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- EMDB-73265: Fab-14/SARS-CoV-2 D614G spike complex, Mode II, subgroup II confo... -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-73265
TitleFab-14/SARS-CoV-2 D614G spike complex, Mode II, subgroup II conformation
Map data
Sample
  • Complex: Fab-14/SARS-CoV-2 D614G spike complex
KeywordsSARS-CoV-2 / neutralizing antibody / VIRAL PROTEIN
Biological speciesSevere acute respiratory syndrome coronavirus 2
Methodsingle particle reconstruction / cryo EM / Resolution: 3.49 Å
AuthorsWang Y / Hu Y / Leiman P / Xie X
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM139034 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM158090 United States
CitationJournal: To Be Published
Title: Neutralization of SARS-CoV-2 by IgM-14 via Engagement of Two Distinct Spike Epitopes
Authors: Wang Y / Hu Y / Ku Z / Yeung J / Zou J / Woodson M / Prokhorov N / Knyazhanskaya E / Zhao H / Sherman M / An Z / Carroll S / Shi P-Y / Leiman P / Xie X
History
DepositionOct 13, 2025-
Header (metadata) releaseMar 18, 2026-
Map releaseMar 18, 2026-
UpdateMar 18, 2026-
Current statusMar 18, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_73265.png

Downloads & links

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Map

FileDownload / File: emd_73265.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.84 Å/pix.
x 420 pix.
= 352.338 Å		0.84 Å/pix.
x 420 pix.
= 352.338 Å		0.84 Å/pix.
x 420 pix.
= 352.338 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.8389 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.004
Minimum - Maximum-0.011324043 - 0.02645911
Average (Standard dev.)0.0000613444 (±0.0006315046)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 352.338 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: half map B

Fileemd_73265_half_map_1.map
Annotationhalf map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map A

Fileemd_73265_half_map_2.map
Annotationhalf map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Fab-14/SARS-CoV-2 D614G spike complex

EntireName: Fab-14/SARS-CoV-2 D614G spike complex
Components
  • Complex: Fab-14/SARS-CoV-2 D614G spike complex

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Supramolecule #1: Fab-14/SARS-CoV-2 D614G spike complex

SupramoleculeName: Fab-14/SARS-CoV-2 D614G spike complex / type: complex / ID: 1 / Parent: 0 / Details: Mode I, subgroup II, 1-Fab-14 bind to 1-up-RBD
Source (natural)Organism: Severe acute respiratory syndrome coronavirus 2

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.3 mg/mL
BufferpH: 8
GridModel: Quantifoil R2/2 / Material: COPPER / Pretreatment - Type: PLASMA CLEANING
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 295 K

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 43.7 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: DIFFRACTION / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 10500
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.49 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 45209
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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