|Entry||Database: EMDB / ID: 7123|
|Title||Cryo-EM structure of rat TRPV6* in nanodiscs|
|Map data||primary map|
|Sample||TRPV6* ion channel in closed states|
|Function / homology||Ankyrin repeat / Ankyrin repeat region circular profile. / Ankyrin repeat profile. / Ankyrin repeats (3 copies) / Ion transport protein / Ankyrin repeat-containing domain superfamily / Transient receptor potential cation channel subfamily V / Ankyrin repeat-containing domain / Transient receptor potential channel, vanilloid 6 / Transient receptor potential cation channel subfamily V member 5/6 ...Ankyrin repeat / Ankyrin repeat region circular profile. / Ankyrin repeat profile. / Ankyrin repeats (3 copies) / Ion transport protein / Ankyrin repeat-containing domain superfamily / Transient receptor potential cation channel subfamily V / Ankyrin repeat-containing domain / Transient receptor potential channel, vanilloid 6 / Transient receptor potential cation channel subfamily V member 5/6 / Ion transport domain / in:ipr004729: / calcium activated cation channel activity / calcium ion import across plasma membrane / calcium ion import / calcium ion homeostasis / calcium channel activity / calcium ion transmembrane transport / response to calcium ion / protein homotetramerization / apical plasma membrane / calmodulin binding / integral component of plasma membrane / identical protein binding / plasma membrane / metal ion binding / Transient receptor potential cation channel subfamily V member 6|
Function and homology information
|Source||Rattus norvegicus (Norway rat)|
|Method||single particle reconstruction / cryo EM / 3.9 Å resolution|
|Authors||McGoldrick LL / Singh AK / Saotome K / Yelshanskaya MV / Twomey EC / Grassucci RA / Sobolevsky AI|
|Citation||Journal: Nature / Year: 2018|
Title: Opening of the human epithelial calcium channel TRPV6.
Authors: Luke L McGoldrick / Appu K Singh / Kei Saotome / Maria V Yelshanskaya / Edward C Twomey / Robert A Grassucci / Alexander I Sobolevsky
|Validation Report||PDB-ID: 6bob|
SummaryFull reportAbout validation report
|Date||Deposition: Nov 18, 2017 / Header (metadata) release: Dec 6, 2017 / Map release: Dec 20, 2017 / Last update: Jan 24, 2018|
|Structure viewer||EM map: |
Downloads & links
|File||emd_7123.map.gz (map file in CCP4 format, 33959 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 0.98 Å|
CCP4 map header:
-Entire TRPV6* ion channel in closed states
|Entire||Name: TRPV6* ion channel in closed states / Number of components: 2|
-Component #1: protein, TRPV6* ion channel in closed states
|Protein||Name: TRPV6* ion channel in closed states / Recombinant expression: No|
|Source||Species: Rattus norvegicus (Norway rat)|
|Source (engineered)||Expression System: HEK-293 cells|
-Component #2: protein, Transient receptor potential cation channel subfamily V ...
|Protein||Name: Transient receptor potential cation channel subfamily V member 6|
Recombinant expression: No
|Mass||Theoretical: 77.133492 kDa|
|Source (engineered)||Expression System: Rattus norvegicus (Norway rat)|
|Specimen||Specimen state: particle / Method: cryo EM|
|Sample solution||Specimen conc.: 0.5 mg/ml / Buffer solution: TRIS, NaCl, / pH: 8|
|Vitrification||Cryogen name: ETHANE / Temperature: 277 K / Humidity: 100 %|
-Electron microscopy imaging
Model: Tecnai F30 / Image courtesy: FEI Company
|Imaging||Microscope: FEI TECNAI F30|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 67 e/Å2 / Illumination mode: SPOT SCAN|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN K2 (4k x 4k)|
|Processing||Method: single particle reconstruction / Number of projections: 20808|
|3D reconstruction||Software: RELION / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF|
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