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- EMDB-75264: HSV-2 gHecto/gL -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-75264
TitleHSV-2 gHecto/gL
Map dataHSV-2 gHecto/gL half map 1
Sample
  • Complex: HSV-2 gHecto/gL heterodimer
    • Protein or peptide: Envelope glycoprotein L
    • Protein or peptide: Envelope glycoprotein H
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
KeywordsHerpes Simplex Virus 2 glycoprotein / fusion regulator / VIRAL PROTEIN
Function / homology
Function and homology information


host cell endosome membrane / host cell Golgi apparatus / fusion of virus membrane with host plasma membrane / viral envelope / symbiont entry into host cell / host cell plasma membrane / virion membrane
Similarity search - Function
Herpesvirus glycoprotein L, C-terminal / dsDNA virus glycoprotein L C terminal / Herpesvirus glycoprotein L, N-terminal / Herpesvirus glycoprotein L, N-terminal domain superfamily / Herpesvirus glycoprotein L / Alphaherpesvirus glycoprotein L (gL) domain profile. / Envelope glycoprotein L / Herpesvirus glycoprotein H main domain / Herpesvirus glycoprotein H / Herpesvirus glycoprotein H, C-terminal ...Herpesvirus glycoprotein L, C-terminal / dsDNA virus glycoprotein L C terminal / Herpesvirus glycoprotein L, N-terminal / Herpesvirus glycoprotein L, N-terminal domain superfamily / Herpesvirus glycoprotein L / Alphaherpesvirus glycoprotein L (gL) domain profile. / Envelope glycoprotein L / Herpesvirus glycoprotein H main domain / Herpesvirus glycoprotein H / Herpesvirus glycoprotein H, C-terminal / Herpesvirus glycoprotein H, C-terminal domain superfamily / Herpesvirus glycoprotein H C-terminal domain
Similarity search - Domain/homology
Envelope glycoprotein L / Envelope glycoprotein H
Similarity search - Component
Biological speciesHuman alphaherpesvirus 2
Methodsingle particle reconstruction / cryo EM / Resolution: 3.71 Å
AuthorsHeldwein EE / Gonzalez-Del Pino GL / Walsh RM
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI164698 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)K12 GM133314 United States
Howard Hughes Medical Institute (HHMI)55108533 United States
CitationJournal: To Be Published
Title: HSV-2 gHecto/gL
Authors: Heldwein EE / Gonzalez-Del Pino GL / Walsh RM
History
DepositionJan 26, 2026-
Header (metadata) releaseSep 30, 2026-
Map releaseSep 30, 2026-
UpdateSep 30, 2026-
Current statusSep 30, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75264.png

Downloads & links

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Map

FileDownload / File: emd_75264.map.gz / Format: CCP4 / Size: 371.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationHSV-2 gHecto/gL half map 1
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.74 Å/pix.
x 460 pix.
= 339.02 Å		0.74 Å/pix.
x 460 pix.
= 339.02 Å		0.74 Å/pix.
x 460 pix.
= 339.02 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.737 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.014
Minimum - Maximum-0.043726064 - 0.0888678
Average (Standard dev.)0.000026171383 (±0.0013298292)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions460460460
Spacing460460460
CellA=B=C: 339.02 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: HSV-2 gHecto/gL half map 1

Fileemd_75264_half_map_1.map
AnnotationHSV-2 gHecto/gL half map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: HSV-2 gHecto/gL half map 1

Fileemd_75264_half_map_2.map
AnnotationHSV-2 gHecto/gL half map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : HSV-2 gHecto/gL heterodimer

EntireName: HSV-2 gHecto/gL heterodimer
Components
  • Complex: HSV-2 gHecto/gL heterodimer
    • Protein or peptide: Envelope glycoprotein L
    • Protein or peptide: Envelope glycoprotein H
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

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Supramolecule #1: HSV-2 gHecto/gL heterodimer

SupramoleculeName: HSV-2 gHecto/gL heterodimer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Human alphaherpesvirus 2
Molecular weightTheoretical: 125 KDa

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Macromolecule #1: Envelope glycoprotein L

MacromoleculeName: Envelope glycoprotein L / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human alphaherpesvirus 2
Molecular weightTheoretical: 25.229676 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MGFVCLFGLV VMGAWGAWGG SQATEYVLRS VIAKEVGDIL RVPCMRTPAD DVSWRYEAPS VIDYARIDGI FLRYHCPGLD TFLWDRHAQ RAYLVNPFLF AAGFLEDLSH SVFPADTQET TTRRALYKEI RDALGSRKQA VSHAPVRAGC VNFDYSRTRR C VGRRDLRP ...String:
MGFVCLFGLV VMGAWGAWGG SQATEYVLRS VIAKEVGDIL RVPCMRTPAD DVSWRYEAPS VIDYARIDGI FLRYHCPGLD TFLWDRHAQ RAYLVNPFLF AAGFLEDLSH SVFPADTQET TTRRALYKEI RDALGSRKQA VSHAPVRAGC VNFDYSRTRR C VGRRDLRP ANTTSTWEPP VSSDDEASSQ SKPLATQPPV LALSNAPPRR VSPTRGRRRH TRLRRN

UniProtKB: Envelope glycoprotein L

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Macromolecule #2: Envelope glycoprotein H

MacromoleculeName: Envelope glycoprotein H / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human alphaherpesvirus 2
Molecular weightTheoretical: 86.887961 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MGPGLWVVMG VLVGVAGGHD TYWTEQIDPW FLHGLGLART YWRDTNTGRL WLPNTPDASD PQRGRLAPPG ELNLTTASVP MLRWYAERF CFVLVTTAEF PRDPGQLLYI PKTYLLGRPR NASLPELPEA GPTSRPPAEV TQLKGLSHNP GASALLRSRA W VTFAAAPD ...String:
MGPGLWVVMG VLVGVAGGHD TYWTEQIDPW FLHGLGLART YWRDTNTGRL WLPNTPDASD PQRGRLAPPG ELNLTTASVP MLRWYAERF CFVLVTTAEF PRDPGQLLYI PKTYLLGRPR NASLPELPEA GPTSRPPAEV TQLKGLSHNP GASALLRSRA W VTFAAAPD REGLTFPRGD DGATERHPDG RRNAPPPGPP AGTPRHPTTN LSIAHLHNAS VTWLAARGLL RTPGRYVYLS PS ASTWPVG VWTTGGLAFG CDAALVRARY GKGFMGLVIS MRDSPPAEII VVPADKTLAR VGNPTDENAP AVLPGPPAGP RYR VFVLGA PTPADNGSAL DALRRVAGYP EESTNYAQYM SRAYAEFLGE DPGSGTDARP SLFWRLAGLL ASSGFAFVNA AHAH DAIRL SDLLGFLAHS RVLAGLAARG AAGCAADSVF LNVSVLDPAA RLRLEARLGH LVAAILEREQ SLVAHALGYQ LAFVL DSPA AYGAVAPSAA RLIDALYAEF LGGRALTAPM VRRALFYATA VLRAPFLAGA PSAEQRERAR RGLLITTALC TSDVAA ATH ADLRAALART DHQKNLFWLP DHFSPCAASL RFDLAEGGFI LDALAMATRS DIPADVMAQQ TRGVASVLTR WAHYNAL IR AFVPEATHQC SGPSHNAEPR ILVPITHNAS YVVTHTPLPR GIGYKLTGVD VRRPLFITYL TATCEGHARE IEPKRLVR T ENRRDLGLVG AVFLRYTPAG EVMSVLLVDT DATQQQLAQG PVAGTPNVFS SDVPSVALLL FPNGTVIHLL AFDTLPIAT IAPHHHHHH

UniProtKB: Envelope glycoprotein H

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Macromolecule #4: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 4 / Number of copies: 5 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1 mg/mL
BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 52.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3m1c

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.71 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 128490
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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