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- EMDB-75095: Cbf1-CCAN-CEN complex -

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Basic information

Entry
Database: EMDB / ID: EMD-75095
TitleCbf1-CCAN-CEN complex
Map data
Sample
  • Complex: Cbf1-CCAN-CEN complex
    • Complex: Cbf1 dimer
      • Protein or peptide: x 1 types
    • Complex: CCAN
      • Protein or peptide: x 13 types
    • Complex: CEN3 DNA
      • DNA: x 2 types
Keywordskinetochore / centromere / DNA binding / CELL CYCLE
Function / homology
Function and homology information


Cbf1-Met4-Met28 complex / positive regulation of sulfate assimilation / regulation of sulfur metabolic process / negative regulation of kinetochore assembly / positive regulation of inositol biosynthetic process / negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination / COMA complex / maintenance of meiotic sister chromatid cohesion / negative regulation of ceramide biosynthetic process / meiotic sister chromatid segregation ...Cbf1-Met4-Met28 complex / positive regulation of sulfate assimilation / regulation of sulfur metabolic process / negative regulation of kinetochore assembly / positive regulation of inositol biosynthetic process / negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination / COMA complex / maintenance of meiotic sister chromatid cohesion / negative regulation of ceramide biosynthetic process / meiotic sister chromatid segregation / Mis6-Sim4 complex / establishment of meiotic sister chromatid cohesion / ascospore formation / attachment of spindle microtubules to kinetochore / centromeric DNA binding / CENP-A containing chromatin assembly / protein localization to chromosome, centromeric region / kinetochore assembly / outer kinetochore / establishment of mitotic sister chromatid cohesion / cellular response to methionine / mitotic spindle assembly checkpoint signaling / chromosome, centromeric region / DNA replication initiation / meiotic cell cycle / chromosome segregation / kinetochore / DNA-binding transcription repressor activity, RNA polymerase II-specific / DNA-binding transcription activator activity, RNA polymerase II-specific / RNA polymerase II-specific DNA-binding transcription factor binding / protein dimerization activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin remodeling / DNA-binding transcription factor activity / cell division / regulation of transcription by RNA polymerase II / protein-containing complex binding / structural molecule activity / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / mitochondrion / nucleus
Similarity search - Function
: / Kinetochore subunit Nkp2/Cnl2 / Cnl2/NKP2 family protein / : / CENPH protein / Centromere protein O / Cenp-O kinetochore centromere component / Centromere protein Chl4/mis15/CENP-N / Centromere protein Chl4/mis15/CENP-N / Helix-loop-helix DNA-binding domain ...: / Kinetochore subunit Nkp2/Cnl2 / Cnl2/NKP2 family protein / : / CENPH protein / Centromere protein O / Cenp-O kinetochore centromere component / Centromere protein Chl4/mis15/CENP-N / Centromere protein Chl4/mis15/CENP-N / Helix-loop-helix DNA-binding domain / helix loop helix domain / Myc-type, basic helix-loop-helix (bHLH) domain / Myc-type, basic helix-loop-helix (bHLH) domain profile. / Helix-loop-helix DNA-binding domain superfamily
Similarity search - Domain/homology
: / Centromere-binding protein 1 / Inner kinetochore subunit IML3 / Inner kinetochore subunit CHL4 / Inner kinetochore subunit CNN1 / Inner kinetochore subunit MCM22 / Inner kinetochore subunit OKP1 / Inner kinetochore subunit CTF19 / Inner kinetochore subunit NKP2 / Inner kinetochore subunit MCM21 ...: / Centromere-binding protein 1 / Inner kinetochore subunit IML3 / Inner kinetochore subunit CHL4 / Inner kinetochore subunit CNN1 / Inner kinetochore subunit MCM22 / Inner kinetochore subunit OKP1 / Inner kinetochore subunit CTF19 / Inner kinetochore subunit NKP2 / Inner kinetochore subunit MCM21 / Inner kinetochore subunit MCM16 / Inner kinetochore subunit NKP1 / Inner kinetochore subunit CTF3 / Inner kinetochore subunit WIP1
Similarity search - Component
Biological speciesEscherichia phage 933W (virus) / Saccharomyces cerevisiae (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsMengqiu J / Sue B
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: To Be Published
Title: Native yeast kinetochore structures identify an essential inner kinetochore interaction
Authors: Mengqiu J / Sue B
History
DepositionJan 14, 2026-
Header (metadata) releaseJul 8, 2026-
Map releaseJul 8, 2026-
UpdateJul 8, 2026-
Current statusJul 8, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75095.png

Downloads & links

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Map

FileReleased
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 400 pix.
= 428. Å		1.07 Å/pix.
x 400 pix.
= 428. Å		1.07 Å/pix.
x 400 pix.
= 428. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.14
Minimum - Maximum-0.29770142 - 0.73911536
Average (Standard dev.)0.0011005654 (±0.02131676)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 428.00003 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_75095_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_75095_half_map_2.map
Projections & Slices
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Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Cbf1-CCAN-CEN complex

EntireName: Cbf1-CCAN-CEN complex
Components
  • Complex: Cbf1-CCAN-CEN complex
    • Complex: Cbf1 dimer
      • Protein or peptide: Centromere-binding protein 1
    • Complex: CCAN
      • Protein or peptide: Inner kinetochore subunit MCM16
      • Protein or peptide: Inner kinetochore subunit CTF3
      • Protein or peptide: Inner kinetochore subunit MCM22
      • Protein or peptide: Inner kinetochore subunit IML3
      • Protein or peptide: Inner kinetochore subunit CHL4
      • Protein or peptide: Inner kinetochore subunit MCM21
      • Protein or peptide: Inner kinetochore subunit CTF19
      • Protein or peptide: Inner kinetochore subunit OKP1
      • Protein or peptide: Inner kinetochore subunit CNN1
      • Protein or peptide: K7_Ame1p
      • Protein or peptide: Inner kinetochore subunit WIP1
      • Protein or peptide: Inner kinetochore subunit NKP1
      • Protein or peptide: Inner kinetochore subunit NKP2
    • Complex: CEN3 DNA
      • DNA: DNA (508-MER)
      • DNA: DNA (508-MER)

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Supramolecule #1: Cbf1-CCAN-CEN complex

SupramoleculeName: Cbf1-CCAN-CEN complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#16
Source (natural)Organism: Escherichia phage 933W (virus)

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Supramolecule #2: Cbf1 dimer

SupramoleculeName: Cbf1 dimer / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #3: CCAN

SupramoleculeName: CCAN / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #3-#15
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #4: CEN3 DNA

SupramoleculeName: CEN3 DNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #2, #16
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast) / Synthetically produced: Yes

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Macromolecule #1: Centromere-binding protein 1

MacromoleculeName: Centromere-binding protein 1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 39.444715 KDa
SequenceString: MNSLANNNKL STEDEEIHSA RKRGYNEEQN YSEARKKQRD QGLLSQESND GNIDSALLSE GATLKGTQSQ YESGLTSNKD EKGSDDEDA SVAEAAVAAT VNYTDLIQGQ EDSSDAHTSN QTNANGEHKD SLNGERAITP SNEGVKPNTS LEGMTSSPME S TQQSKNDM ...String:
MNSLANNNKL STEDEEIHSA RKRGYNEEQN YSEARKKQRD QGLLSQESND GNIDSALLSE GATLKGTQSQ YESGLTSNKD EKGSDDEDA SVAEAAVAAT VNYTDLIQGQ EDSSDAHTSN QTNANGEHKD SLNGERAITP SNEGVKPNTS LEGMTSSPME S TQQSKNDM LIPLAEHDRG PEHQQDDEDN DDADIDLKKD ISMQPGRRGR KPTTLATTDE WKKQRKDSHK EVERRRRENI NT AINVLSD LLPVRESSKA AILACAAEYI QKLKETDEAN IEKWTLQKLL SEQNASQLAS ANEKLQEELG NAYKEIEYMK RVL RKEGIE YEDMHTHKKQ ENERKSTRSD NPHEA

UniProtKB: Centromere-binding protein 1

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Macromolecule #3: Inner kinetochore subunit MCM16

MacromoleculeName: Inner kinetochore subunit MCM16 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 21.1661 KDa
SequenceString:
MTNSSEKQWE RIQQLEKEHV EVYRELLITL DRLYLIRKHN HAVILSHTQQ RLLEIRHQLQ INLEKTALLI RLLEKPDNTN VLFTKLQNL LEESNSLDYE LLQSLGAQSS LHKQLIESRA ERDELMSKLI ELSSKFPKPT IPPDDSDTAG KQVEVEKENE T IQELMIAL QIHSGYTNIS YTI

UniProtKB: Inner kinetochore subunit MCM16

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Macromolecule #4: Inner kinetochore subunit CTF3

MacromoleculeName: Inner kinetochore subunit CTF3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 84.345633 KDa
SequenceString: MSLILDDIIL SLTNANERTP PQALKTTLSL LYEKSKQYGL SSPQLQALVR LLCETSIIDT VTKVYIVENC FLPDGYLTKE LLLEIINHL GTPTVFSRYR IQTPPVLQSA LCKWLVHVYF LFPVHSEREH NISSSIWLHL WQFSFLQKWI TPLVIWQATT P VDVKPWKL ...String:
MSLILDDIIL SLTNANERTP PQALKTTLSL LYEKSKQYGL SSPQLQALVR LLCETSIIDT VTKVYIVENC FLPDGYLTKE LLLEIINHL GTPTVFSRYR IQTPPVLQSA LCKWLVHVYF LFPVHSEREH NISSSIWLHL WQFSFLQKWI TPLVIWQATT P VDVKPWKL SIIKRCAMHP GYRDAPGSAT LILQRFQCLV GASSQITESI ITINCNRKTL KSHRNLKLDA HFLSILKRIL SR AHPANFP ADTVQNTIDM YLSEIHQLGA DSIYPLRLQS LPEYVPSDST VSLWDVTSLE QLAQNWPQLH IPNDVDYMMK PSL NSNVLL PRKVMSRDSL KHLYSSIILI KNSRDESSSP YEWCIWQLKR CFAHQIETPQ EVIPIIISVS SMDNKLSSRI IQTF CNLKY LKLDELTLKK VCGGILPLWK PELISGTREF FVKFMASIFM WSTRDGHDNN CTFSETCFYV LQMITNWVLD DKLIA LGLT LLHDMQSLLT LDKIFNNATS NRFSTMAFIS SLDILTQLSK QTKSDYAIQY LIVGPDIMNK VFSSDDPLLL SAACRY LVA TKNKLMQYPS TNKFVRMQNQ YIMDLTNYLY RNKVLSSKSL FGVSPDFFKQ ILENLYIPTA DFKNAKFFTI TGIPALS YI CIIILRRLET AENTKIKFTS GIINEETFNN FFRVHHDEIG QHGWIKGVNN IHDLRVKILM HLSNTANPYR DIAAFLFT Y LKSLSKYSVQ NS

UniProtKB: Inner kinetochore subunit CTF3

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Macromolecule #5: Inner kinetochore subunit MCM22

MacromoleculeName: Inner kinetochore subunit MCM22 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 27.602541 KDa
SequenceString: MDVEKDVLDV YIKNLENQIG NKRYFLKQAQ GAIDEITKRS LDTEGKPVNS EVFTELLRKP MFFSERADPI GFSLTSNFLS LRAQSSSEW LSLMNDQSVD QKAMLLLQNN INSDLKELLR KLQHQMTIMD SKKQDHAHIR TRKARNKELW DSLADFLKGY L VPNLDDND ...String:
MDVEKDVLDV YIKNLENQIG NKRYFLKQAQ GAIDEITKRS LDTEGKPVNS EVFTELLRKP MFFSERADPI GFSLTSNFLS LRAQSSSEW LSLMNDQSVD QKAMLLLQNN INSDLKELLR KLQHQMTIMD SKKQDHAHIR TRKARNKELW DSLADFLKGY L VPNLDDND ESIDSLTNEV MLLMKRLIEH DLNLTLNDFS SKTIPIYRLL LRANIITVIE GSTNPGTKYI KLIDFNETSL T

UniProtKB: Inner kinetochore subunit MCM22

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Macromolecule #6: Inner kinetochore subunit IML3

MacromoleculeName: Inner kinetochore subunit IML3 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 28.093223 KDa
SequenceString: MPYTWKFLGI SKQLSLENGI AKLNQLLNLE VDLDIQTIRV PSDPDGGTAA DEYIRYEMRL DISNLDEGTY SKFIFLGNSK MEVPMFLCY CGTDNRNEVV LQWLKAEYGV IMWPIKFEQK TMIKLADASI VHVTKENIEQ ITWFSSKLYF EPETQDKNLR Q FSIEIPRE ...String:
MPYTWKFLGI SKQLSLENGI AKLNQLLNLE VDLDIQTIRV PSDPDGGTAA DEYIRYEMRL DISNLDEGTY SKFIFLGNSK MEVPMFLCY CGTDNRNEVV LQWLKAEYGV IMWPIKFEQK TMIKLADASI VHVTKENIEQ ITWFSSKLYF EPETQDKNLR Q FSIEIPRE SCEGLALGYG NTMHPYNDAI VPYIYNETGM AVERLPLTSV ILAGHTKIMR ESIVTSTRSL RNRVLAVVLQ SI QFTSE

UniProtKB: Inner kinetochore subunit IML3

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Macromolecule #7: Inner kinetochore subunit CHL4

MacromoleculeName: Inner kinetochore subunit CHL4 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 52.743723 KDa
SequenceString: MSNELRLEDN YVPTSDTLVV FKQLMKLPVT VLYDLTLSWF AKFGGSFDGD IYLLTETLDL LIEKGVRRNV IVNRILYVYW PDGLNVFQL AEIDCHLMIS KPEKFKWLPS KALRGDGKPY VVKLQPAKFI ENLQTDLAKI YHCHVYMFKH PSLPVLITRI Q LFDSNNLF ...String:
MSNELRLEDN YVPTSDTLVV FKQLMKLPVT VLYDLTLSWF AKFGGSFDGD IYLLTETLDL LIEKGVRRNV IVNRILYVYW PDGLNVFQL AEIDCHLMIS KPEKFKWLPS KALRGDGKPY VVKLQPAKFI ENLQTDLAKI YHCHVYMFKH PSLPVLITRI Q LFDSNNLF LSTPNIGSIN KESLYNKLDK FQGKPLISRR PYYVAFPLNS PIIFHSVDKD IYARLVLQSI SRTISERETI IF KPVQKIP VKSIHNIMTL LGPSRFAESM GPWECYASAN FERSPLHDYK KHQGLTGKKV MVREFDDSFL NDDENFYGKE EPE IRRLRL EKNMIKFKGS ANGVMDQKYN DLKEFNEHVH NIRNGKKNED SGEPVYISRY SSLVPIEKVG FTLKNEINSR IITI KLKFN GNDIFGGLHE LCDKNLINID KVPGWLAGEN GSFSGTIMNG DFQREQVAKG GLL

UniProtKB: Inner kinetochore subunit CHL4

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Macromolecule #8: Inner kinetochore subunit MCM21

MacromoleculeName: Inner kinetochore subunit MCM21 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 43.028879 KDa
SequenceString: MSRIDDLQQD IESLLSEINS LEESREKLKA KIKDKRKNEE SANPIVQEFE DLFDQFPQLN NFLFNEHPEL EETDDKDISR AQADIPATP IPYEPKKRAK LENEEILPEQ EWVLKTQPMV QHQMFDPGVA DLLDTDILTS PSKRKRKLKI DDISTSDRSE L EDYIVLEN ...String:
MSRIDDLQQD IESLLSEINS LEESREKLKA KIKDKRKNEE SANPIVQEFE DLFDQFPQLN NFLFNEHPEL EETDDKDISR AQADIPATP IPYEPKKRAK LENEEILPEQ EWVLKTQPMV QHQMFDPGVA DLLDTDILTS PSKRKRKLKI DDISTSDRSE L EDYIVLEN VYRMFGITFF PLVDPIDLKI KDASGEIFVD REMLGIRLEV FSERTSQFEK PHYVLLKKRI KSNSWFLFKH TI PSFIDVQ GIFDDTNGGL VISHDDAYLF AKRVFLQLVE VQKRRQIFKD LEAKKIIHDL DLDLESSMVS FFVKDIKVEL FVK QNEIVS CSILDDIHDF SQNNKSKWEI ALLGSLDDLE LKLNHSFATI FK

UniProtKB: Inner kinetochore subunit MCM21

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Macromolecule #9: Inner kinetochore subunit CTF19

MacromoleculeName: Inner kinetochore subunit CTF19 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 42.841113 KDa
SequenceString: MDFTSDTTNS HDTSNSHLSL EDAVGTHHAG EADVNIDGDE KQQLSLLDDD QVRALKLQEE KDALLTRRNT LLQEIQTYQN ILMKENNSK TKNGDILQND ITQDFLNLIS ISSSNPNSAI SDRKRVERIN GLTNLQKELV TKYDTLPLLN MNLRLSYLRD H TYPHLQVS ...String:
MDFTSDTTNS HDTSNSHLSL EDAVGTHHAG EADVNIDGDE KQQLSLLDDD QVRALKLQEE KDALLTRRNT LLQEIQTYQN ILMKENNSK TKNGDILQND ITQDFLNLIS ISSSNPNSAI SDRKRVERIN GLTNLQKELV TKYDTLPLLN MNLRLSYLRD H TYPHLQVS VQSRDRVHND GIEVLVVNYK FCRNTMNPFE IQFKMFYKFE DSTLLKWEIL RISTNVRLKA KQLLATRNFQ KC LLSLYEF DKIKSKKTGI FQNLINLLKR KTRCYLMNNS DSLIVERVIR EGRLTTIKLQ INFIITMPGE RGKPRNCFLP MSK ISIALW KGGERFNQID LDEICYGLIK EYGVKTGLKE ICNVCLFPDM YAR

UniProtKB: Inner kinetochore subunit CTF19

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Macromolecule #10: Inner kinetochore subunit OKP1

MacromoleculeName: Inner kinetochore subunit OKP1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 47.427246 KDa
SequenceString: MAADRDNFLQ NIENDSINNG QAMDLSPNRS SSESDSSILM NVNDIKTLRL DVAPEAKSTQ SKKSLFYENS DDAEEGEIEE RTNKEEGQY HHKGSKQLRF EVGKESTGKL QSHLSDGSAT SGEGNVRPWE FRKVIQAEYR ERLPRNYELK HWKKPSKIMI G SILRLLET ...String:
MAADRDNFLQ NIENDSINNG QAMDLSPNRS SSESDSSILM NVNDIKTLRL DVAPEAKSTQ SKKSLFYENS DDAEEGEIEE RTNKEEGQY HHKGSKQLRF EVGKESTGKL QSHLSDGSAT SGEGNVRPWE FRKVIQAEYR ERLPRNYELK HWKKPSKIMI G SILRLLET NTVSALDSVF EKYEKEMNQM THGDNNEVKR IYSKKERLLE IILTKIKKKL RQAKFPSRIS ERDLDIEYIY SK RQFIQNR YSQELQNNER LEAILSREQN LLEETRKLCM NLKTNNKKRL TEKLIQKDLH PVLNKAMEYT YGLESTNGFM HPD GPVTFR NDSHELNLML NDPIKSTADV RLDKEEVLSL LPSLKEYTKK SKELKETMGQ MISDSHEEEI KEVFVPHHES HQDK TEEDI H

UniProtKB: Inner kinetochore subunit OKP1

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Macromolecule #11: Inner kinetochore subunit CNN1

MacromoleculeName: Inner kinetochore subunit CNN1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 41.359785 KDa
SequenceString: MSTPRKAAGN NENTEVSEIR TPFRERALEE QRLKDEVLIR NTPGYRKLLS ASTKSHDILN KDPNEVRSFL QDLSQVLARK SQGNDTTTN KTQARNLIDE LAYEESQPEE NELLRSRSEK LTDNNIGNET QPDYTSLSQT VFAKLQERDK GLKSRKIDPI I IQDVPTTG ...String:
MSTPRKAAGN NENTEVSEIR TPFRERALEE QRLKDEVLIR NTPGYRKLLS ASTKSHDILN KDPNEVRSFL QDLSQVLARK SQGNDTTTN KTQARNLIDE LAYEESQPEE NELLRSRSEK LTDNNIGNET QPDYTSLSQT VFAKLQERDK GLKSRKIDPI I IQDVPTTG HEDELTVHSP DKANSISMEV LRTSPSIGMD QVDEPPVRDP VPISITQQEE PLSEDLPSDD KEETEEAENE DY SFENTSD ENLDDIGNDP IRLNVPAVRR SSIKPLQIMD LKHLTRQFLN ENRIILPKQT WSTIQEESLN IMDFLKQKIG TLQ KQELVD SFIDMGIINN VDDMFELAHE LLPLELQSRI ESYLF

UniProtKB: Inner kinetochore subunit CNN1

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Macromolecule #12: K7_Ame1p

MacromoleculeName: K7_Ame1p / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 37.562742 KDa
SequenceString: MDRDTKLAFR LRGSHSRRTD DIDDDVIVFK TPNAVYREEN SPIQSPVQPI LSSPKLANSF EFPITTNNVN AQDRHEHGYQ PLDAEDYPM IDSENKSPIS ESPQNVRNDE DLTTRYNFDD IPIRQLSSSI TSVTTIDVLS SLFINLFEND LIPQALKDFN K SDDDQFRK ...String:
MDRDTKLAFR LRGSHSRRTD DIDDDVIVFK TPNAVYREEN SPIQSPVQPI LSSPKLANSF EFPITTNNVN AQDRHEHGYQ PLDAEDYPM IDSENKSPIS ESPQNVRNDE DLTTRYNFDD IPIRQLSSSI TSVTTIDVLS SLFINLFEND LIPQALKDFN K SDDDQFRK LLYKLDLRLF QTISDQMTRD LKDILDINVS NNELCYQLKQ VLARKEDLNQ QIISVRNEIQ ELKAGKDWHD LQ NEQAKLN DKVKLNKRLN DLTSTLLGKY EEDRKIMSQD SEDDSIRDDS NILDIAHFVD LMDPYNGLLK KINKINENLS NEL QPSL

UniProtKB: UNIPROTKB: G2W9L4

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Macromolecule #13: Inner kinetochore subunit WIP1

MacromoleculeName: Inner kinetochore subunit WIP1 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 10.255458 KDa
SequenceString:
MDTEALANYL LRQLSLDAEE NKLEDLLQRQ NEDQESSQEY NKKLLLACGF QAILRKILLD ARTRATAEGL REVYPYHIEA ATQAFLDSQ

UniProtKB: Inner kinetochore subunit WIP1

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Macromolecule #14: Inner kinetochore subunit NKP1

MacromoleculeName: Inner kinetochore subunit NKP1 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 27.006451 KDa
SequenceString: MTDTYNSISN FIENELTALL SSDDYLMDDL AGELPNEVCR LLKAQVIEKR KDAMSRGKQD LLSKEIYDNE SELRASQSQQ IMELVGDIP KYSLGSELRN RVEGEPQSTS IERLIEDVLK LPQMEVADEE EVEVENDLKV LSEYSNLRKD LILKCQALQI G ESKLSDIL ...String:
MTDTYNSISN FIENELTALL SSDDYLMDDL AGELPNEVCR LLKAQVIEKR KDAMSRGKQD LLSKEIYDNE SELRASQSQQ IMELVGDIP KYSLGSELRN RVEGEPQSTS IERLIEDVLK LPQMEVADEE EVEVENDLKV LSEYSNLRKD LILKCQALQI G ESKLSDIL SQTNSINSLT TSIKEASEDD DISEYFATYN GKLVVALEEM KLLLEEAVKT FGNSPEKREK IKKILSELKK

UniProtKB: Inner kinetochore subunit NKP1

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Macromolecule #15: Inner kinetochore subunit NKP2

MacromoleculeName: Inner kinetochore subunit NKP2 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 17.877033 KDa
SequenceString:
MNSEQLLHNY VSDSLLTTLI SFQEFKQQLQ SYTSDEQQLQ HWYELLQARD ARVTSELEAR IKQFFITLRS RLLRFLESEQ LSHSLSLET LIDALYKIND LLQQRLQILD DAIQEKTSEL AEFENMVRSP SAGDNAIPGL LQIIQSYINL LEEN

UniProtKB: Inner kinetochore subunit NKP2

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Macromolecule #2: DNA (508-MER)

MacromoleculeName: DNA (508-MER) / type: dna / ID: 2 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 156.413875 KDa
SequenceString: (DA)(DT)(DT)(DG)(DT)(DT)(DT)(DG)(DT)(DG) (DC)(DA)(DC)(DT)(DT)(DG)(DC)(DC)(DT)(DG) (DC)(DA)(DG)(DG)(DC)(DC)(DT)(DT)(DT) (DT)(DG)(DA)(DA)(DA)(DA)(DG)(DC)(DA)(DA) (DG) (DC)(DA)(DT)(DA)(DA)(DA) ...String:
(DA)(DT)(DT)(DG)(DT)(DT)(DT)(DG)(DT)(DG) (DC)(DA)(DC)(DT)(DT)(DG)(DC)(DC)(DT)(DG) (DC)(DA)(DG)(DG)(DC)(DC)(DT)(DT)(DT) (DT)(DG)(DA)(DA)(DA)(DA)(DG)(DC)(DA)(DA) (DG) (DC)(DA)(DT)(DA)(DA)(DA)(DA)(DG) (DA)(DT)(DC)(DT)(DA)(DA)(DA)(DC)(DA)(DT) (DA)(DA) (DA)(DA)(DT)(DC)(DT)(DG)(DT) (DA)(DA)(DA)(DA)(DT)(DA)(DA)(DC)(DA)(DA) (DG)(DA)(DT) (DG)(DT)(DA)(DA)(DA)(DG) (DA)(DT)(DA)(DA)(DT)(DG)(DC)(DT)(DA)(DA) (DA)(DT)(DC)(DA) (DT)(DT)(DT)(DG)(DG) (DC)(DT)(DT)(DT)(DT)(DT)(DG)(DA)(DT)(DT) (DG)(DA)(DT)(DT)(DG) (DT)(DA)(DC)(DA) (DG)(DG)(DA)(DA)(DA)(DA)(DT)(DA)(DT)(DA) (DC)(DA)(DT)(DC)(DG)(DC) (DA)(DG)(DG) (DG)(DG)(DG)(DT)(DT)(DG)(DA)(DC)(DT)(DT) (DT)(DT)(DA)(DC)(DC)(DA)(DT) (DT)(DT) (DC)(DA)(DC)(DC)(DG)(DC)(DA)(DA)(DT)(DG) (DG)(DA)(DA)(DT)(DC)(DA)(DA)(DA) (DC) (DT)(DT)(DG)(DT)(DT)(DG)(DA)(DA)(DG)(DA) (DG)(DA)(DA)(DT)(DG)(DT)(DT)(DC)(DA) (DC)(DA)(DG)(DG)(DC)(DG)(DC)(DA)(DT)(DA) (DC)(DG)(DC)(DT)(DA)(DC)(DA)(DA)(DT)(DG) (DA)(DC)(DC)(DC)(DG)(DA)(DT)(DT)(DC) (DT)(DT)(DG)(DC)(DT)(DA)(DG)(DC)(DC)(DT) (DT) (DT)(DT)(DC)(DT)(DC)(DG)(DG)(DT) (DC)(DT)(DT)(DG)(DC)(DA)(DA)(DA)(DC)(DA) (DA)(DC) (DC)(DG)(DC)(DC)(DG)(DG)(DC) (DC)(DT)(DT)(DC)(DC)(DA)(DC)(DC)(DA)(DG) (DT)(DA)(DA) (DA)(DC)(DG)(DT)(DT)(DT) (DC)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DC)(DA) (DT)(DT)(DC)(DA) (DA)(DT)(DG)(DA)(DA) (DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DT)(DT) (DC)(DT)(DT)(DA)(DC) (DT)(DA)(DT)(DT) (DT)(DC)(DT)(DT)(DT)(DT)(DT)(DT)(DA)(DA) (DC)(DT)(DT)(DT)(DC)(DG) (DG)(DA)(DA) (DA)(DT)(DC)(DA)(DA)(DA)(DT)(DA)(DC)(DA) (DC)(DT)(DA)(DA)(DT)(DA)(DT) (DT)(DT) (DT)(DA)(DA)(DA)(DT)(DT)(DT)(DT)(DA)(DT) (DT)(DT)(DT)(DT)(DT)(DA)(DA)(DA) (DA) (DA)(DT)(DA)(DA)(DA)(DC)(DT)(DA)(DC)(DT) (DT)(DT)(DT)(DT)(DA)(DT)(DT)(DT)(DT) (DT)(DT)(DA)(DC)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DA)(DA)(DA)(DA)(DA)(DT)(DA)(DT)(DA) (DA)(DT)(DA)(DA)(DA)(DA)(DT)(DC)(DA) (DA)(DA)(DT)(DA)(DT)(DC)(DA)(DT)(DC)(DA) (DT) (DG)(DT)(DG)(DA)(DC)(DT)(DT)(DA) (DT)(DT)(DT)(DG)(DT)(DA)(DC)(DT)(DA)(DT) (DT)(DT) (DT)(DT)(DT)(DC)(DA)(DA)(DT) (DG)(DA)(DA)(DT)(DA)(DG)(DC)(DT)(DT)(DT) (DC)(DT)(DG) (DT)(DG)(DG)(DA)(DT)(DT) (DT)(DT)(DC)(DC)(DA)(DT)(DA)(DT)(DT)(DG) (DT)(DT)(DT)(DG) (DG)(DC)(DG)(DC)(DT) (DG)(DA)(DT)

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Macromolecule #16: DNA (508-MER)

MacromoleculeName: DNA (508-MER) / type: dna / ID: 16 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 157.288578 KDa
SequenceString: (DA)(DT)(DC)(DA)(DG)(DC)(DG)(DC)(DC)(DA) (DA)(DA)(DC)(DA)(DA)(DT)(DA)(DT)(DG)(DG) (DA)(DA)(DA)(DA)(DT)(DC)(DC)(DA)(DC) (DA)(DG)(DA)(DA)(DA)(DG)(DC)(DT)(DA)(DT) (DT) (DC)(DA)(DT)(DT)(DG)(DA) ...String:
(DA)(DT)(DC)(DA)(DG)(DC)(DG)(DC)(DC)(DA) (DA)(DA)(DC)(DA)(DA)(DT)(DA)(DT)(DG)(DG) (DA)(DA)(DA)(DA)(DT)(DC)(DC)(DA)(DC) (DA)(DG)(DA)(DA)(DA)(DG)(DC)(DT)(DA)(DT) (DT) (DC)(DA)(DT)(DT)(DG)(DA)(DA)(DA) (DA)(DA)(DA)(DT)(DA)(DG)(DT)(DA)(DC)(DA) (DA)(DA) (DT)(DA)(DA)(DG)(DT)(DC)(DA) (DC)(DA)(DT)(DG)(DA)(DT)(DG)(DA)(DT)(DA) (DT)(DT)(DT) (DG)(DA)(DT)(DT)(DT)(DT) (DA)(DT)(DT)(DA)(DT)(DA)(DT)(DT)(DT)(DT) (DT)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DG) (DT)(DA)(DA)(DA)(DA)(DA)(DA)(DT)(DA)(DA) (DA)(DA)(DA)(DG)(DT) (DA)(DG)(DT)(DT) (DT)(DA)(DT)(DT)(DT)(DT)(DT)(DA)(DA)(DA) (DA)(DA)(DA)(DT)(DA)(DA) (DA)(DA)(DT) (DT)(DT)(DA)(DA)(DA)(DA)(DT)(DA)(DT)(DT) (DA)(DG)(DT)(DG)(DT)(DA)(DT) (DT)(DT) (DG)(DA)(DT)(DT)(DT)(DC)(DC)(DG)(DA)(DA) (DA)(DG)(DT)(DT)(DA)(DA)(DA)(DA) (DA) (DA)(DG)(DA)(DA)(DA)(DT)(DA)(DG)(DT)(DA) (DA)(DG)(DA)(DA)(DA)(DT)(DA)(DT)(DA) (DT)(DA)(DT)(DT)(DT)(DC)(DA)(DT)(DT)(DG) (DA)(DA)(DT)(DG)(DG)(DA)(DT)(DA)(DT)(DA) (DT)(DG)(DA)(DA)(DA)(DC)(DG)(DT)(DT) (DT)(DA)(DC)(DT)(DG)(DG)(DT)(DG)(DG)(DA) (DA) (DG)(DG)(DC)(DC)(DG)(DG)(DC)(DG) (DG)(DT)(DT)(DG)(DT)(DT)(DT)(DG)(DC)(DA) (DA)(DG) (DA)(DC)(DC)(DG)(DA)(DG)(DA) (DA)(DA)(DA)(DG)(DG)(DC)(DT)(DA)(DG)(DC) (DA)(DA)(DG) (DA)(DA)(DT)(DC)(DG)(DG) (DG)(DT)(DC)(DA)(DT)(DT)(DG)(DT)(DA)(DG) (DC)(DG)(DT)(DA) (DT)(DG)(DC)(DG)(DC) (DC)(DT)(DG)(DT)(DG)(DA)(DA)(DC)(DA)(DT) (DT)(DC)(DT)(DC)(DT) (DT)(DC)(DA)(DA) (DC)(DA)(DA)(DG)(DT)(DT)(DT)(DG)(DA)(DT) (DT)(DC)(DC)(DA)(DT)(DT) (DG)(DC)(DG) (DG)(DT)(DG)(DA)(DA)(DA)(DT)(DG)(DG)(DT) (DA)(DA)(DA)(DA)(DG)(DT)(DC) (DA)(DA) (DC)(DC)(DC)(DC)(DC)(DT)(DG)(DC)(DG)(DA) (DT)(DG)(DT)(DA)(DT)(DA)(DT)(DT) (DT) (DT)(DC)(DC)(DT)(DG)(DT)(DA)(DC)(DA)(DA) (DT)(DC)(DA)(DA)(DT)(DC)(DA)(DA)(DA) (DA)(DA)(DG)(DC)(DC)(DA)(DA)(DA)(DT)(DG) (DA)(DT)(DT)(DT)(DA)(DG)(DC)(DA)(DT)(DT) (DA)(DT)(DC)(DT)(DT)(DT)(DA)(DC)(DA) (DT)(DC)(DT)(DT)(DG)(DT)(DT)(DA)(DT)(DT) (DT) (DT)(DA)(DC)(DA)(DG)(DA)(DT)(DT) (DT)(DT)(DA)(DT)(DG)(DT)(DT)(DT)(DA)(DG) (DA)(DT) (DC)(DT)(DT)(DT)(DT)(DA)(DT) (DG)(DC)(DT)(DT)(DG)(DC)(DT)(DT)(DT)(DT) (DC)(DA)(DA) (DA)(DA)(DG)(DG)(DC)(DC) (DT)(DG)(DC)(DA)(DG)(DG)(DC)(DA)(DA)(DG) (DT)(DG)(DC)(DA) (DC)(DA)(DA)(DA)(DC) (DA)(DA)(DT)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6qle

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 11639
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

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