+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-8691 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | IMPDH Filament in the expanded conformation | |||||||||
Map data | IMPDH expanded conformation | |||||||||
Sample |
| |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | helical reconstruction / negative staining / Resolution: 25.0 Å | |||||||||
Authors | Kollman JM / Burrell AL / Johnson MC | |||||||||
Citation | Journal: Mol Biol Cell / Year: 2017 Title: Reconstituted IMPDH polymers accommodate both catalytically active and inactive conformations. Authors: Sajitha A Anthony / Anika L Burrell / Matthew C Johnson / Krisna C Duong-Ly / Yin-Ming Kuo / Jacqueline C Simonet / Peter Michener / Andrew Andrews / Justin M Kollman / Jeffrey R Peterson / Abstract: Several metabolic enzymes undergo reversible polymerization into macromolecular assemblies. The function of these assemblies is often unclear but in some cases they regulate enzyme activity and ...Several metabolic enzymes undergo reversible polymerization into macromolecular assemblies. The function of these assemblies is often unclear but in some cases they regulate enzyme activity and metabolic homeostasis. The guanine nucleotide biosynthetic enzyme inosine monophosphate dehydrogenase (IMPDH) forms octamers that polymerize into helical chains. In mammalian cells, IMPDH filaments can associate into micron-length assemblies. Polymerization and enzyme activity are regulated in part by binding of purine nucleotides to an allosteric regulatory domain. ATP promotes octamer polymerization, whereas GTP promotes a compact, inactive conformation whose ability to polymerize is unknown. Also unclear is whether polymerization directly alters IMPDH catalytic activity. To address this, we identified point mutants of human IMPDH2 that either prevent or promote polymerization. Unexpectedly, we found that polymerized and non-assembled forms of recombinant IMPDH have comparable catalytic activity, substrate affinity, and GTP sensitivity and validated this finding in cells. Electron microscopy revealed that substrates and allosteric nucleotides shift the equilibrium between active and inactive conformations in both the octamer and the filament. Unlike other metabolic filaments, which selectively stabilize active or inactive conformations, recombinant IMPDH filaments accommodate multiple states. These conformational states are finely tuned by substrate availability and purine balance, while polymerization may allow cooperative transitions between states. | |||||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_8691.map.gz | 7.2 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-8691-v30.xml emd-8691.xml | 10.4 KB 10.4 KB | Display Display | EMDB header |
Images | emd_8691.png | 64.2 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-8691 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-8691 | HTTPS FTP |
-Validation report
Summary document | emd_8691_validation.pdf.gz | 79.2 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_8691_full_validation.pdf.gz | 78.3 KB | Display | |
Data in XML | emd_8691_validation.xml.gz | 495 B | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-8691 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-8691 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|
-Map
File | Download / File: emd_8691.map.gz / Format: CCP4 / Size: 8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | IMPDH expanded conformation | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4.14 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Sample components
-Entire : IMPDH filament, expanded conformation
Entire | Name: IMPDH filament, expanded conformation |
---|---|
Components |
|
-Supramolecule #1: IMPDH filament, expanded conformation
Supramolecule | Name: IMPDH filament, expanded conformation / type: complex / ID: 1 / Parent: 0 / Details: IMPDH assembled in the presence of ATP |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Escherichia coli BL21(DE3) (bacteria) / Recombinant strain: BL21(DE3) |
Molecular weight | Theoretical: 385 kDa/nm |
-Experimental details
-Structure determination
Method | negative staining |
---|---|
Processing | helical reconstruction |
Aggregation state | helical array |
-Sample preparation
Buffer | pH: 7.6 |
---|---|
Staining | Type: NEGATIVE / Material: uranyl formate |
Grid | Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE |
-Electron microscopy
Microscope | FEI TECNAI SPIRIT |
---|---|
Image recording | Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Average exposure time: 0.5 sec. / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 120 kV / Electron source: LAB6 |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Tecnai Spirit / Image courtesy: FEI Company |