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Yorodumi- EMDB-7798: Epstein-Barr Virus glycoproteins, gH/gL/gp42 inserted ferritin na... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-7798 | |||||||||
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Title | Epstein-Barr Virus glycoproteins, gH/gL/gp42 inserted ferritin nanoparticles | |||||||||
Map data | primary map | |||||||||
Sample |
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Biological species | Human herpesvirus 4 (Epstein-Barr virus) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 23.0 Å | |||||||||
Authors | Rossmann MG / Cohen JI / Bu W / Yap ML | |||||||||
Citation | Journal: Immunity / Year: 2019 Title: Immunization with Components of the Viral Fusion Apparatus Elicits Antibodies That Neutralize Epstein-Barr Virus in B Cells and Epithelial Cells. Authors: Wei Bu / M Gordon Joyce / Hanh Nguyen / Dalton V Banh / Fiona Aguilar / Zeshan Tariq / Moh Lan Yap / Yusuke Tsujimura / Rebecca A Gillespie / Yaroslav Tsybovsky / Sarah F Andrews / Sandeep R ...Authors: Wei Bu / M Gordon Joyce / Hanh Nguyen / Dalton V Banh / Fiona Aguilar / Zeshan Tariq / Moh Lan Yap / Yusuke Tsujimura / Rebecca A Gillespie / Yaroslav Tsybovsky / Sarah F Andrews / Sandeep R Narpala / Adrian B McDermott / Michael G Rossmann / Yasuhiro Yasutomi / Gary J Nabel / Masaru Kanekiyo / Jeffrey I Cohen / Abstract: Epstein-Barr virus (EBV) causes infectious mononucleosis and is associated with epithelial-cell cancers and B cell lymphomas. An effective EBV vaccine is not available. We found that antibodies to ...Epstein-Barr virus (EBV) causes infectious mononucleosis and is associated with epithelial-cell cancers and B cell lymphomas. An effective EBV vaccine is not available. We found that antibodies to the EBV glycoprotein gH/gL complex were the principal components in human plasma that neutralized infection of epithelial cells and that antibodies to gH/gL and gp42 contributed to B cell neutralization. Immunization of mice and nonhuman primates with nanoparticle vaccines that displayed components of the viral-fusion machinery EBV gH/gL or gH/gL/gp42 elicited antibodies that potently neutralized both epithelial-cell and B cell infection. Immune serum from nonhuman primates inhibited EBV-glycoprotein-mediated fusion of epithelial cells and B cells and targeted an epitope critical for virus-cell fusion. Therefore, unlike the leading EBV gp350 vaccine candidate, which only protects B cells from infection, these EBV nanoparticle vaccines elicit antibodies that inhibit the virus-fusion apparatus and provide cell-type-independent protection from virus infection. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_7798.map.gz | 3.6 MB | EMDB map data format | |
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Header (meta data) | emd-7798-v30.xml emd-7798.xml | 9.6 KB 9.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_7798_fsc.xml | 3.6 KB | Display | FSC data file |
Images | emd_7798.png | 77.8 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-7798 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-7798 | HTTPS FTP |
-Validation report
Summary document | emd_7798_validation.pdf.gz | 78.2 KB | Display | EMDB validaton report |
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Full document | emd_7798_full_validation.pdf.gz | 77.3 KB | Display | |
Data in XML | emd_7798_validation.xml.gz | 494 B | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-7798 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-7798 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_7798.map.gz / Format: CCP4 / Size: 3.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | primary map | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 5.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : gH/gL/gp42-ferritin
Entire | Name: gH/gL/gp42-ferritin |
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Components |
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-Supramolecule #1: gH/gL/gp42-ferritin
Supramolecule | Name: gH/gL/gp42-ferritin / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5 |
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Source (natural) | Organism: Human herpesvirus 4 (Epstein-Barr virus) |
Recombinant expression | Organism: Homo sapiens (human) / Recombinant cell: HeLa / Recombinant plasmid: CMV/R 8kb VRC 8405 |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 1 mg/mL |
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Buffer | pH: 7.4 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 36.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |