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- EMDB-74341: PSI-IsiA Complex from Anabaena 7120 -

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Basic information

Entry
Database: EMDB / ID: EMD-74341
TitlePSI-IsiA Complex from Anabaena 7120
Map dataComposite map combining focused maps with ISIA6&7, ISIA1to5, and PSI monomer masks.
Sample
  • Complex: Photosystem I monomer with 7 IsiA subunits
    • Protein or peptide: x 15 types
  • Ligand: x 11 types
KeywordsIron Stress Chlorophyll Photosystem I Complex / Photosynthesis / light harvesting
Function / homology
Function and homology information


photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosynthetic electron transport chain / photosystem I / plasma membrane-derived thylakoid membrane / chlorophyll binding / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity ...photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosynthetic electron transport chain / photosystem I / plasma membrane-derived thylakoid membrane / chlorophyll binding / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / oxidoreductase activity / magnesium ion binding / metal ion binding
Similarity search - Function
Photosystem I PsaX / Photosystem I PsaX superfamily / PsaX family / Photosystem I PsaM, reaction centre superfamily / Photosystem I PsaM, reaction centre / Photosystem I protein M (PsaM) / Photosystem antenna protein-like / Photosystem antenna protein-like superfamily / Photosystem II protein / Photosystem I reaction centre subunit VIII ...Photosystem I PsaX / Photosystem I PsaX superfamily / PsaX family / Photosystem I PsaM, reaction centre superfamily / Photosystem I PsaM, reaction centre / Photosystem I protein M (PsaM) / Photosystem antenna protein-like / Photosystem antenna protein-like superfamily / Photosystem II protein / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Photosystem I PsaD / Photosystem I, reaction centre subunit PsaD superfamily / PsaD / Photosystem I PsaE, reaction centre subunit IV / Photosystem I reaction centre subunit IV / PsaE / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I reaction centre subunit IX / PsaJ / Photosystem I PsaA / Photosystem I protein PsaC / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / : / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S dicluster domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain
Similarity search - Domain/homology
: / : / : / : / Photosystem I iron-sulfur center / Photosystem I reaction center subunit VIII / Photosystem I reaction center subunit III / Photosystem I P700 chlorophyll a apoprotein A2 1 / Photosystem I 4.8 kDa protein / Photosystem I reaction center subunit IX ...: / : / : / : / Photosystem I iron-sulfur center / Photosystem I reaction center subunit VIII / Photosystem I reaction center subunit III / Photosystem I P700 chlorophyll a apoprotein A2 1 / Photosystem I 4.8 kDa protein / Photosystem I reaction center subunit IX / Photosystem I reaction center subunit II / Photosystem I reaction center subunit IV / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit XII / Iron stress-induced chlorophyll-binding protein
Similarity search - Component
Biological speciesNostoc sp. PCC 7120 = FACHB-418 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.22 Å
AuthorsMazor YM / Maqdisi RM / Gorski CG / Pakrasi HP / Biswas SB / Niedzwiedzki DN
Funding support United States, 1 items
OrganizationGrant numberCountry
Department of Energy (DOE, United States) United States
CitationJournal: Nat Commun / Year: 2023
Title: Structure of a monomeric photosystem I core associated with iron-stress-induced-A proteins from Anabaena sp. PCC 7120.
Authors: Ryo Nagao / Koji Kato / Tasuku Hamaguchi / Yoshifumi Ueno / Naoki Tsuboshita / Shota Shimizu / Miyu Furutani / Shigeki Ehira / Yoshiki Nakajima / Keisuke Kawakami / Takehiro Suzuki / Naoshi ...Authors: Ryo Nagao / Koji Kato / Tasuku Hamaguchi / Yoshifumi Ueno / Naoki Tsuboshita / Shota Shimizu / Miyu Furutani / Shigeki Ehira / Yoshiki Nakajima / Keisuke Kawakami / Takehiro Suzuki / Naoshi Dohmae / Seiji Akimoto / Koji Yonekura / Jian-Ren Shen /
Abstract: Iron-stress-induced-A proteins (IsiAs) are expressed in cyanobacteria under iron-deficient conditions. The cyanobacterium Anabaena sp. PCC 7120 has four isiA genes; however, their binding property ...Iron-stress-induced-A proteins (IsiAs) are expressed in cyanobacteria under iron-deficient conditions. The cyanobacterium Anabaena sp. PCC 7120 has four isiA genes; however, their binding property and functional roles in PSI are still missing. We analyzed a cryo-electron microscopy structure of a PSI-IsiA supercomplex isolated from Anabaena grown under an iron-deficient condition. The PSI-IsiA structure contains six IsiA subunits associated with the PsaA side of a PSI core monomer. Three of the six IsiA subunits were identified as IsiA1 and IsiA2. The PSI-IsiA structure lacks a PsaL subunit; instead, a C-terminal domain of IsiA2 occupies the position of PsaL, which inhibits the oligomerization of PSI, leading to the formation of a PSI monomer. Furthermore, excitation-energy transfer from IsiAs to PSI appeared with a time constant of 55 ps. These findings provide insights into both the molecular assembly of the Anabaena IsiA family and the functional roles of IsiAs.
History
DepositionDec 5, 2025-
Header (metadata) releaseJun 24, 2026-
Map releaseJun 24, 2026-
UpdateJun 24, 2026-
Current statusJun 24, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_74341.png

Downloads & links

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Map

FileDownload / File: emd_74341.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite map combining focused maps with ISIA6&7, ISIA1to5, and PSI monomer masks.
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
0.63 Å/pix.
x 512 pix.
= 321.536 Å		0.63 Å/pix.
x 512 pix.
= 321.536 Å		0.63 Å/pix.
x 512 pix.
= 321.536 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.628 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 4.34
Minimum - Maximum-14.026446 - 34.680957999999997
Average (Standard dev.)0.020586325 (±1.1538727)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 321.536 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_74341_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Mask #2

Fileemd_74341_msk_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Mask #3

Fileemd_74341_msk_3.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Focused refinement map of the PSI monomer region.

Fileemd_74341_additional_1.map
AnnotationFocused refinement map of the PSI monomer region.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Global primary cryo-EM reconstruction of the complex, used...

Fileemd_74341_additional_2.map
AnnotationGlobal primary cryo-EM reconstruction of the complex, used as initial volume for subsequent local refinements.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Focused refinement map for ISIA6,7 region.

Fileemd_74341_additional_3.map
AnnotationFocused refinement map for ISIA6,7 region.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Focused refinement map for ISIA1to5 region.

Fileemd_74341_additional_4.map
AnnotationFocused refinement map for ISIA1to5 region.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Photosystem I monomer with 7 IsiA subunits

EntireName: Photosystem I monomer with 7 IsiA subunits
Components
  • Complex: Photosystem I monomer with 7 IsiA subunits
    • Protein or peptide: Photosystem I reaction center subunit XI
    • Protein or peptide: Photosystem II CP43 protein PsbC homolog
    • Protein or peptide: Photosystem II CP43 protein PsbC homolog
    • Protein or peptide: Iron stress-induced chlorophyll-binding protein
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A1
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A2 1
    • Protein or peptide: Photosystem I iron-sulfur center
    • Protein or peptide: Photosystem I reaction center subunit II
    • Protein or peptide: Photosystem I reaction center subunit IV
    • Protein or peptide: Photosystem I reaction center subunit III
    • Protein or peptide: Photosystem I reaction center subunit VIII
    • Protein or peptide: Photosystem I reaction center subunit IX
    • Protein or peptide: Photosystem I reaction center subunit PsaK
    • Protein or peptide: Photosystem I reaction center subunit XII
    • Protein or peptide: Photosystem I 4.8 kDa protein
  • Ligand: CHLOROPHYLL A
  • Ligand: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: BETA-CAROTENE
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: DODECYL-ALPHA-D-MALTOSIDE
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: PHYLLOQUINONE
  • Ligand: DODECYL-BETA-D-MALTOSIDE
  • Ligand: CHLOROPHYLL A ISOMER
  • Ligand: beta,beta-caroten-4-one
  • Ligand: IRON/SULFUR CLUSTER

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Supramolecule #1: Photosystem I monomer with 7 IsiA subunits

SupramoleculeName: Photosystem I monomer with 7 IsiA subunits / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#15
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)

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Macromolecule #1: Photosystem I reaction center subunit XI

MacromoleculeName: Photosystem I reaction center subunit XI / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 51.717449 KDa
SequenceString: MTTATINPQQ YGWWAGNARF INLSGRLLGA HIAHAGLIIL WAGAMTLFEI TKYNPSLPIY EQGLILLPHL ATLGFGIGDG GQIIDTYPY FVIGVVHLVS SAVLAAGGIY HALLGPEVLP ENNQFPGFFG YDWEDEDKMT TIIGIHLLLL GAGAWLLVAK A LFWGGLYD ...String:
MTTATINPQQ YGWWAGNARF INLSGRLLGA HIAHAGLIIL WAGAMTLFEI TKYNPSLPIY EQGLILLPHL ATLGFGIGDG GQIIDTYPY FVIGVVHLVS SAVLAAGGIY HALLGPEVLP ENNQFPGFFG YDWEDEDKMT TIIGIHLLLL GAGAWLLVAK A LFWGGLYD STVASVRVIT EPTVNPARIF GYLFGAFGKQ GMAAVNNLED VVGGHIWVGI LCIGGGFWHI LTQPFAWAKK VL FWSGEAY LSYSLAALAY MGLLAAYFVT VNDTVYPTEF YGPLGFSSTS GVISVRTWLA TSHFALAIVF LSGHIWHALR VRV LEAGLN FEQGVVNYLD TPELGNLQTP INTSDLTLKF LVNLPIYRPG LSAFARGLEI GMAHGYFLLG PFVKLGPLRN TEFA NQAGL LATIGLLLIL SICLWLYGSA WFQEGKSPQG ELPENLKTAK SWSEFNAGWI VGSCGGALFA YLLVTNSSLF F

UniProtKB: UNIPROTKB: A0A1Z4KKX8

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Macromolecule #2: Photosystem II CP43 protein PsbC homolog

MacromoleculeName: Photosystem II CP43 protein PsbC homolog / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 37.540383 KDa
SequenceString: MTSIAVQTPN SIPWWSGNAR LTHLSGRLLG AHVAHAGLIV LWAGATTLFE LARFQTSQPM YEQGLILLPH LAVLGWGVGS GGQVIDTYP YFVIGVLHLI SSAFLGLGGI FHALLGPAIL EERFSFFGYR WQDANKMTTI LGIHLVLLGL GAGLLVAKAV F FGGLYDPT ...String:
MTSIAVQTPN SIPWWSGNAR LTHLSGRLLG AHVAHAGLIV LWAGATTLFE LARFQTSQPM YEQGLILLPH LAVLGWGVGS GGQVIDTYP YFVIGVLHLI SSAFLGLGGI FHALLGPAIL EERFSFFGYR WQDANKMTTI LGIHLVLLGL GAGLLVAKAV F FGGLYDPT LGEVRVISHP TWNFATIFSY LFGSHKYWIA GVDNLEDLVG GHIWVSILCI AGGLWHITTE PSAFVKSLFV WS GEAYLSY SLGALSLMSL IAAYFVSINT LAYPVEFFGP ALSIGFDRFP YFQSADLLSS RVWLANAHFW LGFFFLQGHL WHA LRAAGF DFRTGKVLLP TRGQVI

UniProtKB: UNIPROTKB: A0A1Z4KKX9

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Macromolecule #3: Photosystem II CP43 protein PsbC homolog

MacromoleculeName: Photosystem II CP43 protein PsbC homolog / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 35.161734 KDa
SequenceString: MAISTDRTFA ANTQLPWLIG NARLTDLSGQ LLGAHIAHAG LIMFWAGTTT ISEVLRFVPD VPMYEQGLIL LPHLATLGWG VGVGGQVID TYPYFVIGVL HLIASAVLGA GGLFHVFRTP AILYNNGGRT AQFHYEWNDP KQLSLILGHH LIILGLGAFL L VLKAMFFG ...String:
MAISTDRTFA ANTQLPWLIG NARLTDLSGQ LLGAHIAHAG LIMFWAGTTT ISEVLRFVPD VPMYEQGLIL LPHLATLGWG VGVGGQVID TYPYFVIGVL HLIASAVLGA GGLFHVFRTP AILYNNGGRT AQFHYEWNDP KQLSLILGHH LIILGLGAFL L VLKAMFFG GIYDTNLGDV RSITNPTLNP GIIFGYLVGL KDHTWHPLGI ATVDNLEDVI GGHIWIGGIL IFGGIWHIIV QP FAWVRRI LPIENGEEIL AYSLLGLALM AWISASFVGY NTTVFPTEFY GSERLFAANI QFFLGILAFL GYVWHAWRGR NIS

UniProtKB: UNIPROTKB: A0A1Z4KKX7

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Macromolecule #4: Iron stress-induced chlorophyll-binding protein

MacromoleculeName: Iron stress-induced chlorophyll-binding protein / type: protein_or_peptide / ID: 4 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 37.501277 KDa
SequenceString: MQTYDNPNIK YDWWAGNARF ANLSGLFIGA HVAQAALTTL WAGAFTWFEI SRYKPEIPMG EQGLILLPHL ATLGFGVGVS GQVVNTYPY FVIGALHLIS SAVLGAGALF HTFKGPRNLK NTTGSARKFH FEWNDPKQLG LILGHHLLFL GMAALLLVGK A MFWGGLYD ...String:
MQTYDNPNIK YDWWAGNARF ANLSGLFIGA HVAQAALTTL WAGAFTWFEI SRYKPEIPMG EQGLILLPHL ATLGFGVGVS GQVVNTYPY FVIGALHLIS SAVLGAGALF HTFKGPRNLK NTTGSARKFH FEWNDPKQLG LILGHHLLFL GMAALLLVGK A MFWGGLYD ATTQVVRVVN HPTLNPFVIY GYQTHFASVN NLEDLVGGHI YVGLILIGGG IWHIVKEPLP WAKKLLIFSG EA ILSYSLG GIALAGFVAA YFCAVNTLAY PVEFYGAPLE LKFGVTPYFA DTVKLADGGY SARAWLANAH FFLAFFFLQG HLW HALRAI GVDFRQIEKS LNAISS

UniProtKB: Iron stress-induced chlorophyll-binding protein

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Macromolecule #5: Photosystem I P700 chlorophyll a apoprotein A1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 83.28968 KDa
SequenceString: MTISPPEREE KKARVIVDKD PVPTSFEKWA QPGHFDRTLA RGPKTTTWIW NLHALAHDFD THTSDLEDIS RKIFAAHFGH LAVVTIWLS GMIFHGAKFS NYEAWLSDPL NVRPSAQVVW PIVGQDILNG DVGGGFHGIQ ITSGLFQVWR GWGITNSFQL Y CTAIGGLV ...String:
MTISPPEREE KKARVIVDKD PVPTSFEKWA QPGHFDRTLA RGPKTTTWIW NLHALAHDFD THTSDLEDIS RKIFAAHFGH LAVVTIWLS GMIFHGAKFS NYEAWLSDPL NVRPSAQVVW PIVGQDILNG DVGGGFHGIQ ITSGLFQVWR GWGITNSFQL Y CTAIGGLV LAGLFLFAGW FHYHKRAPKL EWFQNVESML NHHLQVLLGC GSLGWAGHLI HVSAPINKLM DAGVAVKDIP LP HEFILNK SLLIDLFPGF AAGLTPFFTL NWGQYADFLT FKGGLNPVTG GLWMTDIAHH HLAIAVVFII AGHQYRTNWG IGH SIKEIL ENHKGPFTGE GHKGLYENLT TSWHAQLATN LAFLGSLTII IAHHMYAMPP YPYLATDYAT QLCIFTHHIW IGGF LIVGG AAHAAIFMVR DYDPVVNQNN VLDRVIRHRD AIISHLNWVC IFLGFHSFGL YIHNDTMRAL GRPQDMFSDT AIQLQ PVFA QWVQNLHTLA PGGTAPNALE PVSYAFGGGV LAVGGKVAMM PIALGTADFL IHHIHAFTIH VTVLILLKGV LFARSS RLI PDKANLGFRF PCDGPGRGGT CQVSGWDHVF LGLFWMYNSL SIVIFHFSWK MQSDVWGTVD AAGNVSHITG GNFAQSA IT INGWLRDFLW AQASQVINSY GSALSAYGLM FLGAHFVWAF SLMFLFSGRG YWQELIESIV WAHNKLKVAP AIQPRALS I TQGRAVGVAH YLLGGIATTW AFFHAHILSV G

UniProtKB: Photosystem I P700 chlorophyll a apoprotein A1

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Macromolecule #6: Photosystem I P700 chlorophyll a apoprotein A2 1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A2 1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 83.399961 KDa
SequenceString: MATKFPKFSQ DLAQDPTTRR IWYAMAMGND FESHDGMTEE NLYQKIFATH FGHLAIIFLW ASSLLFHVAW QGNFEQWIKD PLHVRPIAH AIWDPHFGKP AIEAFTQAGA NGPVNIAYSG VYHWWYTIGM RTNTELYTGS VFLLLFASLF LFAGWLHLQP K FRPSLAWF ...String:
MATKFPKFSQ DLAQDPTTRR IWYAMAMGND FESHDGMTEE NLYQKIFATH FGHLAIIFLW ASSLLFHVAW QGNFEQWIKD PLHVRPIAH AIWDPHFGKP AIEAFTQAGA NGPVNIAYSG VYHWWYTIGM RTNTELYTGS VFLLLFASLF LFAGWLHLQP K FRPSLAWF KSAESRLNHH LAGLFGVSSL AWAGHLIHVA IPESRGQHVG WDNFLSTAPH PAGLQPFFTG NWGVYAQNPD TA GHIFSTS QGAGTAILTF LGGFHPQTES LWLTDMAHHH LAIAVLFIVA GHMYRTNFGI GHSIKEMMNA KTFFGKPVEG PFN MPHQGI YDTYNNSLHF QLGWHLACLG VVTSWVAQHM YSLPSYAFIA KDYTTQAALY THHQYIAIFL MVGAFAHGAI FLVR DYDPE QNKGNVLERV LQHKEAIISH LSWVSLFLGF HTLGLYVHND VVVAFGTPEK QILIEPVFAQ FIQAAHGKVL YGLDT LLSN PDSVAYTAYP NYANVWLPGW LDAINSGTNS LFLTIGPGDF LVHHAIALGL HTTTLILVKG ALDARGSKLM PDKKDF GYA FPCDGPGRGG TCDISAWDSF YLSLFWALNT VGWVTFYWHW KHLGIWQGNV AQFNENSTYL MGWFRDYLWA NSAQLIN GY NPYGVNNLSV WAWMFLFGHL VWATGFMFLI SWRGYWQELI ETLVWAHERT PIANLVRWKD KPVALSIVQA RVVGLAHF T VGYVLTYAAF LIASTAGKF

UniProtKB: Photosystem I P700 chlorophyll a apoprotein A2 1

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Macromolecule #7: Photosystem I iron-sulfur center

MacromoleculeName: Photosystem I iron-sulfur center / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 8.825206 KDa
SequenceString:
MSHTVKIYDT CIGCTQCVRA CPTDVLEMVP WDGCKAAQVA SSPRTEDCVG CKRCETACPT DFLSIRVYLG AETTRSMGLA Y

UniProtKB: Photosystem I iron-sulfur center

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Macromolecule #8: Photosystem I reaction center subunit II

MacromoleculeName: Photosystem I reaction center subunit II / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 15.17624 KDa
SequenceString:
MAETLSGKTP LFAGSTGGLL TKAVEEEKYA ITWTSPKAQV FELPTGGAAT MHEGENLLYI ARKEYGIALG GQLRKFKITN YKIYRILPS GETTFIHPAD GVFPEKVNAG REKVRFNARS IGENPNPSQV KFSGKATYDA

UniProtKB: Photosystem I reaction center subunit II

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Macromolecule #9: Photosystem I reaction center subunit IV

MacromoleculeName: Photosystem I reaction center subunit IV / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 7.897051 KDa
SequenceString:
MVQRGSKVRI LRPESYWFQD VGTVASVDQS GIKYPVIVRF DKVNYAGINT NNFAVDELIE VEAPKAKAKK

UniProtKB: Photosystem I reaction center subunit IV

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Macromolecule #10: Photosystem I reaction center subunit III

MacromoleculeName: Photosystem I reaction center subunit III / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 17.850568 KDa
SequenceString:
MRRLFALILV ICLSFSFAPP AKALGADLTP CAENPAFQAL AKNARNTTAD PQSGQKRFER YSQALCGPEG YPHLIVDGRL DRAGDFLIP SILFLYIAGW IGWVGRAYLQ AIKKDSDTEQ KEIQLDLGIA LPIIATGFAW PAAAVKELLS GELTAKDSEI T VSPR

UniProtKB: Photosystem I reaction center subunit III

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Macromolecule #11: Photosystem I reaction center subunit VIII

MacromoleculeName: Photosystem I reaction center subunit VIII / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 5.066925 KDa
SequenceString:
MATAFLPSIL ADASFLSSIF VPVIGWVVPI ATFSFLFLYI EREDVA

UniProtKB: Photosystem I reaction center subunit VIII

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Macromolecule #12: Photosystem I reaction center subunit IX

MacromoleculeName: Photosystem I reaction center subunit IX / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 5.499422 KDa
SequenceString:
MADKADQSSY LIKFISTAPV AATIWLTITA GILIEFNRFF PDLLFHPLP

UniProtKB: Photosystem I reaction center subunit IX

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Macromolecule #13: Photosystem I reaction center subunit PsaK

MacromoleculeName: Photosystem I reaction center subunit PsaK / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 8.560202 KDa
SequenceString:
MISSTLLAVA ATVPATPEWS PTVGIIISAS SVVALLFALK VEYPQVGPKF PGLPISVPTF IGAMAFGHVL GIGIVLGLTN IGRL

UniProtKB: UNIPROTKB: A0ABR6S7R8

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Macromolecule #14: Photosystem I reaction center subunit XII

MacromoleculeName: Photosystem I reaction center subunit XII / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 3.538204 KDa
SequenceString:
MSSISDTQVY IALVVALIPG LLAWRLATEL YK

UniProtKB: Photosystem I reaction center subunit XII

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Macromolecule #15: Photosystem I 4.8 kDa protein

MacromoleculeName: Photosystem I 4.8 kDa protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Molecular weightTheoretical: 4.872792 KDa
SequenceString:
MAKAKISPVA NTGAKPPYTF RTGWALLLLA VNFLVAAYYF HIIQ

UniProtKB: Photosystem I 4.8 kDa protein

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Macromolecule #16: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 16 / Number of copies: 204 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A

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Macromolecule #17: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

MacromoleculeName: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 17 / Number of copies: 1 / Formula: LUT
Molecular weightTheoretical: 568.871 Da
Chemical component information

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

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Macromolecule #18: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 18 / Number of copies: 43 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE

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Macromolecule #19: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 19 / Number of copies: 4 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #20: DODECYL-ALPHA-D-MALTOSIDE

MacromoleculeName: DODECYL-ALPHA-D-MALTOSIDE / type: ligand / ID: 20 / Number of copies: 2 / Formula: LMU
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

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Macromolecule #21: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 21 / Number of copies: 7 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

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Macromolecule #22: PHYLLOQUINONE

MacromoleculeName: PHYLLOQUINONE / type: ligand / ID: 22 / Number of copies: 2 / Formula: PQN
Molecular weightTheoretical: 450.696 Da
Chemical component information

ChemComp-PQN:
PHYLLOQUINONE

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Macromolecule #23: DODECYL-BETA-D-MALTOSIDE

MacromoleculeName: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 23 / Number of copies: 4 / Formula: LMT
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

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Macromolecule #24: CHLOROPHYLL A ISOMER

MacromoleculeName: CHLOROPHYLL A ISOMER / type: ligand / ID: 24 / Number of copies: 1 / Formula: CL0
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CL0:
CHLOROPHYLL A ISOMER

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Macromolecule #25: beta,beta-caroten-4-one

MacromoleculeName: beta,beta-caroten-4-one / type: ligand / ID: 25 / Number of copies: 3 / Formula: ECH
Molecular weightTheoretical: 550.856 Da
Chemical component information

ChemComp-ECH:
beta,beta-caroten-4-one

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Macromolecule #26: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 26 / Number of copies: 3 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 35.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

7y3f

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.22 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 96842
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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