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- EMDB-71472: Negative stain EM map 3 of polyclonal serum from mouse immunized ... -

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Basic information

Entry
Database: EMDB / ID: EMD-71472
TitleNegative stain EM map 3 of polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored in complex with TX24-I53_dn5B.
Map dataNegative stain EM map of polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored in complex with TX24-I53_dn5B.
Sample
  • Complex: Polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored in complex with TX24-I53_dn5B.
    • Complex: Polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored
    • Complex: A/Texas/37/2024 HA-I53_dn5B
KeywordsFab / Serum / Influenza Hemagglutinin / Immune Complex / IMMUNE SYSTEM
Biological speciesMus musculus (house mouse) / Influenza A virus
Methodsingle particle reconstruction / negative staining / Resolution: 15.82 Å
AuthorsDosey A / King NP
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)1U19AI181881-01 United States
CitationJournal: To Be Published
Title: Stabilization of H5 highly pathogenic avian influenza hemagglutinin improves neutralizing antibody responses
Authors: Dosey A / King NP
History
DepositionJun 26, 2025-
Header (metadata) releaseFeb 4, 2026-
Map releaseFeb 4, 2026-
UpdateFeb 4, 2026-
Current statusFeb 4, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_71472.png

Downloads & links

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Map

FileDownload / File: emd_71472.map.gz / Format: CCP4 / Size: 27 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationNegative stain EM map of polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored in complex with TX24-I53_dn5B.
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.49 Å/pix.
x 192 pix.
= 478.08 Å		2.49 Å/pix.
x 192 pix.
= 478.08 Å		2.49 Å/pix.
x 192 pix.
= 478.08 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.49 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.91
Minimum - Maximum-0.9189817 - 4.549309
Average (Standard dev.)-0.017224805 (±0.1600023)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions192192192
Spacing192192192
CellA=B=C: 478.08002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Negative stain EM map of polyclonal serum from...

Fileemd_71472_half_map_1.map
AnnotationNegative stain EM map of polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored in complex with TX24-I53_dn5B.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Negative stain EM map of polyclonal serum from...

Fileemd_71472_half_map_2.map
AnnotationNegative stain EM map of polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored in complex with TX24-I53_dn5B.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane...

EntireName: Polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored in complex with TX24-I53_dn5B.
Components
  • Complex: Polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored in complex with TX24-I53_dn5B.
    • Complex: Polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored
    • Complex: A/Texas/37/2024 HA-I53_dn5B

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Supramolecule #1: Polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane...

SupramoleculeName: Polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored in complex with TX24-I53_dn5B.
type: complex / ID: 1 / Parent: 0

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Supramolecule #2: Polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane...

SupramoleculeName: Polyclonal serum from mouse immunized with H5 TX24-FMLMI-membrane anchored
type: complex / ID: 2 / Parent: 1
Source (natural)Organism: Mus musculus (house mouse)

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Supramolecule #3: A/Texas/37/2024 HA-I53_dn5B

SupramoleculeName: A/Texas/37/2024 HA-I53_dn5B / type: complex / ID: 3 / Parent: 1
Source (natural)Organism: Influenza A virus / Strain: A/Texas/37/2024

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Experimental details

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Structure determination

Methodnegative staining
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
StainingType: NEGATIVE / Material: Uranyl Acetate

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Electron microscopy

MicroscopeTFS TALOS L120C
Image recordingFilm or detector model: FEI CETA (4k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 120 kV / Electron source: LAB6
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Talos L120C / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE / Details: Ab initio reconstruction
Final reconstructionResolution.type: BY AUTHOR / Resolution: 15.82 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 13781
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: cryoSPARC
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: cryoSPARC

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