EMDB-7283: Single-Molecule 3D Image of DNA Origami Bennett Linkage by Indivi...

基本情報

• 登録情報: データベース: EMDB / ID: EMD-7283
• タイトル: Single-Molecule 3D Image of DNA Origami Bennett Linkage by Individual Particle Electron Tomography (No. 037)
• マップデータ: Origami Bennett Linkage (No. 037)

試料

• 複合体: DNA origami Bennett linkage
  • 複合体: M13 phage genome segment
  • 複合体: Synthetic DNA oligonucleotides

• 生物種: Escherichia virus M13 (ウイルス) / synthetic construct (人工物)
• 手法: 電子線トモグラフィー法 / クライオ電子顕微鏡法 / ネガティブ染色法 / 解像度: 108.6 Å
• データ登録者: Lei D / Marras A / Liu J / Huang C / Zhou L / Castro C / Su H / Ren G

資金援助

米国, 4件

Organization	Grant number	国
National Science Foundation (NSF, United States)	DMR-1344290	米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R01GM104427	米国
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)	R01HL115153	米国
Department of Energy (DOE, United States)	DE-AC02-05CH11231	米国

• 引用: ジャーナル: Nat Commun / 年: 2018; タイトル: Three-dimensional structural dynamics of DNA origami Bennett linkages using individual-particle electron tomography.; 著者: Dongsheng Lei / Alexander E Marras / Jianfang Liu / Chao-Min Huang / Lifeng Zhou / Carlos E Castro / Hai-Jun Su / Gang Ren / ; 要旨: Scaffolded DNA origami has proven to be a powerful and efficient technique to fabricate functional nanomachines by programming the folding of a single-stranded DNA template strand into three-dimensional (3D) nanostructures, designed to be precisely motion-controlled. Although two-dimensional (2D) imaging of DNA nanomachines using transmission electron microscopy and atomic force microscopy suggested these nanomachines are dynamic in 3D, geometric analysis based on 2D imaging was insufficient to uncover the exact motion in 3D. Here we use the individual-particle electron tomography method and reconstruct 129 density maps from 129 individual DNA origami Bennett linkage mechanisms at ~ 6-14 nm resolution. The statistical analyses of these conformations lead to understanding the 3D structural dynamics of Bennett linkage mechanisms. Moreover, our effort provides experimental verification of a theoretical kinematics model of DNA origami, which can be used as feedback to improve the design and control of motion via optimized DNA sequences and routing.

履歴

登録	2017年12月6日	-
ヘッダ(付随情報) 公開	2018年1月10日	-
マップ公開	2018年2月21日	-
更新	2019年12月25日	-
現状	2019年12月25日	処理サイト: RCSB / 状態: 公開