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- EMDB-75188: HSV-2 gHecto/gL/CHL2-Fab complex -

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Basic information

Entry
Database: EMDB / ID: EMD-75188
TitleHSV-2 gHecto/gL/CHL2-Fab complex
Map datagH2gL2-CHL2 complex
Sample
  • Complex: HSV-2 gHecto/gL/CHL2-Fab complex
    • Protein or peptide: Envelope glycoprotein H
    • Protein or peptide: Envelope glycoprotein L
    • Protein or peptide: CHL2-heavy chain
    • Protein or peptide: CHL2-light chain
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
  • Ligand: water
KeywordsHerpes Simplex Virus 2 glycoprotein / fusion regulator / antibody-bound / VIRAL PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


host cell endosome membrane / host cell Golgi apparatus / fusion of virus membrane with host plasma membrane / viral envelope / symbiont entry into host cell / host cell plasma membrane / virion membrane
Similarity search - Function
Herpesvirus glycoprotein L, C-terminal / dsDNA virus glycoprotein L C terminal / Herpesvirus glycoprotein L, N-terminal / Herpesvirus glycoprotein L, N-terminal domain superfamily / Herpesvirus glycoprotein L / Alphaherpesvirus glycoprotein L (gL) domain profile. / Envelope glycoprotein L / Herpesvirus glycoprotein H main domain / Herpesvirus glycoprotein H / Herpesvirus glycoprotein H, C-terminal ...Herpesvirus glycoprotein L, C-terminal / dsDNA virus glycoprotein L C terminal / Herpesvirus glycoprotein L, N-terminal / Herpesvirus glycoprotein L, N-terminal domain superfamily / Herpesvirus glycoprotein L / Alphaherpesvirus glycoprotein L (gL) domain profile. / Envelope glycoprotein L / Herpesvirus glycoprotein H main domain / Herpesvirus glycoprotein H / Herpesvirus glycoprotein H, C-terminal / Herpesvirus glycoprotein H, C-terminal domain superfamily / Herpesvirus glycoprotein H C-terminal domain
Similarity search - Domain/homology
Envelope glycoprotein L / Envelope glycoprotein H
Similarity search - Component
Biological speciesHuman alphaherpesvirus 2 / Mus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.92 Å
AuthorsHeldwein EE / Gonzalez-Del Pino GL / Walsh RM
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI164698 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)K12 GM133314 United States
Howard Hughes Medical Institute (HHMI)55108533 United States
CitationJournal: To Be Published
Title: HSV-2 gHecto/gL/CHL2-Fab complex
Authors: Heldwein EE / Gonzalez-Del Pino GL / Walsh RM
History
DepositionJan 21, 2026-
Header (metadata) releaseSep 30, 2026-
Map releaseSep 30, 2026-
UpdateSep 30, 2026-
Current statusSep 30, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75188.png

Downloads & links

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Map

FileDownload / File: emd_75188.map.gz / Format: CCP4 / Size: 371.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationgH2gL2-CHL2 complex
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.94 Å/pix.
x 460 pix.
= 432.4 Å		0.94 Å/pix.
x 460 pix.
= 432.4 Å		0.94 Å/pix.
x 460 pix.
= 432.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.94 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.026
Minimum - Maximum-0.10857669 - 0.23657823
Average (Standard dev.)-0.0000068832414 (±0.0027102001)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions460460460
Spacing460460460
CellA=B=C: 432.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: gH2gL2-CHL2 complex half 2

Fileemd_75188_half_map_1.map
AnnotationgH2gL2-CHL2 complex half 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: gH2gL2-CHL2 complex half 1

Fileemd_75188_half_map_2.map
AnnotationgH2gL2-CHL2 complex half 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : HSV-2 gHecto/gL/CHL2-Fab complex

EntireName: HSV-2 gHecto/gL/CHL2-Fab complex
Components
  • Complex: HSV-2 gHecto/gL/CHL2-Fab complex
    • Protein or peptide: Envelope glycoprotein H
    • Protein or peptide: Envelope glycoprotein L
    • Protein or peptide: CHL2-heavy chain
    • Protein or peptide: CHL2-light chain
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
  • Ligand: water

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Supramolecule #1: HSV-2 gHecto/gL/CHL2-Fab complex

SupramoleculeName: HSV-2 gHecto/gL/CHL2-Fab complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4
Source (natural)Organism: Human alphaherpesvirus 2
Molecular weightTheoretical: 185 KDa

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Macromolecule #1: Envelope glycoprotein H

MacromoleculeName: Envelope glycoprotein H / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human alphaherpesvirus 2
Molecular weightTheoretical: 86.887961 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MGPGLWVVMG VLVGVAGGHD TYWTEQIDPW FLHGLGLART YWRDTNTGRL WLPNTPDASD PQRGRLAPPG ELNLTTASVP MLRWYAERF CFVLVTTAEF PRDPGQLLYI PKTYLLGRPR NASLPELPEA GPTSRPPAEV TQLKGLSHNP GASALLRSRA W VTFAAAPD ...String:
MGPGLWVVMG VLVGVAGGHD TYWTEQIDPW FLHGLGLART YWRDTNTGRL WLPNTPDASD PQRGRLAPPG ELNLTTASVP MLRWYAERF CFVLVTTAEF PRDPGQLLYI PKTYLLGRPR NASLPELPEA GPTSRPPAEV TQLKGLSHNP GASALLRSRA W VTFAAAPD REGLTFPRGD DGATERHPDG RRNAPPPGPP AGTPRHPTTN LSIAHLHNAS VTWLAARGLL RTPGRYVYLS PS ASTWPVG VWTTGGLAFG CDAALVRARY GKGFMGLVIS MRDSPPAEII VVPADKTLAR VGNPTDENAP AVLPGPPAGP RYR VFVLGA PTPADNGSAL DALRRVAGYP EESTNYAQYM SRAYAEFLGE DPGSGTDARP SLFWRLAGLL ASSGFAFVNA AHAH DAIRL SDLLGFLAHS RVLAGLAARG AAGCAADSVF LNVSVLDPAA RLRLEARLGH LVAAILEREQ SLVAHALGYQ LAFVL DSPA AYGAVAPSAA RLIDALYAEF LGGRALTAPM VRRALFYATA VLRAPFLAGA PSAEQRERAR RGLLITTALC TSDVAA ATH ADLRAALART DHQKNLFWLP DHFSPCAASL RFDLAEGGFI LDALAMATRS DIPADVMAQQ TRGVASVLTR WAHYNAL IR AFVPEATHQC SGPSHNAEPR ILVPITHNAS YVVTHTPLPR GIGYKLTGVD VRRPLFITYL TATCEGHARE IEPKRLVR T ENRRDLGLVG AVFLRYTPAG EVMSVLLVDT DATQQQLAQG PVAGTPNVFS SDVPSVALLL FPNGTVIHLL AFDTLPIAT IAPHHHHHH

UniProtKB: Envelope glycoprotein H

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Macromolecule #2: Envelope glycoprotein L

MacromoleculeName: Envelope glycoprotein L / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human alphaherpesvirus 2
Molecular weightTheoretical: 25.229676 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MGFVCLFGLV VMGAWGAWGG SQATEYVLRS VIAKEVGDIL RVPCMRTPAD DVSWRYEAPS VIDYARIDGI FLRYHCPGLD TFLWDRHAQ RAYLVNPFLF AAGFLEDLSH SVFPADTQET TTRRALYKEI RDALGSRKQA VSHAPVRAGC VNFDYSRTRR C VGRRDLRP ...String:
MGFVCLFGLV VMGAWGAWGG SQATEYVLRS VIAKEVGDIL RVPCMRTPAD DVSWRYEAPS VIDYARIDGI FLRYHCPGLD TFLWDRHAQ RAYLVNPFLF AAGFLEDLSH SVFPADTQET TTRRALYKEI RDALGSRKQA VSHAPVRAGC VNFDYSRTRR C VGRRDLRP ANTTSTWEPP VSSDDEASSQ SKPLATQPPV LALSNAPPRR VSPTRGRRRH TRLRRN

UniProtKB: Envelope glycoprotein L

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Macromolecule #3: CHL2-heavy chain

MacromoleculeName: CHL2-heavy chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 51.117473 KDa
Recombinant expressionOrganism: Mus musculus (house mouse)
SequenceString: MRVLILLWLF TAFPGILSDV QLQESGPGLV KPSQSLSLTC TVTGYSITSD YAWNWIRQFP GNKLEWMGYI SYSGSTGYNP SFKSRISIT RDTSKNQFFL QLKSVTTEDT ATYYCSRSDY YGYAMDYWGQ GTSVTVSSAK TTPPSVYPLA PGSAQTNSAV T LGCLVKGY ...String:
MRVLILLWLF TAFPGILSDV QLQESGPGLV KPSQSLSLTC TVTGYSITSD YAWNWIRQFP GNKLEWMGYI SYSGSTGYNP SFKSRISIT RDTSKNQFFL QLKSVTTEDT ATYYCSRSDY YGYAMDYWGQ GTSVTVSSAK TTPPSVYPLA PGSAQTNSAV T LGCLVKGY FPEPVTVTWN SGSLSSGVHT FPAVLQSDLY TLSSSVTVPS STWPSETVTC NVAHPASSTK VDKKIVPRDC GC KPCICTV PEVSSVFIFP PKPKDVLTIT LTPKVTCVVV DISKDDPEVQ FSWFVDDVEV HTAQTQPREE QFNSTFRSVS ELP IMHQDW LNGKEFKCRV NSAAFPAPIE KTISKTKGRP KAPQVYTIPP PKEQMAKDKV SLTCMITDFF PEDITVEWQW NGQP AENYK NTQPIMDTDG SYFVYSKLNV QKSNWEAGNT FTCSVLHEGL HNHHTEKSLS HSPGK

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Macromolecule #4: CHL2-light chain

MacromoleculeName: CHL2-light chain / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 26.49367 KDa
Recombinant expressionOrganism: Mus musculus (house mouse)
SequenceString: MDMRTPAQFL GILLLWFPGI KCDIKMTQSP SSMYASLGER VTITCKASQD INSYLSWFQQ KPGKSPKTLI YRTDRLIDGV PSRFSGSGS GQDYSLTISS LEYEDMGIYY CQQYDEFPLT FGAGTKLELK RADAAPTVSI FPPSSEQLTS GGASVVCFLN N FYPKDINV ...String:
MDMRTPAQFL GILLLWFPGI KCDIKMTQSP SSMYASLGER VTITCKASQD INSYLSWFQQ KPGKSPKTLI YRTDRLIDGV PSRFSGSGS GQDYSLTISS LEYEDMGIYY CQQYDEFPLT FGAGTKLELK RADAAPTVSI FPPSSEQLTS GGASVVCFLN N FYPKDINV KWKIDGSERQ NGVLNSWTDQ DSKDSTYSMS STLTLTKDEY ERHNSYTCEA THKTSTSPIV KSFNRNEC

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Macromolecule #6: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 6 / Number of copies: 5 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Macromolecule #7: water

MacromoleculeName: water / type: ligand / ID: 7 / Number of copies: 6 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1 mg/mL
BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 52.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3m1c

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.92 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 363706
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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