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- EMDB-7559: Bacteriophage A511 baseplate in post-host attachment state (urea-... -
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Open data
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Basic information
Entry | Database: EMDB / ID: 7559 |
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Title | Bacteriophage A511 baseplate in post-host attachment state (urea-induced) |
![]() | Bacteriophage A511 in post-host attachment contracted state (urea-induced) |
![]() | Listeria virus A511: virus ![]() |
Source | ![]() ![]() |
Method | ![]() ![]() |
![]() | Guerrero-Ferreira R |
![]() | Journal: EMBO J. / Year: 2019 Title: Structure and transformation of bacteriophage A511 baseplate and tail upon infection of cells. ![]() Abstract: Contractile injection systems (bacteriophage tails, type VI secretions system, R-type pyocins, etc.) utilize a rigid tube/contractile sheath assembly for breaching the envelope of bacterial and ...Contractile injection systems (bacteriophage tails, type VI secretions system, R-type pyocins, etc.) utilize a rigid tube/contractile sheath assembly for breaching the envelope of bacterial and eukaryotic cells. Among contractile injection systems, bacteriophages that infect Gram-positive bacteria represent the least understood members. Here, we describe the structure of bacteriophage A511 tail in its pre- and post-host attachment states (extended and contracted, respectively) using cryo-electron microscopy, cryo-electron tomography, and X-ray crystallography. We show that the structure of the tube-baseplate complex of A511 is similar to that of phage T4, but the A511 baseplate is decorated with different receptor-binding proteins, which undergo a large structural transformation upon host attachment and switch the symmetry of the baseplate-tail fiber assembly from threefold to sixfold. For the first time under native conditions, we show that contraction of the phage tail sheath assembly starts at the baseplate and propagates through the sheath in a domino-like motion. |
Date | Deposition: Mar 16, 2018 / Header (metadata) release: Mar 28, 2018 / Map release: Jan 23, 2019 / Last update: Feb 13, 2019 |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
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Header (meta data in XML format) | ![]() ![]() ![]() |
FSC (resolution estimation) | ![]() |
Images | ![]() |
FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-7559 |
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Links
-Related structure data
Related structure data | ![]() C7560 ![]() C7561 ![]() C6hhk C: citing same article |
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Similar-shape strucutres |
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Map
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.27 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire Listeria virus A511
Entire | Name: Listeria virus A511 Details: Propagated in Listeria ivanovii strain WSLC 3009 (SV 5) Number of components: 1 |
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-Component #1: virus, Listeria virus A511
Virus | Name: Listeria virus A511 / Class: VIRION Details: Propagated in Listeria ivanovii strain WSLC 3009 (SV 5) Empty: No / Enveloped: No / Isolate: SPECIES |
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Species | Species: ![]() ![]() |
Source (natural) | Host Species: ![]() |
-Experimental details
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Sample preparation
Specimen | Specimen state: particle / Method: ![]() |
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Sample solution | Buffer solution: SM buffer: 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 8 mM MgSO4 pH: 7.5 |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 298 K / Humidity: 100 % |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Tecnai F20 / Image courtesy: FEI Company |
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![]() | Microscope: FEI TECNAI F20 |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: GATAN LIQUID NITROGEN |
Camera | Detector: FEI EAGLE (4k x 4k) |