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- EMDB-71729: PhuZ Tubulin from phage Goslar (locally refined monomer) -

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Basic information

Entry
Database: EMDB / ID: EMD-71729
TitlePhuZ Tubulin from phage Goslar (locally refined monomer)
Map data
Sample
  • Organelle or cellular component: Tubulin PhuZ from Bacteriophage Goslar
    • Protein or peptide: Tubulin/FtsZ GTPase domain-containing protein
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
KeywordsPhage / Tubulin / PhuZ / VIRAL PROTEIN
Function / homologyTubulin/FtsZ, GTPase domain superfamily / Tubulin/FtsZ GTPase domain-containing protein
Function and homology information
Biological speciesGoslarvirus
Methodhelical reconstruction / cryo EM / Resolution: 2.75 Å
AuthorsBasu D / Gu Y / Corbett KD
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI)Emerging Pathogens Initiative United States
CitationJournal: To Be Published
Title: PhuZ Tubulin from phage Goslar (locally refined monomer)
Authors: Basu D / Gu Y / Corbett KD
History
DepositionJul 16, 2025-
Header (metadata) releaseDec 24, 2025-
Map releaseDec 24, 2025-
UpdateDec 24, 2025-
Current statusDec 24, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_71729.png

Downloads & links

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Map

FileDownload / File: emd_71729.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 400 pix.
= 328.8 Å		0.82 Å/pix.
x 400 pix.
= 328.8 Å		0.82 Å/pix.
x 400 pix.
= 328.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.822 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.12
Minimum - Maximum-0.3478667 - 0.7707536
Average (Standard dev.)0.0029601038 (±0.017432993)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 328.80002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_71729_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_71729_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Tubulin PhuZ from Bacteriophage Goslar

EntireName: Tubulin PhuZ from Bacteriophage Goslar
Components
  • Organelle or cellular component: Tubulin PhuZ from Bacteriophage Goslar
    • Protein or peptide: Tubulin/FtsZ GTPase domain-containing protein
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

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Supramolecule #1: Tubulin PhuZ from Bacteriophage Goslar

SupramoleculeName: Tubulin PhuZ from Bacteriophage Goslar / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Goslarvirus

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Macromolecule #1: Tubulin/FtsZ GTPase domain-containing protein

MacromoleculeName: Tubulin/FtsZ GTPase domain-containing protein / type: protein_or_peptide / ID: 1 / Details: Codon 1 deleted; N-terminal SNA tag scar / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 35.662961 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: SNAQENQVHI WAVGGCGGEI GSAFEGEVAK GLHSMTYVDT SRANLRDSTL GDDHIYIFKD AAGRELDGSG KKRDENAELI CSQIPGILN KHTPGKFNIV VFSLSGGSGS VAGPALVANL LERGERVVCC IVASFDDERQ TINADRTFKG LMAAAEGLKR P VIVSYHMN ...String:
SNAQENQVHI WAVGGCGGEI GSAFEGEVAK GLHSMTYVDT SRANLRDSTL GDDHIYIFKD AAGRELDGSG KKRDENAELI CSQIPGILN KHTPGKFNIV VFSLSGGSGS VAGPALVANL LERGERVVCC IVASFDDERQ TINADRTFKG LMAAAEGLKR P VIVSYHMN GENGKSEQDV NLDVQVVISQ LCVLFSGENR RLDSADLENF LNHPRVTGLE PTITELNTFM GYEWTKENDS PI AFCTLYP KDATKVMGVS AIYSCDGFVP ESSPIREATS FVLSTDRLGG IIKQIADEAK RYIEHRNARQ QAPKVGDETV AEA KNSGFL LF

UniProtKB: Tubulin/FtsZ GTPase domain-containing protein

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Macromolecule #2: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

MacromoleculeName: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 2 / Number of copies: 1 / Formula: G2P
Molecular weightTheoretical: 521.208 Da
Chemical component information

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

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Experimental details

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Structure determination

Methodcryo EM
Processinghelical reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 55.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Helical parameters - Δz: 4.883 Å
Applied symmetry - Helical parameters - Δ&Phi: -79.906 °
Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric)
Resolution.type: BY AUTHOR / Resolution: 2.75 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 396117
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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