- EMDB-8425: Chikungunya virus-like particle inserted with circumsporozoite pr... -
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データベース: EMDB / ID: EMD-8425
タイトル
Chikungunya virus-like particle inserted with circumsporozoite protein peptides of Plasmodium falciparum in complex with Fab fragment of neutralizing antibody DG2
マップデータ
Chikungunya virus-like particle inserted with circumsporozoite protein peptides of Plasmodium falciparum in complex with Fab fragment of neutralizing antibody DG2
ジャーナル: Clin Vaccine Immunol / 年: 2017 タイトル: Development of a Novel Virus-Like Particle Vaccine Platform That Mimics the Immature Form of Alphavirus. 著者: Akane Urakami / Atsuko Sakurai / Momoko Ishikawa / Moh Lan Yap / Yevel Flores-Garcia / Yasunari Haseda / Taiki Aoshi / Fidel P Zavala / Michael G Rossmann / Sachiko Kuno / Ryuji Ueno / Wataru Akahata / 要旨: Virus-like particles (VLPs) are noninfectious multiprotein structures that are engineered to self-assemble from viral structural proteins. Here, we developed a novel VLP-based vaccine platform ...Virus-like particles (VLPs) are noninfectious multiprotein structures that are engineered to self-assemble from viral structural proteins. Here, we developed a novel VLP-based vaccine platform utilizing VLPs from the chikungunya virus. We identified two regions within the envelope protein, a structural component of chikungunya, where foreign antigens can be inserted without compromising VLP structure. Our VLP displays 480 copious copies of an inserted antigen on the VLP surface in a highly symmetric manner and is thus capable of inducing strong immune responses against any inserted antigen. Furthermore, by mimicking the structure of the immature form of the virus, we altered our VLP's dynamics and enhanced its immunogenicity. We used the circumsporozoite protein (CSP) of the malaria parasite as an antigen and demonstrated that our VLP-based vaccine elicits strong immune responses against CSP in animals. The sera from immunized monkeys protected mice from malaria infection. Likewise, mice vaccinated with CSP-containing VLPs were protected from an infectious sporozoite challenge. Hence, our uniquely engineered VLP platform can serve as a blueprint for the development of vaccines against other pathogens and diseases.
ダウンロード / ファイル: emd_8425.map.gz / 形式: CCP4 / 大きさ: 216 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Chikungunya virus-like particle inserted with circumsporozoite protein peptides of Plasmodium falciparum in complex with Fab fragment of neutralizing antibody DG2