- EMDB-70444: Consensus reconstruction of the eukaryotic Ribosome-associated Qu... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-70444
タイトル
Consensus reconstruction of the eukaryotic Ribosome-associated Quality Control complex
マップデータ
試料
複合体: Ribosome-associated Quality Control complex from budding yeast
タンパク質・ペプチド: x 52種
RNA: x 4種
リガンド: x 5種
キーワード
ubiquitin / structural protein
機能・相同性
機能・相同性情報
: / alpha-aminoacyl-tRNA binding / RAS processing / Regulation of PTEN localization / : / UCH proteinases / CAT tailing / : / Aggrephagy / Pexophagy ...: / alpha-aminoacyl-tRNA binding / RAS processing / Regulation of PTEN localization / : / UCH proteinases / CAT tailing / : / Aggrephagy / Pexophagy / PINK1-PRKN Mediated Mitophagy / : / EGAD pathway / SCF complex disassembly in response to cadmium stress / mitotic DNA replication termination / Ovarian tumor domain proteases / Cdc48p-Npl4p-Vms1p AAA ATPase complex / Doa10p ubiquitin ligase complex / stress-induced homeostatically regulated protein degradation pathway / Peroxisomal protein import / : / protein localization to vacuole / endoplasmic reticulum membrane fusion / sister chromatid biorientation / Hrd1p ubiquitin ligase ERAD-L complex / DNA replication termination / RQC complex / : / Endosomal Sorting Complex Required For Transport (ESCRT) / mitochondria-associated ubiquitin-dependent protein catabolic process / peptide biosynthetic process / positive regulation of mitochondrial fusion / cytoplasm protein quality control by the ubiquitin-proteasome system / HSF1 activation / protein-containing complex disassembly / : / protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / nuclear protein quality control by the ubiquitin-proteasome system / endosome to plasma membrane protein transport / ribophagy / Translesion synthesis by REV1 / : / : / : / protein phosphatase regulator activity / piecemeal microautophagy of the nucleus / : / mating projection tip / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / : / Protein methylation / replisome / mitotic spindle disassembly / ribosome-associated ubiquitin-dependent protein catabolic process / VCP-NPL4-UFD1 AAA ATPase complex / pre-mRNA 5'-splice site binding / vesicle-fusing ATPase / cytosolic large ribosomal subunit assembly / K48-linked polyubiquitin modification-dependent protein binding / nuclear outer membrane-endoplasmic reticulum membrane network / nonfunctional rRNA decay / maturation of 5.8S rRNA / response to cycloheximide / Regulation of PTEN stability and activity / retrograde protein transport, ER to cytosol / CDK-mediated phosphorylation and removal of Cdc6 / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / KEAP1-NFE2L2 pathway / Neddylation / Formation of TC-NER Pre-Incision Complex / Ubiquitin-Mediated Degradation of Phosphorylated Cdc25A / Orc1 removal from chromatin / MAPK6/MAPK4 signaling / SRP-dependent cotranslational protein targeting to membrane / Gap-filling DNA repair synthesis and ligation in TC-NER / GTP hydrolysis and joining of the 60S ribosomal subunit / ribosomal large subunit binding / negative regulation of mRNA splicing, via spliceosome / preribosome, large subunit precursor / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / protein quality control for misfolded or incompletely synthesized proteins / Antigen processing: Ubiquitination & Proteasome degradation / L13a-mediated translational silencing of Ceruloplasmin expression / Dual incision in TC-NER / translational elongation / ribosomal large subunit export from nucleus / autophagosome maturation / 90S preribosome / Ub-specific processing proteases / mRNA transport / polyubiquitin modification-dependent protein binding / subtelomeric heterochromatin formation / ribosomal subunit export from nucleus / ATP metabolic process / regulation of translational fidelity / translational termination 類似検索 - 分子機能
TCF25/Rqc1 / Transcriptional repressor TCF25 / NFACT protein, C-terminal / NFACT protein C-terminal domain / E3 ubiquitin-protein ligase listerin / Listerin, zinc finger, RING-type / : / : / : / E3 ubiquitin-protein ligase listerin, N-terminal domain ...TCF25/Rqc1 / Transcriptional repressor TCF25 / NFACT protein, C-terminal / NFACT protein C-terminal domain / E3 ubiquitin-protein ligase listerin / Listerin, zinc finger, RING-type / : / : / : / E3 ubiquitin-protein ligase listerin, N-terminal domain / LTN1 family HEAT repeat region / Ubiquitin conjugating domain-like / FANCL C-terminal domain / : / NPL4, zinc-binding putative / NPL4 family, putative zinc binding region / Zinc finger, RING-CH-type / The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity. / Nuclear pore localisation protein NPL4, C-terminal / Nuclear protein localization protein 4 / NPL4 family / NFACT, RNA-binding domain / NFACT protein RNA binding domain / NFACT N-terminal and middle domains / Ring finger domain / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / AAA ATPase, CDC48 family / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / Cell division protein 48 (CDC48), N-terminal domain / : / : / CDC48, N-terminal subdomain / Cell division protein 48 (CDC48) N-terminal domain / CDC48, domain 2 / Cell division protein 48 (CDC48), domain 2 / Cell division protein 48 (CDC48) domain 2 / CDC48 domain 2-like superfamily / 50S ribosomal protein L10, insertion domain superfamily / : / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Aspartate decarboxylase-like domain superfamily / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L1 / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L19, eukaryotic / Ribosomal protein L10e, conserved site / : / Ribosomal protein L10e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / Ribosomal protein L44e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L10e / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / : / Ribosomal L40e family / Ribosomal protein L30e signature 1. / Ribosomal protein L36e signature. / Ribosomal protein L44e / Ribosomal protein L44 / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L35Ae, conserved site / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L35Ae signature. 類似検索 - ドメイン・相同性
Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein eL6B / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A ...Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein eL6B / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL22A / Large ribosomal subunit protein uL5A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Polyubiquitin / Polyubiquitin-C / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL1A / Large ribosomal subunit protein uL2A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein uL11A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL8A / AAA family ATPase involved in ubiquitin-mediated protein degradation CDC48 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL13A / Nuclear protein localization protein 4 / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL21A / E3 ubiquitin-protein ligase listerin / Ribosome quality control complex subunit 1 / Eukaryotic translation initiation factor 6 / Ribosome quality control complex subunit 2 / Large ribosomal subunit protein eL13A 類似検索 - 構成要素
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35 GM133772
米国
引用
ジャーナル: Nat Commun / 年: 2025 タイトル: Mechanism of nascent chain removal by the ribosome-associated quality control complex. 著者: Wenyan Li / Talia Scheel / Peter S Shen / 要旨: Errors during translation can cause ribosome stalling, leaving incomplete nascent chains attached to large ribosomal subunits. Cells rely on the Ribosome-associated Quality Control (RQC) complex to ...Errors during translation can cause ribosome stalling, leaving incomplete nascent chains attached to large ribosomal subunits. Cells rely on the Ribosome-associated Quality Control (RQC) complex to recognize, process, and remove these aberrant proteins to maintain proteostasis. Despite its importance, the mechanisms by which the RQC orchestrates nascent chain processing and extraction have remained unclear. Here, we present a cryo-EM structure of the RQC complex from budding yeast, revealing how its core components function in nascent chain removal. We show that the Cdc48 ATPase and its Ufd1-Npl4 adaptor are recruited by the Ltn1 E3 ubiquitin ligase to extract ubiquitylated peptides from the 60S ribosome. Additionally, we find that Rqc1 bridges the 60S subunit with ubiquitin and Ltn1, facilitating formation of K48-linked polyubiquitin chains. These findings provide a structural and mechanistic framework for understanding how the RQC complex collaborates to clear stalled translation products, advancing insight into cellular protein quality control.