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN ORIUS SC200 (2k x 2k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 6312
3D reconstructionResolution: 30 Å / Resolution method: OTHER

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