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- EMDB-75374: Asymmetric architecture and adaptation of Treponema flagella -

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Basic information

Entry
Database: EMDB / ID: EMD-75374
TitleAsymmetric architecture and adaptation of Treponema flagella
Map data
Sample
  • Complex: Flagellar filament of Treponema denticola
    • Protein or peptide: Flagellin
    • Protein or peptide: Flagellin
    • Protein or peptide: HEAT repeat domain-containing protein
    • Protein or peptide: Flagellar filament outer layer protein FlaA, putative
    • Protein or peptide: Flagellar filament outer layer protein FlaA, putative
    • Protein or peptide: Flagellar filament outer layer protein FlaA, putative
    • Protein or peptide: Flagellar filament outer layer protein FlaA, putative
    • Protein or peptide: Flagellar filament outer layer protein
KeywordsTreponema / Flagellin / Motility / Supercoil / Motor protein
Biological speciesTreponema denticola ATCC 35405 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.44 Å
AuthorsWang J / Kurniyati K / Guo W / Botting JM / Sindelar CV / Li C / Liu J
Funding support United States, 5 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI087946 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI132818 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI078958 United States
National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR)R01DE023080 United States
National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR)R01DE034063 United States
CitationJournal: Nat Commun / Year: 2026
Title: Asymmetric architecture and adaptation of Treponema flagella.
Authors: Jiaqi Wang / Kurni Kurniyati / Wangbiao Guo / Jack M Botting / Hui Wu / Charles V Sindelar / Chunhao Li / Jun Liu /
Abstract: Spirochetes exhibit a distinctive corkscrew-like motility driven by periplasmic flagella that wrap around the cell body in a supercoiled configuration, yet the structural basis of this propulsion ...Spirochetes exhibit a distinctive corkscrew-like motility driven by periplasmic flagella that wrap around the cell body in a supercoiled configuration, yet the structural basis of this propulsion remains poorly understood. Here we combine cryo-electron microscopy, cryo-electron tomography, and genetic and biochemical analyses to determine the assembly and adaptation principles of the supercoiled flagellar filament in Treponema denticola, a major periodontal pathogen. Near-atomic structures reveal a glycosylated FlaB flagellin core encased by an asymmetric sheath. The major sheath protein FlaA forms the bulk of the sheath and mechanically couples to the core through defined interfaces required for efficient motility, whereas four minor sheath proteins (FlaA1, FlaA2, FlaAP1, and FlaAP2) assemble along the concave side of the filament to accommodate intrinsic curvature. Disruption of this asymmetric core-sheath organization compromises force transmission and impairs motility, establishing coordinated asymmetric assembly as a fundamental mechanism underlying spirochetal motility.
History
DepositionJan 30, 2026-
Header (metadata) releaseJul 1, 2026-
Map releaseJul 1, 2026-
UpdateJul 1, 2026-
Current statusJul 1, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75374.png

Downloads & links

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Map

FileDownload / File: emd_75374.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 360 pix.
= 384.48 Å		1.07 Å/pix.
x 360 pix.
= 384.48 Å		1.07 Å/pix.
x 360 pix.
= 384.48 Å

Surface

Projections

Slices (1/3)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.068 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.2661489 - 0.4218419
Average (Standard dev.)0.004656098 (±0.031851843)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 384.47998 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_75374_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_75374_half_map_2.map
Projections & Slices
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Histogram (log scale)

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Sample components

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Entire : Flagellar filament of Treponema denticola

EntireName: Flagellar filament of Treponema denticola
Components
  • Complex: Flagellar filament of Treponema denticola
    • Protein or peptide: Flagellin
    • Protein or peptide: Flagellin
    • Protein or peptide: HEAT repeat domain-containing protein
    • Protein or peptide: Flagellar filament outer layer protein FlaA, putative
    • Protein or peptide: Flagellar filament outer layer protein FlaA, putative
    • Protein or peptide: Flagellar filament outer layer protein FlaA, putative
    • Protein or peptide: Flagellar filament outer layer protein FlaA, putative
    • Protein or peptide: Flagellar filament outer layer protein

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Supramolecule #1: Flagellar filament of Treponema denticola

SupramoleculeName: Flagellar filament of Treponema denticola / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)

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Macromolecule #1: Flagellin

MacromoleculeName: Flagellin / type: protein_or_peptide / ID: 1 / Number of copies: 11 / Enantiomer: LEVO
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)
Molecular weightTheoretical: 31.342555 KDa
SequenceString: MIINHNMSAM FAQRTQGVTN VRIGKDIEKL SSGLRINRAG DDASGLAVSE KMRSQIRGLN QASANASNGI NFIQVAEAFL QETTDIMQR IRELAVQASN GIYSAEDRMQ IQVEVSQLVA EVDRIASSAQ FNGMNMLTGR FARETGENVV TGSMWFHIGA N MDQRMRVY ...String:
MIINHNMSAM FAQRTQGVTN VRIGKDIEKL SSGLRINRAG DDASGLAVSE KMRSQIRGLN QASANASNGI NFIQVAEAFL QETTDIMQR IRELAVQASN GIYSAEDRMQ IQVEVSQLVA EVDRIASSAQ FNGMNMLTGR FARETGENVV TGSMWFHIGA N MDQRMRVY IGTMSAAALG IRDIGDEKIM TIETADAANR SIGTIDEGLK KINKQRADLG GYQNRMELTV VGIDIAAENL QA AESRIRD ADMAKQMVEY TKNQILSNTG IAMLAQANNN SQLVMSLLR

UniProtKB: Flagellin

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Macromolecule #2: Flagellin

MacromoleculeName: Flagellin / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)
Molecular weightTheoretical: 12.48743 KDa
SequenceString:
VYYVNVQILK IFTHSDGYYI IYRRAGLKHA EVFIPKSWLD PKDGRARLEL VNTRVNPYLS FYLKDGKFDR IKIAAPRDLG SPIWGVLKD PNKYNGKFDG VESLELKF

UniProtKB: TP-0437 domain-containing protein

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Macromolecule #3: HEAT repeat domain-containing protein

MacromoleculeName: HEAT repeat domain-containing protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)
Molecular weightTheoretical: 21.413689 KDa
SequenceString: NSMEGIILRE MVLSEGRDAK FVALQVIEEA IEGGRVTPEL QEALDSLATV GLTSVVRENG RLANNYPDVR REACRLLGKV KNEQAKKSL MTVMYTDNEP VVIMEAVKSL GELGFNNNDE VVDMINFINR KFDIINPTSS LALEVLNAYE KLAPTVKNKR G MTESIMRI ...String:
NSMEGIILRE MVLSEGRDAK FVALQVIEEA IEGGRVTPEL QEALDSLATV GLTSVVRENG RLANNYPDVR REACRLLGKV KNEQAKKSL MTVMYTDNEP VVIMEAVKSL GELGFNNNDE VVDMINFINR KFDIINPTSS LALEVLNAYE KLAPTVKNKR G MTESIMRI ANNFNYITPV RNRALEVLKS IMAG

UniProtKB: HEAT repeat domain-containing protein

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Macromolecule #4: Flagellar filament outer layer protein FlaA, putative

MacromoleculeName: Flagellar filament outer layer protein FlaA, putative / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)
Molecular weightTheoretical: 23.017166 KDa
SequenceString: RIKEVSIEKF ETEGTWYAMM SADEGVVRAR LFNGNPLNKK PIPAEEGLEL ADEKVLGVKV SFYRRGYNSF EVHATKALPV EGIAKTASV WVVGRSYPHT MKLIVEDFWG KRFELYVGKL NHSGWKLMTV AIPPQNAAGK TGIIQKDYHY GTSMGLKIVG F RVECDPEE ...String:
RIKEVSIEKF ETEGTWYAMM SADEGVVRAR LFNGNPLNKK PIPAEEGLEL ADEKVLGVKV SFYRRGYNSF EVHATKALPV EGIAKTASV WVVGRSYPHT MKLIVEDFWG KRFELYVGKL NHSGWKLMTV AIPPQNAAGK TGIIQKDYHY GTSMGLKIVG F RVECDPEE AYGHYYIYFD DLRVVSDLYE VDMRDQDDMS DNW

UniProtKB: Flagellar filament outer layer protein FlaA, putative

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Macromolecule #5: Flagellar filament outer layer protein FlaA, putative

MacromoleculeName: Flagellar filament outer layer protein FlaA, putative / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)
Molecular weightTheoretical: 23.751732 KDa
SequenceString: SINYQTYMVD TFDNPDGQDW AYQAVGSKFI TEGYPKVQYL NGMPHAVKVM HDDAENAKFL GLEFKFNRKG DNWVDVVPTK DGKPYEIPF KGNIHRLDMW VWGAGYYYNI EILVRDCEGR THALPLGYVN FKGWKNMHVT VPTNIPQASR YIGNKNKMTF V AFRIRTAP ...String:
SINYQTYMVD TFDNPDGQDW AYQAVGSKFI TEGYPKVQYL NGMPHAVKVM HDDAENAKFL GLEFKFNRKG DNWVDVVPTK DGKPYEIPF KGNIHRLDMW VWGAGYYYNI EILVRDCEGR THALPLGYVN FKGWKNMHVT VPTNIPQASR YIGNKNKMTF V AFRIRTAP FERVDSFKIF FDEFKALTDV YVDSYDGYEL ADQSFD

UniProtKB: Flagellar filament outer layer protein FlaA, putative

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Macromolecule #6: Flagellar filament outer layer protein FlaA, putative

MacromoleculeName: Flagellar filament outer layer protein FlaA, putative / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)
Molecular weightTheoretical: 23.693697 KDa
SequenceString: GSINYQTYMV DTFDNPDGQD WAYQAVGSKF ITEGYPKVQY LNGMPHAVKV MHDDAENAKF LGLEFKFNRK GDNWVDVVPT KDGKPYEIP FKGNIHRLDM WVWGAGYYYN IEILVRDCEG RTHALPLGYV NFKGWKNMHV TVPTNIPQAS RYIGNKNKMT F VAFRIRTA ...String:
GSINYQTYMV DTFDNPDGQD WAYQAVGSKF ITEGYPKVQY LNGMPHAVKV MHDDAENAKF LGLEFKFNRK GDNWVDVVPT KDGKPYEIP FKGNIHRLDM WVWGAGYYYN IEILVRDCEG RTHALPLGYV NFKGWKNMHV TVPTNIPQAS RYIGNKNKMT F VAFRIRTA PFERVDSFKI FFDEFKALTD VYVDSYDGYE LADQSF

UniProtKB: Flagellar filament outer layer protein FlaA, putative

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Macromolecule #7: Flagellar filament outer layer protein FlaA, putative

MacromoleculeName: Flagellar filament outer layer protein FlaA, putative / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)
Molecular weightTheoretical: 25.093322 KDa
SequenceString: DFSTIDAADP NKVGVDTAEQ RIKEVSIEKF ETEGTWYAMM SADEGVVRAR LFNGNPLNKK PIPAEEGLEL ADEKVLGVKV SFYRRGYNS FEVHATKALP VEGIAKTASV WVVGRSYPHT MKLIVEDFWG KRFELYVGKL NHSGWKLMTV AIPPQNAAGK T GIIQKDYH ...String:
DFSTIDAADP NKVGVDTAEQ RIKEVSIEKF ETEGTWYAMM SADEGVVRAR LFNGNPLNKK PIPAEEGLEL ADEKVLGVKV SFYRRGYNS FEVHATKALP VEGIAKTASV WVVGRSYPHT MKLIVEDFWG KRFELYVGKL NHSGWKLMTV AIPPQNAAGK T GIIQKDYH YGTSMGLKIV GFRVECDPEE AYGHYYIYFD DLRVVSDLYE VDMRDQDDMS DNW

UniProtKB: Flagellar filament outer layer protein FlaA, putative

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Macromolecule #8: Flagellar filament outer layer protein

MacromoleculeName: Flagellar filament outer layer protein / type: protein_or_peptide / ID: 8 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)Organism: Treponema denticola ATCC 35405 (bacteria)
Molecular weightTheoretical: 36.868539 KDa
SequenceString: EEAIIIDFAL LNADIIADPN GKMTQNRRTV MDYGKVAGAS YTDEQKALMR TSLALEQWDV ELNSSAQNPL SVATSTIKEA EVRAEGEKF AGQKLMGVRI LFPEWTNNAN AKIKPGFLIP AYEKMAQVDD QGNLQEPTAE DKASGKSRFE DGYGVVRNTG V IKSIAVNT ...String:
EEAIIIDFAL LNADIIADPN GKMTQNRRTV MDYGKVAGAS YTDEQKALMR TSLALEQWDV ELNSSAQNPL SVATSTIKEA EVRAEGEKF AGQKLMGVRI LFPEWTNNAN AKIKPGFLIP AYEKMAQVDD QGNLQEPTAE DKASGKSRFE DGYGVVRNTG V IKSIAVNT YGMNFPHGLY VLLRDQNNVV KRYFMGYLLF DGWRELVWNN PSYIANVKSR ELRLYPVYPT ALPHVAFEGF LI TRDAAHD GGDAIAYFKD VKIIYDKAVL TTVRDFADED LWGIQEERET KRKKIEVEKF GHTQVLRFLE QEKMATEEGF TPS EGSEKN

UniProtKB: Flagellar filament outer layer protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE-PROPANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 70.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.6 µm
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.44 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 159002
Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER

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