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- EMDB-74809: HCV Chiron E1E2 complexed with AR3A and AR4A Fabs -

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Open data


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Basic information

Entry
Database: EMDB / ID: EMD-74809
TitleHCV Chiron E1E2 complexed with AR3A and AR4A Fabs
Map data
Sample
  • Complex: HCV Chiron E1E2 complexed with AR3A and AR4A Fabs
KeywordsHCV vaccine / Chiron E1E2 / AR3A / AR4A / Antibody Fabs / VIRAL PROTEIN
Biological speciesHepacivirus hominis
Methodsingle particle reconstruction / negative staining / Resolution: 15.17 Å
AuthorsHung S-H / Law M
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI158193 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI168251 United States
CitationJournal: Vaccine / Year: 2025
Title: Antigenic and EM characterization of the Chiron experimental hepatitis C virus vaccine.
Authors: Shr-Hau Hung / Jonathan L Torres / Fang Chen / Erick Giang / Andrew B Ward / Mansun Law /
Abstract: The Chiron experimental HCV vaccine, the only envelope-based HCV vaccine candidate to date, was immunogenic and safe in a phase 1 trial, though its efficacy remains untested. This study examined the ...The Chiron experimental HCV vaccine, the only envelope-based HCV vaccine candidate to date, was immunogenic and safe in a phase 1 trial, though its efficacy remains untested. This study examined the antigenic properties of the vaccine antigens by immunoassays and EM, revealing inconsistent presentation of AR1/5 epitopes. The vaccine elicited mainly strain-specific neutralizing antibodies in humans and non-human primates, highlighting the need for rational antigen design to improve vaccine breadth.
History
DepositionDec 23, 2025-
Header (metadata) releaseJan 14, 2026-
Map releaseJan 14, 2026-
UpdateJan 14, 2026-
Current statusJan 14, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_74809.png

Downloads & links

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Map

FileDownload / File: emd_74809.map.gz / Format: CCP4 / Size: 67 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.93 Å
Density
Contour LevelBy AUTHOR: 0.885
Minimum - Maximum-1.3104012 - 5.8524585
Average (Standard dev.)-0.012167827 (±0.15375043)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions260260260
Spacing260260260
CellA=B=C: 501.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_74809_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_74809_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : HCV Chiron E1E2 complexed with AR3A and AR4A Fabs

EntireName: HCV Chiron E1E2 complexed with AR3A and AR4A Fabs
Components
  • Complex: HCV Chiron E1E2 complexed with AR3A and AR4A Fabs

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Supramolecule #1: HCV Chiron E1E2 complexed with AR3A and AR4A Fabs

SupramoleculeName: HCV Chiron E1E2 complexed with AR3A and AR4A Fabs / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Hepacivirus hominis

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Experimental details

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Structure determination

Methodnegative staining
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.01 mg/mL
BufferpH: 7.4
StainingType: NEGATIVE / Material: uranyl formate

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Electron microscopy

MicroscopeTFS TALOS L120C
Image recordingFilm or detector model: FEI CETA (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 120 kV / Electron source: LAB6
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Talos L120C / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 15.17 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 3607
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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