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- EMDB-72977: Octopus sensory receptor CRT1 bound to Progesterone -

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Basic information

Entry
Database: EMDB / ID: EMD-72977
TitleOctopus sensory receptor CRT1 bound to Progesterone
Map data
Sample
  • Complex: octopus chemotactile receptor CRT1 bound to progesterone
    • Protein or peptide: Chemotactile receptor CRT1
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
  • Ligand: PROGESTERONE
Keywordscomplex / membrane protein
Function / homology
Function and homology information


extracellular ligand-gated monoatomic ion channel activity / transmembrane signaling receptor activity / membrane
Similarity search - Function
Neurotransmitter-gated ion-channel transmembrane domain / Neurotransmitter-gated ion-channel transmembrane region / Neurotransmitter-gated ion-channel transmembrane domain superfamily / Neuronal acetylcholine receptor / Neurotransmitter-gated ion-channel / Neurotransmitter-gated ion-channel ligand-binding domain / Neurotransmitter-gated ion-channel ligand-binding domain superfamily / Neurotransmitter-gated ion-channel ligand binding domain
Similarity search - Domain/homology
Chemotactile receptor for terpenes 1
Similarity search - Component
Biological speciesOctopus bimaculoides (California two-spot octopus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsJiang H / Hibbs RE
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS) United States
CitationJournal: Science / Year: 2026
Title: A sensory system for mating in octopus.
Authors: Pablo S Villar / Hao Jiang / Tatiana Shugaeva / Emma L Berdan / Arpita Kulkarni / Makoto Hiroi / Giovanni Masucci / Sam Reiter / Erik Lindahl / Rebecca J Howard / Ryan E Hibbs / Nicholas W Bellono /
Abstract: Sensory systems for mate recognition maintain species boundaries and influence diversification. Thus, uncovering how molecules and receptors evolve to mediate this critical function is essential to ...Sensory systems for mate recognition maintain species boundaries and influence diversification. Thus, uncovering how molecules and receptors evolve to mediate this critical function is essential to understanding biodiversity. Male octopuses use a specialized arm called the hectocotylus to identify females and navigate their internal organs to reach the oviduct and deliver sperm. Here, we discovered that the hectocotylus is a dual sensory and mating organ that uses contact-dependent chemosensation of progesterone, a conserved ovarian hormone. We identified chemotactile receptors for progesterone and resolved the structural basis for their evolution from ancestral neurotransmitter receptors and subsequent expansion and tuning across cephalopods. These findings reveal principles by which sensory innovations shape reproductive behavior and suggest mechanisms for how sensory evolution contributes to the diversification of life.
History
DepositionOct 1, 2025-
Header (metadata) releaseApr 1, 2026-
Map releaseApr 1, 2026-
UpdateMay 6, 2026-
Current statusMay 6, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_72977.png

Downloads & links

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Map

FileDownload / File: emd_72977.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 320 pix.
= 297.6 Å		0.93 Å/pix.
x 320 pix.
= 297.6 Å		0.93 Å/pix.
x 320 pix.
= 297.6 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.93 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.11
Minimum - Maximum-0.21168788 - 0.611191
Average (Standard dev.)0.0012126708 (±0.015254051)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 297.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_72977_msk_1.map
Projections & Slices
AxesZYX

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Half map: #1

Fileemd_72977_half_map_1.map
Projections & Slices
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Histogram

Histogram (log scale)

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Half map: #2

Fileemd_72977_half_map_2.map
Projections & Slices
AxesZYX

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Sample components

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Entire : octopus chemotactile receptor CRT1 bound to progesterone

EntireName: octopus chemotactile receptor CRT1 bound to progesterone
Components
  • Complex: octopus chemotactile receptor CRT1 bound to progesterone
    • Protein or peptide: Chemotactile receptor CRT1
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
  • Ligand: PROGESTERONE

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Supramolecule #1: octopus chemotactile receptor CRT1 bound to progesterone

SupramoleculeName: octopus chemotactile receptor CRT1 bound to progesterone
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Octopus bimaculoides (California two-spot octopus)

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Macromolecule #1: Chemotactile receptor CRT1

MacromoleculeName: Chemotactile receptor CRT1 / type: protein_or_peptide / ID: 1 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Octopus bimaculoides (California two-spot octopus)
Molecular weightTheoretical: 47.334262 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MIFVIYLVFI LISSIPIVKS TPTYGDERLL REKLLTNYSK SIRPVINLTK VVDVTALLYL QTLYDLDFVN NFIMARYYLG LIWIDEKLT WNPLDYNNIT SIYLPKDKIW TPPIKMCNSM DKSEENDGVG ELMLTYTGWI NMWSFRLLHT YCQINAYTYP F DEHTCEIY ...String:
MIFVIYLVFI LISSIPIVKS TPTYGDERLL REKLLTNYSK SIRPVINLTK VVDVTALLYL QTLYDLDFVN NFIMARYYLG LIWIDEKLT WNPLDYNNIT SIYLPKDKIW TPPIKMCNSM DKSEENDGVG ELMLTYTGWI NMWSFRLLHT YCQINAYTYP F DEHTCEIY LCVALHTINH TRIKELIYED SKFTQNYKWD INVSGKVNGT DELFSYAFAP MYLRRKLTVG IIAMLIPTVM MT ILTIFVF LLPPESGEKV SLATTIFLSN VLYLVQIDKT TPTNTKYPSL LMLYLMLLSM LSGIATLGSV VISKLYVIQS PSL RKSNPS DQNMNKSHTN KVADISTISK VQSDLPIREK PNEKRIYCIS DYIRLDDIFL KLSIATSVII SMIFTCLLFI PLES SAWSH PQFEK

UniProtKB: Chemotactile receptor for terpenes 1

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Macromolecule #2: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 2 / Number of copies: 25 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Macromolecule #3: PROGESTERONE

MacromoleculeName: PROGESTERONE / type: ligand / ID: 3 / Number of copies: 5 / Formula: STR
Molecular weightTheoretical: 314.462 Da
Chemical component information

ChemComp-STR:
PROGESTERONE / hormone*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

8eis

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 241406
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

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