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TitleA bacteriophage tubulin forms microtubule-like assemblies with nine protofilaments.
Journal, issue, pagesStructure, Vol. 34, Page 394-403.e3, Year 2026
Publish dateJul 17, 2025
AuthorsDwaipayan Basu / Siyu Chen / Ying-Xing Li / Niklas Klusch / Koe Inlow / Joe Pogliano / Elizabeth Villa / Kevin D Corbett /
PubMed AbstractTubulin family proteins play central roles in the organization and dynamics of cytoskeletal systems across the Tree of Life. In one family of bacteriophages (phages), tubulin-like proteins called ...Tubulin family proteins play central roles in the organization and dynamics of cytoskeletal systems across the Tree of Life. In one family of bacteriophages (phages), tubulin-like proteins called PhuZ (Phage tubulin/FtsZ) form dynamic filaments that position and rotate an intracellular compartment, the "phage nucleus," in which the phage genome is replicated. PhuZ filaments also mediate trafficking of nascent capsids from the cell periphery to the phage nucleus for genome packaging. PhuZ from the Pseudomonas-infecting phages 201Phi2-1 and PhiKZ form assemblies with three protofilaments. Here, we determine a 2.8 Å resolution structure of PhuZ from the E. coli-infecting phage Goslar, which forms an elaborate "cytoskeletal vortex" in infected cells. We find that in vitro-assembled Goslar PhuZ forms rigid tubes with nine nearly-straight protofilaments. The lateral interactions mediating this assembly are fundamentally different from eukaryotic tubulin, leading to a distinctive overall architecture for Goslar PhuZ filaments.
External linksStructure / PubMed:41468894 / PubMed Central
MethodsEM (helical sym.)
Resolution2.75 - 2.84 Å
Structure data

71729

9pm9

EMDB-71729, PDB-9pm9:
PhuZ Tubulin from phage Goslar (locally refined monomer)
Method: EM (helical sym.) / Resolution: 2.75 Å

71738

9pmk

EMDB-71738: PhuZ Tubulin Filament from phage Goslar
PDB-9pmk: PhuZ Tubulin Tetramer from phage Goslar
Method: EM (helical sym.) / Resolution: 2.84 Å

Chemicals

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

Source
  • goslarvirus
KeywordsVIRAL PROTEIN / Phage / Tubulin / PhuZ

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