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- EMDB-75107: De novo assembled CBF3-CEN complex -

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Basic information

Entry
Database: EMDB / ID: EMD-75107
TitleDe novo assembled CBF3-CEN complex
Map data
Sample
  • Complex: CBF3-CEN complex
    • Protein or peptide: Centromere DNA-binding protein complex CBF3 subunit A
    • Protein or peptide: Centromere DNA-binding protein complex CBF3 subunit B
    • Protein or peptide: Centromere DNA-binding protein complex CBF3 subunit C
    • Protein or peptide: Suppressor of kinetochore protein 1
    • DNA: DNA (44-MER)
    • DNA: DNA (44-MER)
  • Ligand: ZINC ION
Keywordschromosome segregation / kinetochore / centromere / CELL CYCLE / CELL CYCLE-DNA complex
Biological speciesSaccharomyces cerevisiae (brewer's yeast) / synthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsMengqiu J / Sue B
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: To Be Published
Title: Native yeast kinetochore structures identify an essential inner kinetochore interaction
Authors: Mengqiu J / Sue B
History
DepositionJan 15, 2026-
Header (metadata) releaseJul 1, 2026-
Map releaseJul 1, 2026-
UpdateJul 1, 2026-
Current statusJul 1, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75107.png

Downloads & links

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Map

FileDownload / File: emd_75107.map.gz / Format: CCP4 / Size: 290.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 424 pix.
= 453.68 Å		1.07 Å/pix.
x 424 pix.
= 453.68 Å		1.07 Å/pix.
x 424 pix.
= 453.68 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.14
Minimum - Maximum-0.28016305 - 0.6569178
Average (Standard dev.)0.0009913628 (±0.01733375)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions424424424
Spacing424424424
CellA=B=C: 453.68002 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_75107_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_75107_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_75107_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : CBF3-CEN complex

EntireName: CBF3-CEN complex
Components
  • Complex: CBF3-CEN complex
    • Protein or peptide: Centromere DNA-binding protein complex CBF3 subunit A
    • Protein or peptide: Centromere DNA-binding protein complex CBF3 subunit B
    • Protein or peptide: Centromere DNA-binding protein complex CBF3 subunit C
    • Protein or peptide: Suppressor of kinetochore protein 1
    • DNA: DNA (44-MER)
    • DNA: DNA (44-MER)
  • Ligand: ZINC ION

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Supramolecule #1: CBF3-CEN complex

SupramoleculeName: CBF3-CEN complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#6
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: Centromere DNA-binding protein complex CBF3 subunit A

MacromoleculeName: Centromere DNA-binding protein complex CBF3 subunit A / type: protein_or_peptide / ID: 1 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 112.066031 KDa
SequenceString: MRSSILFLLK LMKIMDVQQQ QEAMSSEDRF QELVDSLKPR TAHQYKTYYT KYIQWCQLNQ IIPTPEDNSV NSVPYKDLPI SAELIHWFL LDTLITDDKP GEKREETEDL DEEEENSFKI ATLKKIIGSL NFLSKLCKVH ENPNANIDTK YLESVTKLHT H WIDSQKAI ...String:
MRSSILFLLK LMKIMDVQQQ QEAMSSEDRF QELVDSLKPR TAHQYKTYYT KYIQWCQLNQ IIPTPEDNSV NSVPYKDLPI SAELIHWFL LDTLITDDKP GEKREETEDL DEEEENSFKI ATLKKIIGSL NFLSKLCKVH ENPNANIDTK YLESVTKLHT H WIDSQKAI TTNETNNTNT QVLCPPLLKV SLNLWNPETN HLSEKFFKTC SEKLRFLVDF QLRSYLNLSF EERSKIRFGS LK LGKRDRD AIIYHKVTHS AEKKDTPGHH QLLALLPQDC PFICPQTTLA AYLYLRFYGI PSVSKGDGFP NLNADENGSL LQD IPILRG KSLTTYPREE TFSNYYTTVF RYCHLPYKRR EYFNKCNLVY PTWDEDTFRT FFNEENHGNW LEQPEAFAFP DKIP FDFKK IMNFKSPYTS YSTNAKKDPF PPPKDLLVQI FPEIDEYKRH DYEGLSQNSR DFLDLMEVLR ERFLSNLPWI YKFFP NHDI FQDPIFGNSD FQSYFNDKTI HSKGSPILSF DILPGFNKIY KNKTNFYSLL IERPSQLTFA SSHNPDTHPT QKQESE GPL QMSQLDTTQL NELLKQQSFE YVQFQTLSNF QILLSVFNKI FEKLEMKKSS RGYILHQLNL FKITLDERIK KSKIDDA DK FIRDNQPIKK EENIVNEDGP NTSRRTKRPK QIRLLSIADS SDESSTEDSN VFKKDGESIE DGAYGENEDE NDSEMQEQ L KSMINELINS KISTFLRDQM DQFELKINAL LDKILEEKVT RIIEQKLGSH TGKFSTLKRP QLYMTEEHNV GFDMEVPKK LRTSGKYAET VKDNDDHQAM STTASPSPEQ DQEAKSYTDE QEFMLDKSID SIEGIILEWF TPNAKYANQC VHSMNKSGNK SWRANCEAL YKERKSIVEF YIYLVNHESL DRYKAVDICE KLRDQNEGSF SRLAKFLRKW RHDHQNSFDG LLVYLSN

UniProtKB: Centromere DNA-binding protein complex CBF3 subunit A

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Macromolecule #2: Centromere DNA-binding protein complex CBF3 subunit B

MacromoleculeName: Centromere DNA-binding protein complex CBF3 subunit B / type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 71.439891 KDa
SequenceString: MFNRTTQLKS KHPCSVCTRR KVKCDRMIPC GNCRKRGQDS ECMKSTKLIT ASSSKEYLPD LLLFWQNYEY WITNIGLYKT KQRDLTRTP ANLDTDTEEC MFWMNYLQKD QSFQLMNFAM ENLGALYFGS IGDISELYLR VEQYWDRRAD KNHSVDGKYW D ALIWSVFT ...String:
MFNRTTQLKS KHPCSVCTRR KVKCDRMIPC GNCRKRGQDS ECMKSTKLIT ASSSKEYLPD LLLFWQNYEY WITNIGLYKT KQRDLTRTP ANLDTDTEEC MFWMNYLQKD QSFQLMNFAM ENLGALYFGS IGDISELYLR VEQYWDRRAD KNHSVDGKYW D ALIWSVFT MCIYYMPVEK LAEIFSVYPL HEYLGSNKRL NWEDGMQLVM CQNFARCSLF QLKQCDFMAH PDIRLVQAYL IL ATTTFPY DEPLLANSLL TQCIHTFKNF HVDDFRPLLN DDPVESIAKV TLGRIFYRLC GCDYLQSGPR KPIALHTEVS SLL QHAAYL QDLPNVDVYR EENSTEVLYW KIISLDRDLD QYLNKSSKPP LKTLDAIRRE LDIFQYKVDS LEEDFRSNNS RFQK FIALF QISTVSWKLF KMYLIYYDTA DSLLKVIHYS KVIISLIVNN FHAKSEFFNR HPMVMQTITR VVSFISFYQI FVESA AVKQ LLVDLTELTA NLPTIFGSKL DKLVYLTERL SKLKLLWDKV QLLDSGDSFY HPVFKILQND IKIIELKNDE MFSLIK GLG SLVPLNKLRQ ESLLEEEDEN NTEPSDFRTI VEEFQSEYNI SDILS

UniProtKB: Centromere DNA-binding protein complex CBF3 subunit B

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Macromolecule #3: Centromere DNA-binding protein complex CBF3 subunit C

MacromoleculeName: Centromere DNA-binding protein complex CBF3 subunit C / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 56.416863 KDa
SequenceString: MPSFNPVRFL ELPIDIRKEV YFHLDGNFCG AHPYPIDILY KSNDVELPGK PSYKRSKRSK KLLRYMYPVF ATYLNIFEYS PQLIEKWLE YAFWLRYDCL VLDCFKVNHL YDGTLIDALE WTYLDNELRL AYFNKASMLE VWYTFKEYKK WVIDSVAFDE L DLLNVSNI ...String:
MPSFNPVRFL ELPIDIRKEV YFHLDGNFCG AHPYPIDILY KSNDVELPGK PSYKRSKRSK KLLRYMYPVF ATYLNIFEYS PQLIEKWLE YAFWLRYDCL VLDCFKVNHL YDGTLIDALE WTYLDNELRL AYFNKASMLE VWYTFKEYKK WVIDSVAFDE L DLLNVSNI QFNIDNLTPQ LVDKCLSILE QKDLFATIGE VQFGQDEEVG EEKDVDVSGA NSDENSSPSS TIKNKKRSAS KR SHSDNGN VGATHNQLTS ISVIRTIRSM ESMKSLRKIT VRGEKLYELL INFHGFRDNP GKTISYIVKR RINEIRLSRM NQI SRTGLA DFTRWDNLQK LVLSRVAYID LNSIVFPKNF KSLTMKRVSK IKWWNIEENI LKELKVDKRT FKSLYIKEDD SKFT KFFNL RHTRIKELDK SEINQITYLR CQAIVWLSFR TLNHIKLQNV SEVFNNIIVP RALFDSKRVE IYRCEKISQV LVI

UniProtKB: Centromere DNA-binding protein complex CBF3 subunit C

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Macromolecule #4: Suppressor of kinetochore protein 1

MacromoleculeName: Suppressor of kinetochore protein 1 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 22.35727 KDa
SequenceString: MVTSNVVLVS GEGERFTVDK KIAERSLLLK NYLNDMHDSN LQNNSDSESD SDSETNHKSK DNNNGDDDDE DDDEIVMPVP NVRSSVLQK VIEWAEHHRD SNFPDEDDDD SRKSAPVDSW DREFLKVDQE MLYEIILAAN YLNIKPLLDA GCKVVAEMIR G RSPEEIRR ...String:
MVTSNVVLVS GEGERFTVDK KIAERSLLLK NYLNDMHDSN LQNNSDSESD SDSETNHKSK DNNNGDDDDE DDDEIVMPVP NVRSSVLQK VIEWAEHHRD SNFPDEDDDD SRKSAPVDSW DREFLKVDQE MLYEIILAAN YLNIKPLLDA GCKVVAEMIR G RSPEEIRR TFNIVNDFTP EEEAAIRREN EWAEDR

UniProtKB: Suppressor of kinetochore protein 1

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Macromolecule #5: DNA (44-MER)

MacromoleculeName: DNA (44-MER) / type: dna / ID: 5 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 157.288578 KDa
SequenceString: (DA)(DT)(DC)(DA)(DG)(DC)(DG)(DC)(DC)(DA) (DA)(DA)(DC)(DA)(DA)(DT)(DA)(DT)(DG)(DG) (DA)(DA)(DA)(DA)(DT)(DC)(DC)(DA)(DC) (DA)(DG)(DA)(DA)(DA)(DG)(DC)(DT)(DA)(DT) (DT) (DC)(DA)(DT)(DT)(DG)(DA) ...String:
(DA)(DT)(DC)(DA)(DG)(DC)(DG)(DC)(DC)(DA) (DA)(DA)(DC)(DA)(DA)(DT)(DA)(DT)(DG)(DG) (DA)(DA)(DA)(DA)(DT)(DC)(DC)(DA)(DC) (DA)(DG)(DA)(DA)(DA)(DG)(DC)(DT)(DA)(DT) (DT) (DC)(DA)(DT)(DT)(DG)(DA)(DA)(DA) (DA)(DA)(DA)(DT)(DA)(DG)(DT)(DA)(DC)(DA) (DA)(DA) (DT)(DA)(DA)(DG)(DT)(DC)(DA) (DC)(DA)(DT)(DG)(DA)(DT)(DG)(DA)(DT)(DA) (DT)(DT)(DT) (DG)(DA)(DT)(DT)(DT)(DT) (DA)(DT)(DT)(DA)(DT)(DA)(DT)(DT)(DT)(DT) (DT)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DG) (DT)(DA)(DA)(DA)(DA)(DA)(DA)(DT)(DA)(DA) (DA)(DA)(DA)(DG)(DT) (DA)(DG)(DT)(DT) (DT)(DA)(DT)(DT)(DT)(DT)(DT)(DA)(DA)(DA) (DA)(DA)(DA)(DT)(DA)(DA) (DA)(DA)(DT) (DT)(DT)(DA)(DA)(DA)(DA)(DT)(DA)(DT)(DT) (DA)(DG)(DT)(DG)(DT)(DA)(DT) (DT)(DT) (DG)(DA)(DT)(DT)(DT)(DC)(DC)(DG)(DA)(DA) (DA)(DG)(DT)(DT)(DA)(DA)(DA)(DA) (DA) (DA)(DG)(DA)(DA)(DA)(DT)(DA)(DG)(DT)(DA) (DA)(DG)(DA)(DA)(DA)(DT)(DA)(DT)(DA) (DT)(DA)(DT)(DT)(DT)(DC)(DA)(DT)(DT)(DG) (DA)(DA)(DT)(DG)(DG)(DA)(DT)(DA)(DT)(DA) (DT)(DG)(DA)(DA)(DA)(DC)(DG)(DT)(DT) (DT)(DA)(DC)(DT)(DG)(DG)(DT)(DG)(DG)(DA) (DA) (DG)(DG)(DC)(DC)(DG)(DG)(DC)(DG) (DG)(DT)(DT)(DG)(DT)(DT)(DT)(DG)(DC)(DA) (DA)(DG) (DA)(DC)(DC)(DG)(DA)(DG)(DA) (DA)(DA)(DA)(DG)(DG)(DC)(DT)(DA)(DG)(DC) (DA)(DA)(DG) (DA)(DA)(DT)(DC)(DG)(DG) (DG)(DT)(DC)(DA)(DT)(DT)(DG)(DT)(DA)(DG) (DC)(DG)(DT)(DA) (DT)(DG)(DC)(DG)(DC) (DC)(DT)(DG)(DT)(DG)(DA)(DA)(DC)(DA)(DT) (DT)(DC)(DT)(DC)(DT) (DT)(DC)(DA)(DA) (DC)(DA)(DA)(DG)(DT)(DT)(DT)(DG)(DA)(DT) (DT)(DC)(DC)(DA)(DT)(DT) (DG)(DC)(DG) (DG)(DT)(DG)(DA)(DA)(DA)(DT)(DG)(DG)(DT) (DA)(DA)(DA)(DA)(DG)(DT)(DC) (DA)(DA) (DC)(DC)(DC)(DC)(DC)(DT)(DG)(DC)(DG)(DA) (DT)(DG)(DT)(DA)(DT)(DA)(DT)(DT) (DT) (DT)(DC)(DC)(DT)(DG)(DT)(DA)(DC)(DA)(DA) (DT)(DC)(DA)(DA)(DT)(DC)(DA)(DA)(DA) (DA)(DA)(DG)(DC)(DC)(DA)(DA)(DA)(DT)(DG) (DA)(DT)(DT)(DT)(DA)(DG)(DC)(DA)(DT)(DT) (DA)(DT)(DC)(DT)(DT)(DT)(DA)(DC)(DA) (DT)(DC)(DT)(DT)(DG)(DT)(DT)(DA)(DT)(DT) (DT) (DT)(DA)(DC)(DA)(DG)(DA)(DT)(DT) (DT)(DT)(DA)(DT)(DG)(DT)(DT)(DT)(DA)(DG) (DA)(DT) (DC)(DT)(DT)(DT)(DT)(DA)(DT) (DG)(DC)(DT)(DT)(DG)(DC)(DT)(DT)(DT)(DT) (DC)(DA)(DA) (DA)(DA)(DG)(DG)(DC)(DC) (DT)(DG)(DC)(DA)(DG)(DG)(DC)(DA)(DA)(DG) (DT)(DG)(DC)(DA) (DC)(DA)(DA)(DA)(DC) (DA)(DA)(DT)

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Macromolecule #6: DNA (44-MER)

MacromoleculeName: DNA (44-MER) / type: dna / ID: 6 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 156.413875 KDa
SequenceString: (DA)(DT)(DT)(DG)(DT)(DT)(DT)(DG)(DT)(DG) (DC)(DA)(DC)(DT)(DT)(DG)(DC)(DC)(DT)(DG) (DC)(DA)(DG)(DG)(DC)(DC)(DT)(DT)(DT) (DT)(DG)(DA)(DA)(DA)(DA)(DG)(DC)(DA)(DA) (DG) (DC)(DA)(DT)(DA)(DA)(DA) ...String:
(DA)(DT)(DT)(DG)(DT)(DT)(DT)(DG)(DT)(DG) (DC)(DA)(DC)(DT)(DT)(DG)(DC)(DC)(DT)(DG) (DC)(DA)(DG)(DG)(DC)(DC)(DT)(DT)(DT) (DT)(DG)(DA)(DA)(DA)(DA)(DG)(DC)(DA)(DA) (DG) (DC)(DA)(DT)(DA)(DA)(DA)(DA)(DG) (DA)(DT)(DC)(DT)(DA)(DA)(DA)(DC)(DA)(DT) (DA)(DA) (DA)(DA)(DT)(DC)(DT)(DG)(DT) (DA)(DA)(DA)(DA)(DT)(DA)(DA)(DC)(DA)(DA) (DG)(DA)(DT) (DG)(DT)(DA)(DA)(DA)(DG) (DA)(DT)(DA)(DA)(DT)(DG)(DC)(DT)(DA)(DA) (DA)(DT)(DC)(DA) (DT)(DT)(DT)(DG)(DG) (DC)(DT)(DT)(DT)(DT)(DT)(DG)(DA)(DT)(DT) (DG)(DA)(DT)(DT)(DG) (DT)(DA)(DC)(DA) (DG)(DG)(DA)(DA)(DA)(DA)(DT)(DA)(DT)(DA) (DC)(DA)(DT)(DC)(DG)(DC) (DA)(DG)(DG) (DG)(DG)(DG)(DT)(DT)(DG)(DA)(DC)(DT)(DT) (DT)(DT)(DA)(DC)(DC)(DA)(DT) (DT)(DT) (DC)(DA)(DC)(DC)(DG)(DC)(DA)(DA)(DT)(DG) (DG)(DA)(DA)(DT)(DC)(DA)(DA)(DA) (DC) (DT)(DT)(DG)(DT)(DT)(DG)(DA)(DA)(DG)(DA) (DG)(DA)(DA)(DT)(DG)(DT)(DT)(DC)(DA) (DC)(DA)(DG)(DG)(DC)(DG)(DC)(DA)(DT)(DA) (DC)(DG)(DC)(DT)(DA)(DC)(DA)(DA)(DT)(DG) (DA)(DC)(DC)(DC)(DG)(DA)(DT)(DT)(DC) (DT)(DT)(DG)(DC)(DT)(DA)(DG)(DC)(DC)(DT) (DT) (DT)(DT)(DC)(DT)(DC)(DG)(DG)(DT) (DC)(DT)(DT)(DG)(DC)(DA)(DA)(DA)(DC)(DA) (DA)(DC) (DC)(DG)(DC)(DC)(DG)(DG)(DC) (DC)(DT)(DT)(DC)(DC)(DA)(DC)(DC)(DA)(DG) (DT)(DA)(DA) (DA)(DC)(DG)(DT)(DT)(DT) (DC)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DC)(DA) (DT)(DT)(DC)(DA) (DA)(DT)(DG)(DA)(DA) (DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DT)(DT) (DC)(DT)(DT)(DA)(DC) (DT)(DA)(DT)(DT) (DT)(DC)(DT)(DT)(DT)(DT)(DT)(DT)(DA)(DA) (DC)(DT)(DT)(DT)(DC)(DG) (DG)(DA)(DA) (DA)(DT)(DC)(DA)(DA)(DA)(DT)(DA)(DC)(DA) (DC)(DT)(DA)(DA)(DT)(DA)(DT) (DT)(DT) (DT)(DA)(DA)(DA)(DT)(DT)(DT)(DT)(DA)(DT) (DT)(DT)(DT)(DT)(DT)(DA)(DA)(DA) (DA) (DA)(DT)(DA)(DA)(DA)(DC)(DT)(DA)(DC)(DT) (DT)(DT)(DT)(DT)(DA)(DT)(DT)(DT)(DT) (DT)(DT)(DA)(DC)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DA)(DA)(DA)(DA)(DA)(DT)(DA)(DT)(DA) (DA)(DT)(DA)(DA)(DA)(DA)(DT)(DC)(DA) (DA)(DA)(DT)(DA)(DT)(DC)(DA)(DT)(DC)(DA) (DT) (DG)(DT)(DG)(DA)(DC)(DT)(DT)(DA) (DT)(DT)(DT)(DG)(DT)(DA)(DC)(DT)(DA)(DT) (DT)(DT) (DT)(DT)(DT)(DC)(DA)(DA)(DT) (DG)(DA)(DA)(DT)(DA)(DG)(DC)(DT)(DT)(DT) (DC)(DT)(DG) (DT)(DG)(DG)(DA)(DT)(DT) (DT)(DT)(DC)(DC)(DA)(DT)(DA)(DT)(DT)(DG) (DT)(DT)(DT)(DG) (DG)(DC)(DG)(DC)(DT) (DG)(DA)(DT)

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Macromolecule #7: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 7 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL / In silico model: alpha fold 3 prediction
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 64688
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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