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- EMDB-75376: Unknown entry -

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Basic information

Entry
Database: EMDB / ID: EMD-75376
TitleAsymmetric architecture and adaptation of Treponema flagella
Map data
Sample
  • Cell: Flagellar filament of Treponema denticola
    • Protein or peptide: Flagellin
KeywordsTreponema / Flagellin / Motility / Supercoil / Motor Protein
Biological speciesTreponema denticola ATCC 35405 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.72 Å
AuthorsWang J / Kurniyati K / Guo W / Botting JM / Sindelar CV / Li C / Liu J
Funding support United States, 5 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI087946 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI132818 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI078958 United States
National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR)R01DE023080 United States
National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR)R01DE034063 United States
CitationJournal: Nat Commun / Year: 2026
Title: Asymmetric architecture and adaptation of Treponema flagella.
Authors: Wang J / Kurniyati K / Guo W / Botting JM / Wu H / Sindelar CV / Li C / Liu J
History
DepositionJan 30, 2026-
Header (metadata) releaseJul 1, 2026-
Map releaseJul 1, 2026-
UpdateJul 1, 2026-
Current statusJul 1, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75376.png

Downloads & links

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Map

FileReleased
Voxel sizeX=Y=Z: 1.068 Å
Density
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.11659412 - 0.35485545
Average (Standard dev.)0.001458179 (±0.027742278)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 384.47998 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Flagellar filament of Treponema denticola

EntireName: Flagellar filament of Treponema denticola
Components
  • Cell: Flagellar filament of Treponema denticola
    • Protein or peptide: Flagellin

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Supramolecule #1: Flagellar filament of Treponema denticola

SupramoleculeName: Flagellar filament of Treponema denticola / type: cell / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)

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Macromolecule #1: Flagellin

MacromoleculeName: Flagellin / type: protein_or_peptide / ID: 1 / Number of copies: 11 / Enantiomer: LEVO
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)
Molecular weightTheoretical: 30.845812 KDa
SequenceString: MIINHNMSAM FAQRQGGVNE LHLAKNIEKL SSAQRINRAG DDASGLAVSE KMRSQIRGLN QAGQNIQNGV SFIQATEGYL GETTDIIQR LRELAIQASN GIYSAEDRMQ IQVEVSQLVD EVDRIASHAQ FNGMNILTGR FAQDSVTGPM QLHVGANMDQ R EKIYIGTM ...String:
MIINHNMSAM FAQRQGGVNE LHLAKNIEKL SSAQRINRAG DDASGLAVSE KMRSQIRGLN QAGQNIQNGV SFIQATEGYL GETTDIIQR LRELAIQASN GIYSAEDRMQ IQVEVSQLVD EVDRIASHAQ FNGMNILTGR FAQDSVTGPM QLHVGANMDQ R EKIYIGTM TATALGIIGA QQGGEDKMIS MSSVDGANMA LGALDNALKQ INKQRADLGA YQNRFEMAYD GIAIAAENMQ AA ESRIRDA DMAKEIVDYT KNQILIQSGT AMLAQANAQP QAVVRLL

UniProtKB: Flagellin

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statecell

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE-PROPANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 70.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.6 µm
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.72 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 112507
Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER

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