EMDB-71645: In vitro reconstituted complex of purified S. pombe large ribosom...

Basic information

Entry

Database: EMDB / ID: EMD-71645

• Title: In vitro reconstituted complex of purified S. pombe large ribosomal subunit and SNOR
• Map data: in vitro reconstituted complex of purified S. pombe large ribosomal subunit and SNOR

Sample

• Complex: In vitro reconstituted complex of purified S. pombe large ribosomal subunit and SNOR
  • Protein or peptide: x 41 types
  • RNA: x 3 types
• Ligand: x 1 types

• Keywords: 60S / RIBOSOME / large subunit / SNOR / pombe / Yeast / Hibernating / Glucose

Function / homology

Function:

ribonuclease MRP complex / L13a-mediated translational silencing of Ceruloplasmin expression / Formation of a pool of free 40S subunits / GTP hydrolysis and joining of the 60S ribosomal subunit / SRP-dependent cotranslational protein targeting to membrane / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / preribosome / tRNA processing / cytosolic large ribosomal subunit assembly / pre-mRNA intronic binding / protein-RNA complex assembly / maturation of LSU-rRNA / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA processing / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / negative regulation of gene expression / mRNA binding / nucleolus / RNA binding / zinc ion binding / nucleus / cytosol

Domain/homology:

Ribosome maturation protein Sdo1/SBDS-like / Ribosome maturation protein SDO1/SBDS, N-terminal / Ribosome maturation protein SBDS, N-terminal domain superfamily / Shwachman-Bodian-Diamond syndrome (SBDS) N-terminal domain / : / Ribosomal protein L28e / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L19, eukaryotic / Ribosomal protein L10e, conserved site / : / Ribosomal protein L10e signature. / Ribosomal protein L6e signature. / Ribosomal protein L44e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L10e / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / : / Ribosomal protein L30e signature 1. / Ribosomal protein L36e signature. / Ribosomal protein L44e / Ribosomal protein L44 / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L35Ae, conserved site / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L35Ae signature. / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / : / Ribosomal Protein L6, KOW domain / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / 60S ribosomal protein L35 / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein L7A/L8 / : / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L6e / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / 60S ribosomal protein L6E / : / Ribosomal protein L30/YlxQ / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L18e / Ribosomal protein L31e, conserved site / Ribosomal protein L31e signature. / Ribosomal protein L14e domain / Ribosomal protein L14 / : / : / Ribosomal protein L19e, C-terminal domain / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e, N-terminal domain / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L37ae / Ribosomal L37ae protein family

Component:

Large ribosomal subunit protein eL8 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL19B / Large ribosomal subunit protein eL21B / Large ribosomal subunit protein uL13A / Large ribosomal subunit protein uL30C / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL18B / Large ribosomal subunit protein eL24B / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL6B / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein eL36B / Large ribosomal subunit protein eL37B / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL14B / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein uL2C / Large ribosomal subunit protein uL5A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein uL3A / Large ribosomal subunit protein eL30A / Large ribosomal subunit protein uL15B / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL32A / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL16A / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein uL23A / Large ribosomal subunit protein eL18B / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL43A / SDO1-like protein C21C3.19 / Large ribosomal subunit protein eL34B / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL15B / Large ribosomal subunit protein eL38A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL42

• Biological species: Schizosaccharomyces pombe 972h- (yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 2.8 Å
• Authors: Gluc M / Jomaa A

Funding support

United States, Germany, 2 items

Organization	Grant number	Country
National Science Foundation (NSF, United States)		United States
Boehringer Ingelheim Fonds (BIF)		Germany

• Citation: Journal: To Be Published; Title: A novel eukaryotic ribosome factor promotes translation restart after dormancy; Authors: Gluc M / Rosa H / Bozko M / Prince CR / Turner LA / Peskova Y / Feaga HA / Gould KL / Mattei S / Jomaa A

History

Deposition	Jul 9, 2025	-
Header (metadata) release	Apr 15, 2026	-
Map release	Apr 15, 2026	-
Update	Apr 15, 2026	-
Current status	Apr 15, 2026	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_71645.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: in vitro reconstituted complex of purified S. pombe large ribosomal subunit and SNOR
• Voxel size: X=Y=Z: 0.84 Å

Density

Contour Level	By AUTHOR: 0.002
Minimum - Maximum	-0.011136486 - 0.029578315
Average (Standard dev.)	0.000044635042 (±0.00095226493)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 430.08 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_71645_msk_1.map

Additional map: #1

• File: emd_71645_additional_1.map

Half map: half-map A

• File: emd_71645_half_map_1.map
• Annotation: half-map A

Half map: half-map B

• File: emd_71645_half_map_2.map
• Annotation: half-map B

Sample components

Entire : In vitro reconstituted complex of purified S. pombe large ribosom...

• Entire: Name: In vitro reconstituted complex of purified S. pombe large ribosomal subunit and SNOR

Components

• Complex: In vitro reconstituted complex of purified S. pombe large ribosomal subunit and SNOR
  • Protein or peptide: Large ribosomal subunit protein eL42
  • Protein or peptide: Large ribosomal subunit protein eL43A
  • RNA: 28S ribosomal RNA
  • RNA: 5S ribosomal RNA
  • RNA: 5.8S ribosomal RNA
  • Protein or peptide: Large ribosomal subunit protein uL2C
  • Protein or peptide: Large ribosomal subunit protein uL3A
  • Protein or peptide: Large ribosomal subunit protein uL4A
  • Protein or peptide: Large ribosomal subunit protein uL18B
  • Protein or peptide: Large ribosomal subunit protein eL6
  • Protein or peptide: Large ribosomal subunit protein uL30C
  • Protein or peptide: Large ribosomal subunit protein eL8
  • Protein or peptide: Large ribosomal subunit protein uL6B
  • Protein or peptide: Large ribosomal subunit protein uL16A
  • Protein or peptide: Large ribosomal subunit protein uL5A
  • Protein or peptide: Large ribosomal subunit protein eL13
  • Protein or peptide: Large ribosomal subunit protein eL14
  • Protein or peptide: Large ribosomal subunit protein eL15B
  • Protein or peptide: Large ribosomal subunit protein uL13A
  • Protein or peptide: Large ribosomal subunit protein uL22A
  • Protein or peptide: Large ribosomal subunit protein eL18B
  • Protein or peptide: Large ribosomal subunit protein eL19B
  • Protein or peptide: Large ribosomal subunit protein eL20A
  • Protein or peptide: Large ribosomal subunit protein eL21B
  • Protein or peptide: Large ribosomal subunit protein eL22
  • Protein or peptide: Large ribosomal subunit protein uL14B
  • Protein or peptide: Large ribosomal subunit protein eL24B
  • Protein or peptide: Large ribosomal subunit protein uL23A
  • Protein or peptide: Large ribosomal subunit protein uL24
  • Protein or peptide: Large ribosomal subunit protein eL27A
  • Protein or peptide: Large ribosomal subunit protein uL15B
  • Protein or peptide: Large ribosomal subunit protein eL29
  • Protein or peptide: Large ribosomal subunit protein eL30A
  • Protein or peptide: Large ribosomal subunit protein eL31
  • Protein or peptide: Large ribosomal subunit protein eL32A
  • Protein or peptide: Large ribosomal subunit protein eL33A
  • Protein or peptide: Large ribosomal subunit protein eL34B
  • Protein or peptide: Large ribosomal subunit protein uL29
  • Protein or peptide: Large ribosomal subunit protein eL36B
  • Protein or peptide: Large ribosomal subunit protein eL37B
  • Protein or peptide: Large ribosomal subunit protein eL38A
  • Protein or peptide: Large ribosomal subunit protein eL39
  • Protein or peptide: Large ribosomal subunit protein eL28
  • Protein or peptide: SDO1-like protein C21C3.19
• Ligand: ZINC ION

Supramolecule #1: In vitro reconstituted complex of purified S. pombe large ribosom...

• Supramolecule: Name: In vitro reconstituted complex of purified S. pombe large ribosomal subunit and SNOR; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)

Macromolecule #1: Large ribosomal subunit protein eL42

• Macromolecule: Name: Large ribosomal subunit protein eL42 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 12.239647 KDa

Sequence

String:

MVNIPKTRKT YCPGKNCRKH TVHRVTQYKK GPDSKLAQGK RRYDRKQSGF GGQTKPVFHK KAKVTKKVVL RLECVSCKYK NQLVLKRCK HFELGGEKKT KGAAIQF

UniProtKB: Large ribosomal subunit protein eL42

Macromolecule #2: Large ribosomal subunit protein eL43A

• Macromolecule: Name: Large ribosomal subunit protein eL43A / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 10.444286 KDa

Sequence

String:

MTKRTKKVGV TGKYGVRYGA SLRRDVRKIE VQQHSRYQCP FCGRNTVKRT AAGIWCCNGK GCKKVLAGGA WTVTTAAATS ARSTIRRLR EMVEV

UniProtKB: Large ribosomal subunit protein eL43A

Macromolecule #6: Large ribosomal subunit protein uL2C

• Macromolecule: Name: Large ribosomal subunit protein uL2C / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 27.151246 KDa

Sequence

String:

MGRVIRAQRK SGGIFQAHTR LRKGAAQLRT LDFAERHGYI RGVVQKIIHD PGRGAPLAKV AFRNPYHYRT DVETFVATEG MYTGQFVYC GKNAALTVGN VLPVGEMPEG TIISNVEEKA GDRGALGRSS GNYVIIVGHD VDTGKTRVKL PSGAKKVVPS S ARGVVGIV AGGGRIDKPL LKAGRAFHKY RVKRNCWPRT RGVAMNPVDH PHGGGNHQHV GHSTTVPRQS APGQKVGLIA AR RTGLLRG AAAVEN

UniProtKB: Large ribosomal subunit protein uL2C

Macromolecule #7: Large ribosomal subunit protein uL3A

• Macromolecule: Name: Large ribosomal subunit protein uL3A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 43.889832 KDa

Sequence

String:

MSHCKFEQPR HGSLGFLPRK RASRQRGKVK AFPKDDASKP VHLTAFLGYK AGMTHIVRDL DRPGSKMHKR EILEAVTVIE TPPMVVVGV VGYVETPRGL RSLTTVWAEH LSEEVKRRFY KNWFKSKKKA FTKYAKKYAE STQSINRELE RIKKYCSVVR V LAHTQIRK TPLAQKKAHL MEIQVNGGSV ADKVEWAREH FEKTVDIKST FEQNEMIDVI GVTRGKGNEG TTARWGTKRL PR KTHRGLR KVACIGAWHP ANVQWTVARA GNAGYMHRTQ LNSKIYRIGA GDDAKNASTD FDATEKRITP MGGFVRYGVV END FVMLNG ATPGPVKRVL TLRKSLLTHT SRKALEPVSL KWIDTASKFG HGRFQTPAEA KQFLGTLKKD VA

UniProtKB: Large ribosomal subunit protein uL3A

Macromolecule #8: Large ribosomal subunit protein uL4A

• Macromolecule: Name: Large ribosomal subunit protein uL4A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 39.839129 KDa

Sequence

String:

MAAARPTVSI YSKDGSVSSE TIALPFVFKA PIRPDLVRSV HTAVAKNKRQ PYAVSEKAGH QTSAESWGTG RALARIPRVG GGGTHRSGQ AAFGNMCRSG RMFAPTKTWR KWHVKVNQNE KRYAISSAVA ASGVPSLLLA RGHRIEEIPE VPLVVDDAVQ S FQKTKEAV ALLKEIKAYR DVVKVANSRK LRAGKGKLRN RRHVQRRGPL VVFNEDAGIV KAFRNIPGVE IVNVRRLNLL QL APGGHLG RFVIWTKSAF GLLDSVFGST TEAAQLKKNY FLPENIISNA DVTRLINSDE IQSIVKAAGP SRVKRAHVQK KNP LKNKAV LARLNPYAKA YKANVKLNTG KTPKAAGEKF LTVLHEN

UniProtKB: Large ribosomal subunit protein uL4A

Macromolecule #9: Large ribosomal subunit protein uL18B

• Macromolecule: Name: Large ribosomal subunit protein uL18B / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 33.538898 KDa

Sequence

String:

MPFIKAVKSS PYFSRYQTKY RRRREGKTDY YARKRLIAQA KNKYNAPKYR LVVRFSNRFV TCQIVSSRVN GDYVLAHAHS SELPRYGIK WGLANWTAAY ATGLLVARRA LAKVGLADKY EGVTEPEGEF ELTEAIEDGP RPFKVFLDVG LKRTSTGSRV F GAMKGASD GGLFIPHSPN RFPGFDIETE ELDDETLRKY IYGGHVAEYM EMLIDDDEER YQKQFSGLIA DGIESDQLED IY AEAYAKI REDPSFQKSG KDAAAFKAES LKYTQRKLTA EERKERFNAK VIEAGRA

UniProtKB: Large ribosomal subunit protein uL18B

Macromolecule #10: Large ribosomal subunit protein eL6

• Macromolecule: Name: Large ribosomal subunit protein eL6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 21.285996 KDa

Sequence

String:

MSTVKVNGAK NGGERMVLPA GEAAAKYYPA YRENVPKKAR KAVRPTKLRA SLAPGTVCIL LAGRFRGKRV VVLSQLEDTL VVTGPYKVN GVPIRRVNHR YVIATSAPKI DVSGVSVEKF TKAYFAKQKR SGPVKKDEAF FAENAPKNAL PAERIADQKA V DAKLLPAI KAIPNMKEYL AASFALSNGD RPHLMKF

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #11: Large ribosomal subunit protein uL30C

• Macromolecule: Name: Large ribosomal subunit protein uL30C / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 28.780457 KDa

Sequence

String:

MAVASSTVPS KEQIFAPESL LKKKKTQEQS REQRVAAAAE KKAAQQKKRE LIAKRAESYD AEYRKAEREQ IELGRKARAE GNYYVPDET KLVFVIRIRG INNIPPKARK IMQLLRLIQI NNGVFVKFNK ATKEMLQVVE PYVTYGIPNL KTVRELLYKR G FGKVNKQR IALSDNAIIE AALGKYSILS IEDLIHEIYT VGPNFKQAAN FIWPFQLSSP LGGWRDRKFK HFIEGGDAGK RD EHINSLV RKML

UniProtKB: Large ribosomal subunit protein uL30C

Macromolecule #12: Large ribosomal subunit protein eL8

• Macromolecule: Name: Large ribosomal subunit protein eL8 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 28.659777 KDa

Sequence

String:

MAPKSKKVAP SPFAQPKAAK TTKNPLFVSR PRSFGIGQDI QPKRDLSRFV KWPEYIRLQR RRKILNLRLK VPPAIAQFQK TLDKNTATQ VFKLLNKYRP ETAAEKKQRL VAEAEAVANG KSAQDVSKKP YNVKYGLNHV VALIEAKKAK LVLIASDVDP I ELVVFLPA LCKKMGVPYA IVKNKARLGT VIHQKTAAVL AVTEVREEDK NELASIVSAV DANFSAKYDE SRRKWGGGIM GG KTQALLA KRAKAAAATV RL

UniProtKB: Large ribosomal subunit protein eL8

Macromolecule #13: Large ribosomal subunit protein uL6B

• Macromolecule: Name: Large ribosomal subunit protein uL6B / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 21.413887 KDa

Sequence

String:

MGRDIYKDET LTIPEGVSVD IKARLVTVKG PRGVLKQNLR RVDIELKKQG NTIKFIVWHG SRKHNACIRT AYSIINNMII GVTQGFRYK MRLVYAHFPI NINLTENGTV VEIRNFLGER ITRVIKCLPG VTVSISSAVK DEIIIEGNSL ENVSQSAANI K QICNVRNK DIRKFLDGIY VSERGNIEEL E

UniProtKB: Large ribosomal subunit protein uL6B

Macromolecule #14: Large ribosomal subunit protein uL16A

• Macromolecule: Name: Large ribosomal subunit protein uL16A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 25.421596 KDa

Sequence

String:

MARRPARCYR YCKNKPYPKS RYNRAVPDSK IRIFDLGRKR AGVDEFPLCI HLVSNEYEQI TSEALEAARI CANKYLVKIG GKDSFHLRV RAHPFHVVRI NKMLSCAGAD RLQTGMRHAF GKPNGLVARV NIGQILMSVR TKDSSRATAI EALRRCQYKF P GQQRIIVS KKWGFSQYAR DEYIEKRSRG EIIPDGCYAK FLNKRGSLQE KLDLFPEASF NLA

UniProtKB: Large ribosomal subunit protein uL16A

Macromolecule #15: Large ribosomal subunit protein uL5A

• Macromolecule: Name: Large ribosomal subunit protein uL5A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 19.928182 KDa

Sequence

String:

MAEKAQNPMK ELRISKLVLN ISLGESGDRL TRAAKVLEQL SGQTPVFSKA RYTIRRFGIR RNEKIACHVT VRGPKAEEIL ERGLKVKEY ELKKRNFSAT GNFGFGIQEH IDLGIKYDPS IGIYGMDFYV VMDRPGMRVA RRKAQRGRVG YTHKINAEDT I NWFKQKYD AVVLGK

UniProtKB: Large ribosomal subunit protein uL5A

Macromolecule #16: Large ribosomal subunit protein eL13

• Macromolecule: Name: Large ribosomal subunit protein eL13 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 23.578145 KDa

Sequence

String:

MAIHVKGQLP NAHFHKDWQR YVKTWFNQPG RKLRRRQARQ TKAAKIAPRP VEAIRPAVKP PTIRYNMKVR AGRGFTLEEL KAAGVSRRV ASTIGIPVDH RRRNRSEESL QRNVERIKVY LAHLIVFPRK AGQPKKGDAT DVSGAEQTDV AAVLPITQEA V EEAKPITE EAKNFNAFST LSNERAYARY AGARAAFQKK RAEEAEAKKK

UniProtKB: Large ribosomal subunit protein eL13

Macromolecule #17: Large ribosomal subunit protein eL14

• Macromolecule: Name: Large ribosomal subunit protein eL14 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 15.241078 KDa

Sequence

String:

MEGFKRYVEV GRVVLVTKGE YTGKLAVIVD IVDHKRALID SPCSEFPRQV IRYGSVVLTH IVMKLPRGAR SGIVAKKWKA QDVCNKWAS SAWAKKLEAK KVRSQLNDFD RFAVMRLKKQ RREQVNVAVA KALKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #18: Large ribosomal subunit protein eL15B

• Macromolecule: Name: Large ribosomal subunit protein eL15B / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 23.878609 KDa

Sequence

String:

MGAYKYLEEL AKKKQSDVNL FLSRVRAWEY RQMNVIHRAS RPSRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGQT YGKPVHQGV NHLKYQRSAR CTAEERVGRY CSNLRVLNSY WVNQDATYKF FEVILVDPSH KAIRRDPRIN WIVNPVHKHR E SRGLTSIG KKSRGIGKGH RYNNSPQHAT WLRHNTLSLR RYR

UniProtKB: Large ribosomal subunit protein eL15B

Macromolecule #19: Large ribosomal subunit protein uL13A

• Macromolecule: Name: Large ribosomal subunit protein uL13A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 22.050088 KDa

Sequence

String:

MSEFQKVVVI DAKGHLLGRL ASVVAKQLLG GQKVVVVRCE ELNISGHFFR NKLKYLAYLR KACRYNPSRG AFHFRAPSRI FQKAVRGML PHKTARGQAA LEHLQAVEGI PPPFDKQKRV VVPAALRVLR LKPGRKYCTV GRLSSEVGWK YSDIVSKLEE R RKVKSAAF YQAKLAKQKK IASAKAASSV NGKLAEFGY

UniProtKB: Large ribosomal subunit protein uL13A

Macromolecule #20: Large ribosomal subunit protein uL22A

• Macromolecule: Name: Large ribosomal subunit protein uL22A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 20.854113 KDa

Sequence

String:

MVRYSASPAL ETKCAKARGA YLRTHFKNSR EVAFTINGMS LKKAFIFLDN VKEHKQAVPF RRFNGGVGRT AQGKEFGVTQ ARWPVKSVK FFYDLLKNAE ANAEAKGLDM DKLIIKHVQV NAAPKQRRRT YRAHGRVTAY LSSPSHIEII VAEEEEAVPK A NDTVSRVS LKQGAKARNL AARKAITAA

UniProtKB: Large ribosomal subunit protein uL22A

Macromolecule #21: Large ribosomal subunit protein eL18B

• Macromolecule: Name: Large ribosomal subunit protein eL18B / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 21.234957 KDa

Sequence

String:

MGIDIERHHV RKSQRSKPAS ENVYLKLLVK LYRFLARRTD SRFNKAILKR LFQSKTNRPP ISISKIAALT SRKSASLEGK TTVIVGTVT DDERLLTVPK LSVAALRFTK SARARILKAG GEVLTLDQLA LRAPTGSNTV LLRGKKHARE AYRHFGFGPH K HKAPYVRS EGRKFERARG RRKSRAFKV

UniProtKB: Large ribosomal subunit protein eL18B

Macromolecule #22: Large ribosomal subunit protein eL19B

• Macromolecule: Name: Large ribosomal subunit protein eL19B / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 22.813801 KDa

Sequence

String:

MANLRTQKRL AASVLKCGKR KVWMDPNEIS EISNANSRQN IRKLVKDGLV IRKPNLMHSR FRIRKTHAAK RLGRHTGYGK RKGTAEARM PSTVVWMRRQ RVLRRLLRKY RESGKIDKHL YHTLYLEAKG NTFKHKRALI EHIQRAKAEA NRTKLIQEQQ D ARRARAKA ARQRRAKAVE EKREQIYTAA EKIEE

UniProtKB: Large ribosomal subunit protein eL19B

Macromolecule #23: Large ribosomal subunit protein eL20A

• Macromolecule: Name: Large ribosomal subunit protein eL20A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 20.642348 KDa

Sequence

String:

MALKEYQVVG RKVPTEHEPV PKLFRMRLFA PNESVAKSRY WYFLKMINKV KKATGEIVAI NEISEPKPLK AKVFGIWIRY DSRSGTHNM YKEFRDTTRV GAVEAMYADM AARHRARFRS IRILKVVEVE KKEDVRRNYV KQLLNPHLKF PLPHRRTGVV G LAGKKVFA PHRPSTFY

UniProtKB: Large ribosomal subunit protein eL20A

Macromolecule #24: Large ribosomal subunit protein eL21B

• Macromolecule: Name: Large ribosomal subunit protein eL21B / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 18.39017 KDa

Sequence

String:

MPHSYGIRAR TRYTFQRGFR EHGQIRLSTY LKTYKVGDIV DIKVNGAVQK GMPHKYYHGK TGVVYNVTQS SVGVLIYKVV GNRYMEKRV NVRIEHVKHS KCRQDFLDRV KANEAKRKEA KAQGKTVQLR RQPAPPATAH FVSTENNEPV TLHPVAYDTT I

UniProtKB: Large ribosomal subunit protein eL21B

Macromolecule #25: Large ribosomal subunit protein eL22

• Macromolecule: Name: Large ribosomal subunit protein eL22 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 13.344412 KDa

Sequence

String:

MVKKNTKVSN KYIIDATAAV NDKIFDVAAF EKYLIDRIKV DGKTGNLGSS VVVSREGSSK IAVIAHIDFS GRYLKYLTKK FLKKHSLRD WLRVVSTKKG VYELRYYNVV VGNDEEEQ

UniProtKB: Large ribosomal subunit protein eL22

Macromolecule #26: Large ribosomal subunit protein uL14B

• Macromolecule: Name: Large ribosomal subunit protein uL14B / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 14.905514 KDa

Sequence

String:

MSRGRGAASG TKYRMTLGLP VQAIMNCADN SGAKNLYIVS VFGTGARLNR LPAASCGDMV LATVKKGKPD LRKKIMPAIV VRQRKAWRR KDGVYLYFED NAGVIVNPKG EMKGSAITGP VAKECADLWP RIASNAGTVV

UniProtKB: Large ribosomal subunit protein uL14B

Macromolecule #27: Large ribosomal subunit protein eL24B

• Macromolecule: Name: Large ribosomal subunit protein eL24B / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 16.957908 KDa

Sequence

String:

MKVEVCSFSG SKVYPGAGRL FVRGDNKVFR FVNKKSESLF LQRKNPRRLS WTVLYRRMHK KGISEEHAKK RTRRTVKHQR GIVGANLDV IKEKRNQRPE VRAAARAAAL KQRKDKRAAS ESEKKAIKAK SAASSARGQA IKNAKVAARR

UniProtKB: Large ribosomal subunit protein eL24B

Macromolecule #28: Large ribosomal subunit protein uL23A

• Macromolecule: Name: Large ribosomal subunit protein uL23A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 15.870718 KDa

Sequence

String:

MSVAKAKGAQ KTVQKGIHNK VAKKVRTSTT FRRPKTLQLS RKPKYARKSV AHAPRLDEYK IIVNPINSES AMKKIEDDNT LVFHVHLKA NKFTIKEAVR KLYSVEPVKI NTLIRPNGTK KAFVKLSADA DALDVANRIG FL

UniProtKB: Large ribosomal subunit protein uL23A

Macromolecule #29: Large ribosomal subunit protein uL24

• Macromolecule: Name: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 14.375912 KDa

Sequence

String:

MKFSRDVTSS RRKQRKAHFG APSSVRRVLM SAPLSKELRE QYKIRSLPVR RDDQITVIRG SNKGREGKIT SVYRKKFLLL IERVTREKA NGASAPVGID ASKVVITKLH LDKDRKDLIV RKGGKVE

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #30: Large ribosomal subunit protein eL27A

• Macromolecule: Name: Large ribosomal subunit protein eL27A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 15.421304 KDa

Sequence

String:

MVKILKPGKV ALITRGRFAG KKVVILQAID QGSKSHPFGH AVVAGVERYP LKVTKSMGAK RIARRSRVKP FIKVVNYNHL MPTRYALEL DNLKGLITAD TFKEPTQRSA ARKTVKKTFE EKYQSGKSAW FFTPLRF

UniProtKB: Large ribosomal subunit protein eL27A

Macromolecule #31: Large ribosomal subunit protein uL15B

• Macromolecule: Name: Large ribosomal subunit protein uL15B / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 16.723518 KDa

Sequence

String:

MPTHTSKTRK LRGHVSAGHG RIGKHRKHPG GRGKAGGLQH LRSHFDKYHP GYFGKVGMRR FHLMKNPLWR PTVNLDRLWT LVPNETREK YLGKNTEVAP VINVLQSGYG KVLGKGRLPD TPVIIQTRYV SRRAEEKIKQ AGGVVELIA

UniProtKB: Large ribosomal subunit protein uL15B

Macromolecule #32: Large ribosomal subunit protein eL29

• Macromolecule: Name: Large ribosomal subunit protein eL29 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 7.224249 KDa

Sequence

String:

MAKSKNHTNH NQNKKAHRNG IKRPQKHRYD SLKYRDAKFR RNQKFANRGT VEAIRQAKAS A

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #33: Large ribosomal subunit protein eL30A

• Macromolecule: Name: Large ribosomal subunit protein eL30A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 11.610639 KDa

Sequence

String:

MASVVTKKSK KSGDTINAKL ALTMKSGKYV LGYKSTLKTL RSGKAKLILI AGNCPPLRKS ELEYYAMLSK ANVHHYAGTN IDLGTACGK LFRVGVLAIT DAGDSDILDA

UniProtKB: Large ribosomal subunit protein eL30A

Macromolecule #34: Large ribosomal subunit protein eL31

• Macromolecule: Name: Large ribosomal subunit protein eL31 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 13.290468 KDa

Sequence

String:

MANTKKSAIN QVVTRDYTIH MHKRLYGVSF KKRAPRAIKE IVAFAQKHMQ TKEVRVDPSL NKEVWKRGIR NVPHRLRLRL SRKRSDEDD KALYTYVQAV DVANPKMETT VVEE

UniProtKB: Large ribosomal subunit protein eL31

Macromolecule #35: Large ribosomal subunit protein eL32A

• Macromolecule: Name: Large ribosomal subunit protein eL32A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 14.491099 KDa

Sequence

String:

MAAVNIIKKR TKPFKRHQSD RFKRVGESWR KPRGIDSCVR RRFRGTISMP KIGYGNNKKT RYCMPNGLKA FLVRNVSDVE LLLMHNKTY AAEIAGNVSA RKRVEIVEKA RALGVKVTNA GAKVRSQE

UniProtKB: Large ribosomal subunit protein eL32A

Macromolecule #36: Large ribosomal subunit protein eL33A

• Macromolecule: Name: Large ribosomal subunit protein eL33A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 12.212304 KDa

Sequence

String:

MPAQGHRLYV KAKHLSFQRS KHVIHPGTSL VKIEGCDSKE EAQFYLGKRI CFVYKSNKPV RGSKIRVIWG TVSRPHGNSG VVRARFTHN LPPKTFGASL RVMLYPSNV

UniProtKB: Large ribosomal subunit protein eL33A

Macromolecule #37: Large ribosomal subunit protein eL34B

• Macromolecule: Name: Large ribosomal subunit protein eL34B / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 12.828181 KDa

Sequence

String:

MAQRVTYRRR LAYNTRSNKT RIIKTPGNNI RYLHIKKLGT IPRCGDTGVP LQGIPALRPR EFARLSHNQK KVQRAYGGCL SANAVKDRI VRAFLIEEQK IVKQKLKQMS QK

UniProtKB: Large ribosomal subunit protein eL34B

Macromolecule #38: Large ribosomal subunit protein uL29

• Macromolecule: Name: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 14.333901 KDa

Sequence

String:

MALKTFELRK QSQENLAEQL QELRQELASL RVQKIAGGSG SKLSKIKTTR KDIARILTVI NESNRLAARE AYKNKKYIPL DLRQKKTRA IRRALTPYEQ SRKTLKQIKK ERYFPLRKYA LKA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #39: Large ribosomal subunit protein eL36B

• Macromolecule: Name: Large ribosomal subunit protein eL36B / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 11.287438 KDa

Sequence

String:

MAPGLVVGLN KGKTLTKRQL PERPSRRKGH LSKRTAFVRS IVREVAGFAP YERRVMELIR NSQDKRARKL AKKRLGTLKR AKGKIEELT SVIQSSRLAH

UniProtKB: Large ribosomal subunit protein eL36B

Macromolecule #40: Large ribosomal subunit protein eL37B

• Macromolecule: Name: Large ribosomal subunit protein eL37B / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 10.112824 KDa

Sequence

String:

MTKGTQSFGM RHNKSHTICR RCGKRSFHIQ KSTCACCGYP AAKTRSYNWG AKAKRRRTTG TGRMSYLKKV HRSFKNGFRS GKPAAAVAA SA

UniProtKB: Large ribosomal subunit protein eL37B

Macromolecule #41: Large ribosomal subunit protein eL38A

• Macromolecule: Name: Large ribosomal subunit protein eL38A / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 8.511081 KDa

Sequence

String:

MPRQISDIKQ FLEIARRKDA TSARIKKNTN KDVKFKLRCS KYLYTLVVAD AKKAEKLRQS LPPDLTVTEV GKKA

UniProtKB: Large ribosomal subunit protein eL38A

Macromolecule #42: Large ribosomal subunit protein eL39

• Macromolecule: Name: Large ribosomal subunit protein eL39 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 6.326514 KDa

Sequence

String:

MPSHKSFRTK QKLAKAARQN RPIPQWIRLR TGNTVHYNMK RRHWRRTKLN I

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #43: Large ribosomal subunit protein eL28

• Macromolecule: Name: Large ribosomal subunit protein eL28 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 14.781037 KDa

Sequence

String:

MSVSNDLIWQ VIRDNNRFLV KRPEFGGIQF NREPVNVSGK NAQRFSGLCN DKAVGVQANS PRGVVLITKT NPKNAQKPAK LFRKDVIAN ASSRKTYKSI AGRIGRTGYR DDLVKVSVAR ASAILSSQRP KKTVA

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #44: SDO1-like protein C21C3.19

• Macromolecule: Name: SDO1-like protein C21C3.19 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 11.909361 KDa
• Recombinant expression: Organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)

Sequence

String:

MSSSKANQTR VCYQPEDTTF IIIASNGPDV MRWRKDKTVP LTEIVDSFQV FTTSNNKGNE GQLITASKQE LENTFGTSKD VDVVTKILT DGKIIPHREH GKHKEVN

UniProtKB: SDO1-like protein C21C3.19

Macromolecule #3: 28S ribosomal RNA

• Macromolecule: Name: 28S ribosomal RNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 1.1298282499999999 MDa

Sequence

String:

AUUUGACCUC AAAUCAGGUA GGACUACGCG CUGAACUUAA GCAUAUCAAU AAGCGCAGGA AAAGAAAAUA ACCAUGAUUC CCUCAGUAA CGGCGAGUGA AGCGGGAAAA GCUCAAAUUU GAAAUCUGGC AACAUUUCUU UUGUUGUCCG AGUUGUAAUU U CAAGAAGC UGCUUUGAGU GUAGACGAUC GGUCUAAGUU CCUUGGAACA GGACGUCAGA GAGGGUGAGA ACCCCGUCUU UG GUCGAUU GGAUAUGCCA UAUAAAGCGC UUUCGAAGAG UCGAGUUGUU UGGGAAUGCA GCUCUAAAUG GGUGGUAAAU UUC AUCUAA AGCUAAAUAU UGGCGAGAGA CCGAUAGCGA ACAAGUAGAG UGAUCGAAAG AUGAAAAGAA CUUUGAAAAG AGAG UUAAA UAGUACGUGA AAUUGCUGAA AGGGAAGCAU UGGAAAUCAG UCUUACCUGG GUGAGAUCAG UAGUCUCUUC GCGAG ACUA UGCACUCUGA ACCUGUGGUA GGUCAGCAUC AGUUUUCGGG GGCGGAAAAA GAAUAAGGGA AGGUGGCUUU CCGGGU UCU GCCUGGGGAG UGUUUAUAGC CCUUGUUGUA AUACGUCCAC UGGGGACUGA GGACUGCGGC UUCGUGCCAA GGAUGCU GA CAUAAUGGUU UUCAAUGGCC CGUCUUGAAA CACGGACCAA GGAGUCUAGC AUCUAUGCGA GUGUUUGGGU GAUGAAAA C CCAUCCGCGA AAUGAAAGUG AAUGCAGGUG GGAACGCCCU UGUGGCGUGC ACCAUCGACC GACCCGGAAG UUUGUCAAU GGAAGGGUUU GAGUAAGAGC AUAGCUGUUG GGACCCGAAA GAUGGUGAAC UAUGCCUGAA UAGGGUGAAG CCAGAGGAAA CUCUGGUGG AGGCUCGUAG AGAUUCUGAC GUGCAAAUCG AUCUUCAAAU UUGGGUAUAG GGGCGAAAGA CUAAUCGAAC C AUCUAGUA GCUGGUUCCU GCCGAAGUUU CCCUCAGGAU AGCAGAAACU CAGAUCAGUU UUAUGAGGUA AAGCGAAUGA UU AGAGGUC UUGGGGAAGG AAUUUCCUCA ACCUAUUCUC AAACUUUAAA UAUGUAAGAC GCCCUUGUCG CUUAAUUGGA CGU GGGCCA UCGAAUGAGA GUUUCUAGUG GGCCAUUUUU GGUAAGCAGA ACUGGCGAUG CGGGAUGAAC CGAACGUGAG GUUA AGGUG CCGGAAUGUA CGCUCAUCAG ACACCAGAAA AGGUGUUAGU UCAUCUAGAC AGCAGGACGG UGGCCAUGGA AGUCG GAAU CCGCUAAGGA GUGUGUAACA ACUCACCUGC CGAAUGAACU AGCCCUGAAA AUGGAUGGCG CUUAAGCGUA CUACCC AUA CCUCACCGUC UGGGUUAGCU UUGAGAAGCU CAGACGAGUA GGCAGGCGUG GAGGUUUGUG ACGAAGCCUU GGGCGUG AG CCUGGGUCGA ACAGCCUCUA GUGCAGAUCU UGGUGGAAGU AGCAAAUAUU CAAAUGAGAA CUUUGAAGAC UGAAGUGG G GAAAGGUUCC AUGUGAACAG CAGUUGGACA UGGGUUAGUC GAUCCUAAGA GAUAGGGAAG CUCCGUAUGA AAGUUGCAC GAUUUUUCGU GCCUCCUAUC GAAAGGGAAU CCGGUUAAUA UUCCGGAACC AGAAGGUGGA AUCAACACGG CAACGUAAAU GAAGUUGGA GACGUCGGCG GGAGCCCUGG GAAGAGUUCU CUUUUCUUUU UAACAAACCA UUGAACUACC CUGAAAUCGG U UUAUCCGG AGCUAGGGUA UGGUGUUUGG AAGAGUUCAG CGCCUCAUGC UGAAUCCGGU GCGCUCUCGA CGGCCCUUGA AA AUCCAAC GGAAGAAUGG ACCUUCGGGU CCUUGUUUUC ACAUCUGGUC GUACUCAUAA CCGCAGCAGG UCUCCAAGGU GAA CAGCCU CUAGUUGAUA GAACAAUGUA GAUAAGGGAA GUCGGCAAAA UGGAUCCGUA ACUUCGGGAU AAGGAUUGGC UCUA AGGGU UGGGUACGUU GGGCCUUGGA ACCUGAACGG UUGCUGGACU GAGCGUGGAC CGAUGUCUUU UCUCGCCUUU CGGGG UGAG AAGGGAUGUU GGACCUGCUU GGACCUUGGC GGCCGGGAAG UCCUUGGUCG GGCUUUUCUC CUUCUCGGGG AUUAUG CUC UUACUGGCGU ACGUUUAACA ACCAACUUAG AACUGGUACG GACAAGGGGA AUCUGACUGU CUAAUUAAAA CAUAGCA UU GCGAUGGCCA GAAAGUGGUG UUGACGCAAU GUGAUUUCUG CCCAGUGCUC UGAAUGUCAA AGUGAAGAAA UUCAACCA A GCGCGGGUAA ACGGCGGGAG UAACUAUGAC UCUCUUAAGG UAGCCAAAUG CCUCGUCAUC UAACUAGUGA CGCGCAUGA AUGGAUUAAC GAGAUUCCCA CUGUCCCUAU CUACUAUCUA GCGAAACCAC AGCCUGGGGA ACGGGCCAGG CAAAAUCAGC GGGGAAAGA AGACCCUGUU GAGCUUGACU CUAGUUUGAC AUUGUGAAGA GACAUAGAGG GUGUAGGAUA AGUGGGAGUA U GUUUCGGC AUACGCCGGU GAAAUACCAC UACCUUUAUC GUUUCUUUAC UUAAUCAAUG AAGCGGAAUU GGGAUUUAUU UC CCAUAUU CUAGCGUUAA AGUUUCUUCG CGAACUGAUC CGCGUUGAUG ACAUUGUCAG GUGGGGAGUU UGGCUGGGGC GGC ACAUCU GUUAAAAGAU AACGCAGGUG UCCUAAGGGG GACUCAUCGA GAACAGAAAU CUCGAGUAGA AUAAAAGGGU AAAA GUCCC CUUGAUUUUG AUUUUCAGUG UGAAUACAAA CCAUGAAAGU GUGGCCUAUC GAUCCUUUGU UCCCUCGAAA UUUGA GGAC AGAGGUGCCA GAAAAGUUAC CACAGGGAUA ACUGGCUUGU GGCAGCCAAG CGUUCAUAGC GACGUUGCUU UUUGAU UCU UCGAUGUCGG CUCUUCCUAU CAUACCGAAG CAGAAUUCGG UAAGCGUUGG AUUGUUCACC CACUAAUAGG GAACGUG AG CUGGGUUUAG ACCGUCGUGA GACAGGUUAG UUUUACCCUA CUGAUGAAGU GUCGUCGCAA UGGUAAUUCA ACUUAGUA C GAGAGGAACC GUUGAUUCAG AUCAUUGGUA UUUGCGGCUG CCUGACAAGG CAAUGCCGCG GAGCUAUCAU CUGCCGGAU AACGGCUGAA CGCCUCUAAG CCAGAAUCCG UGCCAGAAAG CGACGAUUUU UUGGUCCGCA UGAUUUAUAU GUAUAAAAAU AGAGGUAGG ACUUGUUCCU ACUCUCCUGU AUCGUAGAAG AUGGGCGAUG GUUGAUGAAA CGGAAGUGUU UUAUUGACUU G UCCAUGAA AUUCCAUUGA AAUCUUGUGC GGAAUCGAAU CCAUUGCAUA CGACUUUAAU GUGGAACGGG GUAUUGUAAG CA GUAGAGU AGCCUUGUUG UUACGAUCUG CUGAGAUUAA GCCUUUGUUC CCAAGAUUUG U

GENBANK: GENBANK: CU329672.1

Macromolecule #4: 5S ribosomal RNA

• Macromolecule: Name: 5S ribosomal RNA / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 78.518867 KDa

Sequence

String:

UUAAACGUCU ACGGCCAUAC CUAGGCGAAA ACACCAGUUC CCGUCCGAUC ACUGCAGUUA AGCGUCUGAG GGCCUCGUUA GUACUAUGG UUGGAGACAA CAUGGGAAUC CGGGGUGCUG UAGGCUUCCU UUUAUUCUUU UUGCUUUUUG UUUGAGCUCA U UUGGGAAU AAUAAUCAUU UACCUUGAUU CGUACUUAUU CAUUUCAAUA CCUUUGCACA UAAUAUUAUU GUGUACUAUG AA UGAUAU

GENBANK: GENBANK: CU329671.1

Macromolecule #5: 5.8S ribosomal RNA

• Macromolecule: Name: 5.8S ribosomal RNA / type: rna / ID: 5 / Number of copies: 1
• Source (natural): Organism: Schizosaccharomyces pombe 972h- (yeast)
• Molecular weight: Theoretical: 52.880188 KDa

Sequence

String:

AUUAUUUAAA ACUUUCAGCA ACGGAUCUCU UGGCUCUCGC AUCGAUGAAG AACGCAGCGA AAUGCGAUAC GUAAUGUGAA UUGCAGAAU UCCGUGAAUC AUCGAAUCUU UGAACGCACA UUGCGCCUUU GGGUUCUACC AAAGGCAUGC CUGUUUGAGU G UCAUU

GENBANK: GENBANK: Z19578.1

Macromolecule #45: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 45 / Number of copies: 3 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.25 mg/mL
• Buffer: pH: 7.4
• Grid: Model: Quantifoil R2/1 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: DARK FIELD / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.6 µm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: OTHER
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3) / Number images used: 110548
• Initial angle assignment: Type: PROJECTION MATCHING
• Final angle assignment: Type: PROJECTION MATCHING

Atomic model buiding 1

• Refinement: Space: REAL / Protocol: AB INITIO MODEL

Output model

PDB-9phc:
In vitro reconstituted complex of purified S. pombe large ribosomal subunit and SNOR