EMDB-70444: Consensus reconstruction of the eukaryotic Ribosome-associated Qu...

Basic information

Entry

Database: EMDB / ID: EMD-70444

• Title: Consensus reconstruction of the eukaryotic Ribosome-associated Quality Control complex

Sample

• Complex: Ribosome-associated Quality Control complex from budding yeast
  • Protein or peptide: x 52 types
  • RNA: x 4 types
• Ligand: x 5 types

• Keywords: ubiquitin / structural protein

Function / homology

Function:

alpha-aminoacyl-tRNA binding / CAT tailing / EGAD pathway / SCF complex disassembly in response to cadmium stress / mitotic DNA replication termination / Ovarian tumor domain proteases / Cdc48p-Npl4p-Vms1p AAA ATPase complex / Doa10p ubiquitin ligase complex / protein localization to vacuole / stress-induced homeostatically regulated protein degradation pathway / endoplasmic reticulum membrane fusion / sister chromatid biorientation / Hrd1p ubiquitin ligase ERAD-L complex / DNA replication termination / RQC complex / mitochondria-associated ubiquitin-dependent protein catabolic process / protein-containing complex disassembly / peptide biosynthetic process / positive regulation of mitochondrial fusion / cytoplasm protein quality control by the ubiquitin-proteasome system / HSF1 activation / endosome to plasma membrane protein transport / protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / nuclear protein quality control by the ubiquitin-proteasome system / ribophagy / protein phosphatase regulator activity / piecemeal microautophagy of the nucleus / replisome / mating projection tip / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Protein methylation / mitotic spindle disassembly / VCP-NPL4-UFD1 AAA ATPase complex / Ribosome Quality Control (RQC) complex extracts and degrades nascent peptide / pre-mRNA 5'-splice site binding / vesicle-fusing ATPase / cytosolic large ribosomal subunit assembly / K48-linked polyubiquitin modification-dependent protein binding / PELO:HBS1L and ABCE1 dissociate a ribosome on a non-stop mRNA / nuclear outer membrane-endoplasmic reticulum membrane network / maturation of 5.8S rRNA / nonfunctional rRNA decay / KEAP1-NFE2L2 pathway / Neddylation / response to cycloheximide / retrograde protein transport, ER to cytosol / ribosome-associated ubiquitin-dependent protein catabolic process / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / ribosomal large subunit binding / Formation of a pool of free 40S subunits / preribosome, large subunit precursor / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Antigen processing: Ubiquitination & Proteasome degradation / L13a-mediated translational silencing of Ceruloplasmin expression / protein quality control for misfolded or incompletely synthesized proteins / negative regulation of mRNA splicing, via spliceosome / subtelomeric heterochromatin formation / translational elongation / autophagosome maturation / ribosomal large subunit export from nucleus / protein unfolding / mRNA transport / polyubiquitin modification-dependent protein binding / 90S preribosome / ribosomal subunit export from nucleus / ATP metabolic process / translational termination / regulation of translational fidelity / maturation of LSU-rRNA / protein-RNA complex assembly / ERAD pathway / Neutrophil degranulation / translation initiation factor activity / protein catabolic process / rescue of stalled cytosolic ribosome / macroautophagy / cytosolic ribosome assembly / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / assembly of large subunit precursor of preribosome / cytosolic ribosome / ubiquitin binding / proteasomal protein catabolic process / translational initiation / positive regulation of protein localization to nucleus / RING-type E3 ubiquitin transferase / maintenance of translational fidelity / modification-dependent protein catabolic process / cytoplasmic stress granule / protein tag activity / ubiquitin-protein transferase activity / rRNA processing / ubiquitin protein ligase activity / peroxisome / nuclear membrane / chromatin organization / ribosome biogenesis

Domain/homology:

TCF25/Rqc1 / Transcriptional repressor TCF25 / NFACT protein, C-terminal / NFACT protein C-terminal domain / E3 ubiquitin-protein ligase listerin / Listerin, zinc finger, RING-type / : / : / : / E3 ubiquitin-protein ligase listerin, N-terminal domain / LTN1 family HEAT repeat region / Ubiquitin conjugating domain-like / FANCL C-terminal domain / : / NPL4, zinc-binding putative / NPL4 family, putative zinc binding region / Zinc finger, RING-CH-type / The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity. / Nuclear pore localisation protein NPL4, C-terminal / Nuclear protein localization protein 4 / NPL4 family / NFACT, RNA-binding domain / NFACT protein RNA binding domain / NFACT N-terminal and middle domains / Ring finger domain / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / AAA ATPase, CDC48 family / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / Cell division protein 48 (CDC48), N-terminal domain / : / : / CDC48, N-terminal subdomain / Cell division protein 48 (CDC48) N-terminal domain / CDC48, domain 2 / Cell division protein 48 (CDC48), domain 2 / Cell division protein 48 (CDC48) domain 2 / CDC48 domain 2-like superfamily / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / : / Aspartate decarboxylase-like domain superfamily / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L1 / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L13e / Ribosomal protein L13e / : / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L6e signature. / Ribosomal protein L19, eukaryotic / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L44e signature. / Ribosomal protein L24e, conserved site / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein L10e / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal L40e family / Ribosomal protein L36e signature. / Ribosomal protein L30e signature 1. / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal Protein L6, KOW domain / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / : / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature.

Component:

Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein eL6B / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL22A / Large ribosomal subunit protein uL5A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Polyubiquitin / Polyubiquitin-C / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL1A / Large ribosomal subunit protein uL2A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein uL11A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL8A / AAA family ATPase involved in ubiquitin-mediated protein degradation CDC48 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL13A / Nuclear protein localization protein 4 / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL21A / E3 ubiquitin-protein ligase listerin / Ribosome quality control complex subunit 1 / Eukaryotic translation initiation factor 6 / Ribosome quality control complex subunit 2 / Large ribosomal subunit protein eL13A

• Biological species: Saccharomyces cerevisiae (brewer's yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 3.16 Å
• Authors: Li W / Cahoon T / Shen PS

Funding support

United States, 1 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R35 GM133772	United States

• Citation: Journal: Nat Commun / Year: 2025; Title: Mechanism of nascent chain removal by the ribosome-associated quality control complex.; Authors: Wenyan Li / Talia Scheel / Peter S Shen / ; Abstract: Errors during translation can cause ribosome stalling, leaving incomplete nascent chains attached to large ribosomal subunits. Cells rely on the Ribosome-associated Quality Control (RQC) complex to recognize, process, and remove these aberrant proteins to maintain proteostasis. Despite its importance, the mechanisms by which the RQC orchestrates nascent chain processing and extraction have remained unclear. Here, we present a cryo-EM structure of the RQC complex from budding yeast, revealing how its core components function in nascent chain removal. We show that the Cdc48 ATPase and its Ufd1-Npl4 adaptor are recruited by the Ltn1 E3 ubiquitin ligase to extract ubiquitylated peptides from the 60S ribosome. Additionally, we find that Rqc1 bridges the 60S subunit with ubiquitin and Ltn1, facilitating formation of K48-linked polyubiquitin chains. These findings provide a structural and mechanistic framework for understanding how the RQC complex collaborates to clear stalled translation products, advancing insight into cellular protein quality control.

History

Deposition	Apr 30, 2025	-
Header (metadata) release	Jun 11, 2025	-
Map release	Jun 11, 2025	-
Update	Jul 16, 2025	-
Current status	Jul 16, 2025	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_70444.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.058 Å

Density

Contour Level	By EMDB: 0.187
Minimum - Maximum	-0.7877744 - 1.5529041
Average (Standard dev.)	0.00043592646 (±0.050965346)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 640 640 640 Spacing 640 640 640
Cell	A=B=C: 677.12 Å α=β=γ: 90.0 °

Supplemental data

Half map: #1

• File: emd_70444_half_map_1.map

Half map: #2

• File: emd_70444_half_map_2.map

Sample components

Entire : Ribosome-associated Quality Control complex from budding yeast

• Entire: Name: Ribosome-associated Quality Control complex from budding yeast

Components

• Complex: Ribosome-associated Quality Control complex from budding yeast
  • Protein or peptide: Cell division control protein 48
  • Protein or peptide: Ubiquitin
  • Protein or peptide: Nuclear protein localization protein 4
  • Protein or peptide: 60S ribosomal protein L17-A
  • Protein or peptide: 60S ribosomal protein L18-A
  • Protein or peptide: 60S ribosomal protein L19-A
  • Protein or peptide: 60S ribosomal protein L20-A
  • Protein or peptide: 60S ribosomal protein L21-A
  • Protein or peptide: 60S ribosomal protein L22-A
  • Protein or peptide: 60S ribosomal protein L23-A
  • Protein or peptide: Large ribosomal subunit protein eL24A
  • Protein or peptide: 60S ribosomal protein L26-A
  • Protein or peptide: 60S ribosomal protein L27-A
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: Large ribosomal subunit protein eL29
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31-A
  • Protein or peptide: Large ribosomal subunit protein eL32
  • Protein or peptide: 60S ribosomal protein L33-A
  • Protein or peptide: 60S ribosomal protein L34-A
  • Protein or peptide: 60S ribosomal protein L35-A
  • Protein or peptide: 60S ribosomal protein L36-A
  • Protein or peptide: 60S ribosomal protein L37-A
  • Protein or peptide: 60S ribosomal protein L39
  • Protein or peptide: Ubiquitin-60S ribosomal protein L40
  • Protein or peptide: 60S ribosomal protein L42-A
  • Protein or peptide: 60S ribosomal protein L43-A
  • Protein or peptide: 60S ribosomal protein L41-A
  • RNA: 25S rRNA
  • RNA: 5S rRNA
  • RNA: 5.8S rRNA
  • Protein or peptide: 60S ribosomal protein L2-A
  • Protein or peptide: Large ribosomal subunit protein uL3
  • Protein or peptide: Large ribosomal subunit protein uL4A
  • Protein or peptide: Large ribosomal subunit protein uL18
  • Protein or peptide: Large ribosomal subunit protein eL6B
  • Protein or peptide: 60S ribosomal protein L7-A
  • Protein or peptide: 60S ribosomal protein L8-A
  • Protein or peptide: 60S ribosomal protein L9-A
  • Protein or peptide: Large ribosomal subunit protein uL16
  • Protein or peptide: Large ribosomal subunit protein uL5A
  • Protein or peptide: 60S ribosomal protein L13-A
  • Protein or peptide: 60S ribosomal protein L14-A
  • Protein or peptide: Ribosome quality control complex subunit 2
  • Protein or peptide: E3 ubiquitin-protein ligase listerin
  • Protein or peptide: Eukaryotic translation initiation factor 6
  • RNA: Ala tRNA
  • Protein or peptide: Large ribosomal subunit protein uL11A
  • Protein or peptide: 60S acidic ribosomal protein P0
  • Protein or peptide: CAT-tailed nascent peptide
  • Protein or peptide: Large ribosomal subunit protein uL1A
  • Protein or peptide: Large ribosomal subunit protein eL38
  • Protein or peptide: Ribosome quality control complex subunit 1
  • Protein or peptide: Polyubiquitin-C
  • Protein or peptide: 60S ribosomal protein L16-A
  • Protein or peptide: 60S ribosomal protein L25
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: ADENOSINE-5'-DIPHOSPHATE
• Ligand: ZINC ION
• Ligand: MAGNESIUM ION
• Ligand: SPERMIDINE

Supramolecule #1: Ribosome-associated Quality Control complex from budding yeast

• Supramolecule: Name: Ribosome-associated Quality Control complex from budding yeast; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#56
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Macromolecule #1: Cell division control protein 48

• Macromolecule: Name: Cell division control protein 48 / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO / EC number: vesicle-fusing ATPase
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 92.105922 KDa

Sequence

String:

MGEEHKPLLD ASGVDPREED KTATAILRRK KKDNMLLVDD AINDDNSVIA INSNTMDKLE LFRGDTVLVK GKKRKDTVLI VLIDDELED GACRINRVVR NNLRIRLGDL VTIHPCPDIK YATRISVLPI ADTIEGITGN LFDVFLKPYF VEAYRPVRKG D HFVVRGGM RQVEFKVVDV EPEEYAVVAQ DTIIHWEGEP INREDEENNM NEVGYDDIGG CRKQMAQIRE MVELPLRHPQ LF KAIGIKP PRGVLMYGPP GTGKTLMARA VANETGAFFF LINGPEVMSK MAGESESNLR KAFEEAEKNA PAIIFIDEID SIA PKRDKT NGEVERRVVS QLLTLMDGMK ARSNVVVIAA TNRPNSIDPA LRRFGRFDRE VDIGIPDATG RLEVLRIHTK NMKL ADDVD LEALAAETHG YVGADIASLC SEAAMQQIRE KMDLIDLDED EIDAEVLDSL GVTMDNFRFA LGNSNPSALR ETVVE SVNV TWDDVGGLDE IKEELKETVE YPVLHPDQYT KFGLSPSKGV LFYGPPGTGK TLLAKAVATE VSANFISVKG PELLSM WYG ESESNIRDIF DKARAAAPTV VFLDQLDSIA KARGGSLGDA GGASDRVVNQ LLTEMDGMNA KKNVFVIGAT NRPDQID PA ILRPGRLDQL IYVPLPDENA RLSILNAQLR KTPLEPGLEL TAIAKATQGF SGADLLYIVQ RAAKYAIKDS IEAHRQHE A EKEVKVEGED VEMTDEGAKA EQEPEVDPVP YITKEHFAEA MKTAKRSVSD AELRRYEAYS QQMKASRGQF SNFNFNDAP LGTTATDNAN SNNSAPSGAG AAFGSNAEED DDLYS

UniProtKB: AAA family ATPase involved in ubiquitin-mediated protein degradation CDC48

Macromolecule #2: Ubiquitin

• Macromolecule: Name: Ubiquitin / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.568769 KDa

Sequence

String:

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

UniProtKB: Polyubiquitin

Macromolecule #3: Nuclear protein localization protein 4

• Macromolecule: Name: Nuclear protein localization protein 4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 65.862062 KDa

Sequence

String:

MLIRFRSKNG THRVSCQEND LFGTVIEKLV GNLDPNADVD TFTVCEKPGQ GIHAVSELAD RTVMDLGLKH GDMLILNYSD KPANEKDGV NVEIGSVGID SKGIRQHRYG PLRIKELAVD EELEKEDGLI PRQKSKLCKH GDRGMCEYCS PLPPWDKEYH E KNKIKHIS FHSYLKKLNE NANKKENGSS YISPLSEPDF RINKRCHNGH EPWPRGICSK CQPSAITLQQ QEFRMVDHVE FQ KSEIINE FIQAWRYTGM QRFGYMYGSY SKYDNTPLGI KAVVEAIYEP PQHDEQDGLT MDVEQVKNEM LQIDRQAQEM GLS RIGLIF TDLSDAGAGD GSVFCKRHKD SFFLSSLEVI MAARHQTRHP NVSKYSEQGF FSSKFVTCVI SGNLEGEIDI SSYQ VSTEA EALVTADMIS GSTFPSMAYI NDTTDERYVP EIFYMKSNEY GITVKENAKP AFPVDYLLVT LTHGFPNTDT ETNSK FVSS TGFPWSNRQA MGQSQDYQEL KKYLFNVASS GDFNLLHEKI SNFHLLLYIN SLQILSPDEW KLLIESAVKN EWEESL LKL VSSAGWQTLV MILQESG

UniProtKB: Nuclear protein localization protein 4

Macromolecule #4: 60S ribosomal protein L17-A

• Macromolecule: Name: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.589518 KDa

Sequence

String:

MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLEQ VLDHQRAIPF RRFNSSIGRT AQGKEFGVTK ARWPAKSVK FVQGLLQNAA ANAEAKGLDA TKLYVSHIQV NQAPKQRRRT YRAHGRINKY ESSPSHIELV VTEKEEAVAK A AEKKVVRL TSRQRGRIAA QKRIAA

UniProtKB: Large ribosomal subunit protein uL22A

Macromolecule #5: 60S ribosomal protein L18-A

• Macromolecule: Name: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.609252 KDa

Sequence

String:

MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTD DARIFEFPKT TVAALRFTAG ARAKIVKAGG ECITLDQLAV RAPKGQNTLI LRGPRNSREA VRHFGMGPHK G KAPRILST GRKFERARGR RRSKGFKV

UniProtKB: Large ribosomal subunit protein eL18A

Macromolecule #6: 60S ribosomal protein L19-A

• Macromolecule: Name: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.762316 KDa

Sequence

String:

MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI VKKAVTVHSK SRTRAHAQSK REGRHSGYGK RKGTREARL PSQVVWIRRL RVLRRLLAKY RDAGKIDKHL YHVLYKESKG NAFKHKRALV EHIIQAKADA QREKALNEEA E ARRLKNRA ARDRRAQRVA EKRDALLKED A

UniProtKB: Large ribosomal subunit protein eL19A

Macromolecule #7: 60S ribosomal protein L20-A

• Macromolecule: Name: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.478852 KDa

Sequence

String:

MAHFKEYQVI GRRLPTESVP EPKLFRMRIF ASNEVIAKSR YWYFLQKLHK VKKASGEIVS INQINEAHPT KVKNFGVWVR YDSRSGTHN MYKEIRDVSR VAAVETLYQD MAARHRARFR SIHILKVAEI EKTADVKRQY VKQFLTKDLK FPLPHRVQKS T KTFSYKRP STFY

UniProtKB: Large ribosomal subunit protein eL20A

Macromolecule #8: 60S ribosomal protein L21-A

• Macromolecule: Name: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 18.279266 KDa

Sequence

String:

MGKSHGYRSR TRYMFQRDFR KHGAVHLSTY LKVYKVGDIV DIKANGSIQK GMPHKFYQGK TGVVYNVTKS SVGVIINKMV GNRYLEKRL NLRVEHIKHS KCRQEFLERV KANAAKRAEA KAQGVAVQLK RQPAQPRESR IVSTEGNVPQ TLAPVPYETF I

UniProtKB: Large ribosomal subunit protein eL21A

Macromolecule #9: 60S ribosomal protein L22-A

• Macromolecule: Name: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.711359 KDa

Sequence

String:

MAPNTSRKQK IAKTFTVDVS SPTENGVFDP ASYAKYLIDH IKVEGAVGNL GNAVTVTEDG TVVTVVSTAK FSGKYLKYLT KKYLKKNQL RDWIRFVSTK TNEYRLAFYQ VTPEEDEEED EE

UniProtKB: Large ribosomal subunit protein eL22A

Macromolecule #10: 60S ribosomal protein L23-A

• Macromolecule: Name: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.49395 KDa

Sequence

String:

MSGNGAQGTK FRISLGLPVG AIMNCADNSG ARNLYIIAVK GSGSRLNRLP AASLGDMVMA TVKKGKPELR KKVMPAIVVR QAKSWRRRD GVFLYFEDNA GVIANPKGEM KGSAITGPVG KECADLWPRV ASNSGVVV

UniProtKB: Large ribosomal subunit protein uL14A

Macromolecule #11: Large ribosomal subunit protein eL24A

• Macromolecule: Name: Large ribosomal subunit protein eL24A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 17.661717 KDa

Sequence

String:

MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITEEVAKK RSRKTVKAQR PITGASLDL IKERRSLKPE VRKANREEKL KANKEKKKAE KAARKAEKAK SAGTQSSKFS KQQAKGAFQK VAATSR

UniProtKB: Large ribosomal subunit protein eL24A

Macromolecule #12: 60S ribosomal protein L26-A

• Macromolecule: Name: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.265784 KDa

Sequence

String:

MAKQSLDVSS DRRKARKAYF TAPSSQRRVL LSAPLSKELR AQYGIKALPI RRDDEVLVVR GSKKGQEGKI SSVYRLKFAV QVDKVTKEK VNGASVPINL HPSKLVITKL HLDKDRKALI QRKGGKLE

UniProtKB: Large ribosomal subunit protein uL24A

Macromolecule #13: 60S ribosomal protein L27-A

• Macromolecule: Name: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.56836 KDa

Sequence

String:

MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP LKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDV EAFKSVVSTE TFEQPSQREE AKKVVKKAFE ERHQAGKNQW FFSKLRF

UniProtKB: Large ribosomal subunit protein eL27A

Macromolecule #14: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.761666 KDa

Sequence

String:

MPSRFTKTRK HRGHVSAGKG RIGKHRKHPG GRGMAGGQHH HRINMDKYHP GYFGKVGMRY FHKQQAHFWK PVLNLDKLWT LIPEDKRDQ YLKSASKETA PVIDTLAAGY GKILGKGRIP NVPVIVKARF VSKLAEEKIR AAGGVVELIA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #15: Large ribosomal subunit protein eL29

• Macromolecule: Name: Large ribosomal subunit protein eL29 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.691884 KDa

Sequence

String:

MAKSKNHTAH NQTRKAHRNG IKKPKTYKYP SLKGVDPKFR RNHKHALHGT AKALAAAKK

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #16: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.430364 KDa

Sequence

String:

MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGV VSILEAGDSD ILTTLA

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #17: 60S ribosomal protein L31-A

• Macromolecule: Name: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.980158 KDa

Sequence

String:

MAGLKDVVTR EYTINLHKRL HGVSFKKRAP RAVKEIKKFA KLHMGTDDVR LAPELNQAIW KRGVKGVEYR LRLRISRKRN EEEDAKNPL FSYVEPVLVA SAKGLQTVVV EEDA

UniProtKB: Large ribosomal subunit protein eL31A

Macromolecule #18: Large ribosomal subunit protein eL32

• Macromolecule: Name: Large ribosomal subunit protein eL32 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.809441 KDa

Sequence

String:

MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHT KTYAAEIAHN ISAKNRVVIL ARAKALGIKV TNPKGRLALE A

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #19: 60S ribosomal protein L33-A

• Macromolecule: Name: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.17713 KDa

Sequence

String:

MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNL PAKTFGASVR IFLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

Macromolecule #20: 60S ribosomal protein L34-A

• Macromolecule: Name: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.673196 KDa

Sequence

String:

MAQRVTFRRR NPYNTRSNKI KVVKTPGGIL RAQHVKKLAT RPKCGDCGSA LQGISTLRPR QYATVSKTHK TVSRAYGGSR CANCVKERI IRAFLIEEQK IVKKVVKEQT EAAKKSEKKA KK

UniProtKB: Large ribosomal subunit protein eL34A

Macromolecule #21: 60S ribosomal protein L35-A

• Macromolecule: Name: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.94264 KDa

Sequence

String:

MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALR RALTKFEASQ VTEKQRKKQI AFPQRKYAIK A

UniProtKB: Large ribosomal subunit protein uL29A

Macromolecule #22: 60S ribosomal protein L36-A

• Macromolecule: Name: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.151259 KDa

Sequence

String:

MTVKTGIAIG LNKGKKVTSM TPAPKISYKK GAASNRTKFV RSLVREIAGL SPYERRLIDL IRNSGEKRAR KVAKKRLGSF TRAKAKVEE MNNIIAASRR H

UniProtKB: Large ribosomal subunit protein eL36A

Macromolecule #23: 60S ribosomal protein L37-A

• Macromolecule: Name: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.877395 KDa

Sequence

String:

MGKGTPSFGK RHNKSHTLCN RCGRRSFHVQ KKTCSSCGYP AAKTRSYNWG AKAKRRHTTG TGRMRYLKHV SRRFKNGFQT GSASKASA

UniProtKB: Large ribosomal subunit protein eL37A

Macromolecule #24: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.35864 KDa

Sequence

String:

MAAQKSFRIK QKMAKAKKQN RPLPQWIRLR TNNTIRYNAK RRNWRRTKMN I

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #25: Ubiquitin-60S ribosomal protein L40

• Macromolecule: Name: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.583077 KDa

Sequence

String:

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLKALASKY NCDKSVCRKC YARLPPRATN CRKRKCGHTN QLRPKKKLK

UniProtKB: Ubiquitin-ribosomal protein eL40A fusion protein

Macromolecule #26: 60S ribosomal protein L42-A

• Macromolecule: Name: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.246658 KDa

Sequence

String:

MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF GGQTKPVFHK KAKTTKKVVL RLECVKCKTR AQLTLKRCK HFELGGEKKQ KGQALQF

UniProtKB: Large ribosomal subunit protein eL42A

Macromolecule #27: 60S ribosomal protein L43-A

• Macromolecule: Name: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 10.112952 KDa

Sequence

String:

MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE IQQHARYDCS FCGKKTVKRG AAGIWTCSCC KKTVAGGAYT VSTAAAATVR STIRRLREM VEA

UniProtKB: Large ribosomal subunit protein eL43A

Macromolecule #28: 60S ribosomal protein L41-A

• Macromolecule: Name: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 3.354243 KDa

Sequence

String:

MRAKWRKKRT RRLKRKRRKV RARSK

UniProtKB: Small ribosomal subunit protein eS32A

Macromolecule #32: 60S ribosomal protein L2-A

• Macromolecule: Name: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.463574 KDa

Sequence

String:

MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIY AGKKASLNVG NVLPLGSVPE GTIVSNVEEK PGDRGALARA SGNYVIIIGH NPDENKTRVR LPSGAKKVIS S DARGVIGV IAGGGRVDKP LLKAGRAFHK YRLKRNSWPK TRGVAMNPVD HPHGGGNHQH IGKASTISRG AVSGQKAGLI AA RRTGLLR GSQKTQD

UniProtKB: Large ribosomal subunit protein uL2A

Macromolecule #33: Large ribosomal subunit protein uL3

• Macromolecule: Name: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 43.850793 KDa

Sequence

String:

MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK TPLAQKKAHL AEIQLNGGSI SEKVDWAREH FEKTVAVDSV FEQNEMIDAI AVTKGHGFEG VTHRWGTKKL PR KTHRGLR KVACIGAWHP AHVMWSVARA GQRGYHSRTS INHKIYRVGK GDDEANGATS FDRTKKTITP MGGFVHYGEI KND FIMVKG CIPGNRKRIV TLRKSLYTNT SRKALEEVSL KWIDTASKFG KGRFQTPAEK HAFMGTLKKD L

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #34: Large ribosomal subunit protein uL4A

• Macromolecule: Name: Large ribosomal subunit protein uL4A / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 39.159125 KDa

Sequence

String:

MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA LKAVGAHSDL LKVLKSKKLR AGKGKYRNRR WTQRRGPLVV YAEDNGIVKA LRNVPGVETA NVASLNLLQL AP GAHLGRF VIWTEAAFTK LDQVWGSETV ASSKVGYTLP SHIISTSDVT RIINSSEIQS AIRPAGQATQ KRTHVLKKNP LKN KQVLLR LNPYAKVFAA EKLGSKKAEK TGTKPAAVFT ETLKHD

UniProtKB: Large ribosomal subunit protein uL4A

Macromolecule #35: Large ribosomal subunit protein uL18

• Macromolecule: Name: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 33.764828 KDa

Sequence

String:

MAFQKDAKSS AYSSRFQTPF RRRREGKTDY YQRKRLVTQH KAKYNTPKYR LVVRFTNKDI ICQIISSTIT GDVVLAAAYS HELPRYGIT HGLTNWAAAY ATGLLIARRT LQKLGLDETY KGVEEVEGEY ELTEAVEDGP RPFKVFLDIG LQRTTTGARV F GALKGASD GGLYVPHSEN RFPGWDFETE EIDPELLRSY IFGGHVSQYM EELADDDEER FSELFKGYLA DDIDADSLED IY TSAHEAI RADPAFKPTE KKFTKEQYAA ESKKYRQTKL SKEERAARVA AKIAALAGQQ

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #36: Large ribosomal subunit protein eL6B

• Macromolecule: Name: Large ribosomal subunit protein eL6B / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.024541 KDa

Sequence

String:

MTAQQAPKWY PSEDVAAPKK TRKAVRPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED NTLLVTGPFK VNGVPLRRVN ARYVIATST KVSVEGVNVE KFNVEYFAKE KLTKKEKKEA NLFPEQQTKE IKTERVEDQK VVDKALLAEI KKTPLLKQYL S ASFSLKNG DKPHLLKF

UniProtKB: Large ribosomal subunit protein eL6B

Macromolecule #37: 60S ribosomal protein L7-A

• Macromolecule: Name: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.686281 KDa

Sequence

String:

MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI IEANLGKYGI LSIDDLIHEI ITVGPHFKQA NNFLWPFKLS NPSGGWGVPR KFKHFIQGGS FGNREEFINK LV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

Macromolecule #38: 60S ribosomal protein L8-A

• Macromolecule: Name: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 28.17582 KDa

Sequence

String:

MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA LCKKMGVPYA IVKGKARLGT LVNQKTSAVA ALTEVRAEDE AALAKLVSTI DANFADKYDE VKKHWGGGIL GN KAQAKMD KRAKNSDSA

UniProtKB: Large ribosomal subunit protein eL8A

Macromolecule #39: 60S ribosomal protein L9-A

• Macromolecule: Name: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.605061 KDa

Sequence

String:

MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

UniProtKB: Large ribosomal subunit protein uL6A

Macromolecule #40: Large ribosomal subunit protein uL16

• Macromolecule: Name: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.410465 KDa

Sequence

String:

MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL SSEALEAARI CANKYMTTVS GRDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQQGMRGAW GKPHGLAARV DIGQIIFSVR TKDSNKDVVV EGLRRARYKF P GQQKIILS KKWGFTNLDR PEYLKKREAG EVKDDGAFVK FLSKKGSLEN NIREFPEYFA AQA

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #41: Large ribosomal subunit protein uL5A

• Macromolecule: Name: Large ribosomal subunit protein uL5A / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 19.755691 KDa

Sequence

String:

MSAKAQNPMR DLKIEKLVLN ISVGESGDRL TRASKVLEQL SGQTPVQSKA RYTVRTFGIR RNEKIAVHVT VRGPKAEEIL ERGLKVKEY QLRDRNFSAT GNFGFGIDEH IDLGIKYDPS IGIFGMDFYV VMNRPGARVT RRKRCKGTVG NSHKTTKEDT V SWFKQKYD ADVLDK

UniProtKB: Large ribosomal subunit protein uL5A

Macromolecule #42: 60S ribosomal protein L13-A

• Macromolecule: Name: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.604164 KDa

Sequence

String:

MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR TLRLARSEKK FRGIREKRAR EKAEAEAEKK K

UniProtKB: Large ribosomal subunit protein eL13A

Macromolecule #43: 60S ribosomal protein L14-A

• Macromolecule: Name: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.195066 KDa

Sequence

String:

MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAA VCEKWAASSW AKKIAQRERR AALTDFERFQ VMVLRKQKRY TVKKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

Macromolecule #44: Ribosome quality control complex subunit 2

• Macromolecule: Name: Ribosome quality control complex subunit 2 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 119.250234 KDa

Sequence

String:

MKQRISALDL LLLARELKQD LEGYRLSNIY NIADSSKQFL LKFNKPDSKL NVVVDCGLRI YLTEFSRPIP PTPSGFVVKL RKHLKAKRL TALKQVDQDR ILVLQFADGH FYLVLEFFSA GNVILLDENR RIMALQRVVL EHENKVGQIY EMFDESLFTT N NESADESI EKNRKAEYTS ELVNEWIKAV QAKYESDITV IKQLNIQGKE GAKKKKVKVP SIHKLLLSKV PHLSSDLLSK NL KVFNIDP SESCLNLLEE TDSLAELLNS TQLEYNQLLT TTDRKGYILA KRNENYISEK DTADLEFIYD TFHPFKPYIN GGD TDSSCI IEVEGPYNRT LDKFFSTIES SKYALRIQNQ ESQAQKKIDD ARAENDRKIQ ALLDVQELNE RKGHLIIENA PLIE EVKLA VQGLIDQQMD WNTIEKLIKS EQKKGNRIAQ LLNLPLNLKQ NKISVKLDLS SKELNTSSDE DNESEGNTTD SSSDS DSED MESSKERSTK SMKRKSNEKI NVTIDLGLSA YANATEYFNI KKTSAQKQKK VEKNVGKAMK NIEVKIDQQL KKKLKD SHS VLKKIRTPYF FEKYSWFISS EGFLVMMGKS PAETDQIYSK YIEDDDIYMS NSFNSHVWIK NPEKTEVPPN TLMQAGI LC MSSSEAWSKK ISSSPWWCFA KNVSKFDGSD NSILPEGAFR LKNENDQNHL PPAQLVMGFG FLWKVKTSGN EDNGDDDE E EEEEEEEEEE EEEEEEEEEE EEKEEEEKEE EQQQDEDDSN EVNGLEKGGD SNDSTKNNSF EHDNLEKDIE KHCTISSDT DSDSGNAKAK NDNSSTQRIL DEPGVPISLI ENINSNVRGK RGKLKKIQKK YADQDETERL LRLEALGTLK GIEKQQQRKK EEIMKREVR EDRKNKREKQ RRLQALKFTK KEKARVNYDK HKSELKPSLD KGDVVDDIIP VFAPWPALLK YKYKVKIQPG S AKKTKTLT EILHYFKSRP LDGSSTDNEM DWPQEHEMIK GLKEQDLVLL LCVDKLKVTI AGQKSTKNGG NSSKKGKKKR

UniProtKB: Ribosome quality control complex subunit 2

Macromolecule #45: E3 ubiquitin-protein ligase listerin

• Macromolecule: Name: E3 ubiquitin-protein ligase listerin / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 180.372984 KDa

Sequence

String:

MSFGGINTFQ QYNTDLGLGH NGVRISLNYF DGLPDPSLLN SLYSNELKLI FKSLLKRDET TKEKALMDLS NLISDFNQNE YFFNDIFLL CWSQIYAKLI ISDYKVIRLQ SHQITIMLVK SLRKKISKFL KDFIPLILLG TCELDYSVSK PSLNELTECF N KDPAKINA LWAVFQEQLL NLVKEIVVNE NEDTISDERY SSKEESEFRY HRVIASAVLL LIKLFVHNKD VSERNSSSLK VI LSDESIW KLLNLKNGQN TNAYETVLRL IDVLYTRGYM PSHKNIMKLA VKKLLKSLTH ITSKNILKVC PVLPSILNLL ATL DDYEDG TIWSYDKSSK EKVLKFLSVS RTSPSPGFFN AVFALYSSTK RHSFLDYYLE WLPFWQKSVQ RLNEKGFSAR NSAE VLNEF WTNFLKFAED SSEERVKKMV ESEIFNSLSC GKSLSEYTKL NQTLSGVFPP DKWEREIEDY FTSDEDIRKI KVSFE KNLF ALLVTSPNNE SAISRLFDFF VQLIETDPSN VFNKYDGVYD ALNYFLDSDM IFLNGKIGKF INEIPTLVQE STYQNF AGI MAQYSNSKFF KMNTDAITSL EDFFIVALSF NLPKTIILAT MNELDNDIYQ QLMKSDSLEL ELYIEDFMKN YKFDDSG EI FKGNNKFLNQ RTITTLYRSA VANGQVEQFC AVLSKLDETF FSTLLLNTDF LSCALYEVSE DTNEKLFKLS LQLAKGNS E IANKLAQVIL QHAQVYFSPG AKEKYVTHAV ELINGCNDTS QIFFPANAIE VFARYMPAID YRSSLVSSLS TNTHLLLTD DKPINLKNMQ KLIRYALFLD ALLDALPERV NNHIVAFITV VSELVTDYNC LSEEPNDLYY DFGHTFFKHG KVNLNFSDIV GNVIQPANG GDAMLTFDIA ESNSVYFFYY SRVLYKVLLN SIDTVSSTTL NGLLASVESF VTKTVRDQKS TDKDYLLCAI L LLMFNRSN SKDEITKLRT LLASQLIGIR EVELVDQEFK SLALLNNLLD IPQADKQFVP IAPQRLNMIF RSILKWLDSD LA YEPSFST VRLLLLDFFT KLMRFEGVRD MGITAFELSE RLLADSLSMC QIDDTLYLLE LRSSCLNLYE TLSQGVSKNG EEI SEYGDE IQENLIELMF LNFNQERNNQ VSTLFYQKLY KVISSMELKK LESQYKRIFE VVLNDKDIGS NINQSRLLTT LLGS LVVKT QQDIIIEYEL RIQKQTGSDV DGSASDNDVN SKFKLPQKLL QKVTDEVPKE YLEYENKNSF IKYLWYWHLI LMYFK DTSY NMRQIFIEQL KEAGLINRMF DFITDQIDLR DTEFWKQVDT KEISEYNIVG NNFSPYKEDI FEECKKLLGH TLYQLF NNV GCLTSIWWLN IKDRTLQNDI EKFVSEFISP ILIKNEFDDI NSKMDRLTSN DDALTIKLNN ITNEVKASYL IDDQKLE IS FKLPKNYPLT NIQVNGVSRV GISEQKWKQW IMSTQHVITG MNGSVLDSLE LFTKNVHLQF SGFEECAICY SILHAVDR K LPSKTCPTCK NKFHGACLYK WFRSSGNNTC PLCRSEIPFR R

UniProtKB: E3 ubiquitin-protein ligase listerin

Macromolecule #46: Eukaryotic translation initiation factor 6

• Macromolecule: Name: Eukaryotic translation initiation factor 6 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 26.476605 KDa

Sequence

String:

MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLP DSVKIQRVEE RLSALGNVIC CNDYVALVHP DIDRETEELI SDVLGVEVFR QTISGNILVG SYCSLSNQGG L VHPQTSVQ DQEELSSLLQ VPLVAGTVNR GSSVVGAGMV VNDYLAVTGL DTTAPELSVI ESIFRLQDAQ PESISGNLRD TL IETYS

UniProtKB: Eukaryotic translation initiation factor 6

Macromolecule #48: Large ribosomal subunit protein uL11A

• Macromolecule: Name: Large ribosomal subunit protein uL11A / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 17.850621 KDa

Sequence

String:

MPPKFDPNEV KYLYLRAVGG EVGASAALAP KIGPLGLSPK KVGEDIAKAT KEFKGIKVTV QLKIQNRQAA ASVVPSASSL VITALKEPP RDRKKDKNVK HSGNIQLDEI IEIARQMRDK SFGRTLASVT KEILGTAQSV GCRVDFKNPH DIIEGINAGE I EIPEN

UniProtKB: Large ribosomal subunit protein uL11A

Macromolecule #49: 60S acidic ribosomal protein P0

• Macromolecule: Name: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 33.749121 KDa

Sequence

String:

MGGIREKKAE YFAKLREYLE EYKSLFVVGV DNVSSQQMHE VRKELRGRAV VLMGKNTMVR RAIRGFLSDL PDFEKLLPFV KGNVGFVFT NEPLTEIKNV IVSNRVAAPA RAGAVAPEDI WVRAVNTGME PGKTSFFQAL GVPTKIARGT IEIVSDVKVV D AGNKVGQS EASLLNLLNI SPFTFGLTVV QVYDNGQVFP SSILDITDEE LVSHFVSAVS TIASISLAIG YPTLPSVGHT LI NNYKDLL AVAIAASYHY PEIEDLVDRI ENPEKYAAAA PAATSAASGD AAPAEEAAAE EEEESDDDMG FGLFD

UniProtKB: Large ribosomal subunit protein uL10

Macromolecule #50: CAT-tailed nascent peptide

• Macromolecule: Name: CAT-tailed nascent peptide / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 1.464797 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #51: Large ribosomal subunit protein uL1A

• Macromolecule: Name: Large ribosomal subunit protein uL1A / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.524799 KDa

Sequence

String:

MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDD LKKLNKNKKL IKKLSKKYNA FIASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYGKVT DVRSTIKFQL K KVLCLAVA VGNVEMEEDV LVNQILMSVN FFVSLLKKNW QNVGSLVVKS SMGPAFRLY

UniProtKB: Large ribosomal subunit protein uL1A

Macromolecule #52: Large ribosomal subunit protein eL38

• Macromolecule: Name: Large ribosomal subunit protein eL38 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.845561 KDa

Sequence

String:

MAREITDIKQ FLELTRRADV KTATVKINKK LNKAGKPFRQ TKFKVRGSSS LYTLVINDAG KAKKLIQSLP PTLKVNRL

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #53: Ribosome quality control complex subunit 1

• Macromolecule: Name: Ribosome quality control complex subunit 1 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 87.005234 KDa
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

MSSRALRRLQ DDNALLESLL SNSNANKMTS GKSTAGNIQK RENIFSMMNN VRDSDNSTDE GQMSEQDEEA AAAGERDTQS NGQPKRITL ASKSSRRKKN KKAKRKQKNH TAEAAKDKGS DDDDDDEEFD KIIQQFKKTD ILKYGKTKND DTNEEGFFTA S EPEEASSQ PWKSFLSLES DPGFTKFPIS CLRHSCKFFQ NDFKKLDPHT EFKLLFDDIS PESLEDIDSM TSTPVSPQQL KQ IQRLKRL IRNWGGKDHR LAPNGPGMHP QHLKFTKIRD DWIPTQRGEL SMKLLSSDDL LDWQLWERPL DWKDVIQNDV SQW QKFISF YKFEPLNSDL SKKSMMDFYL SVIVHPDHEA LINLISSKFP YHVPGLLQVA LIFIRQGDRS NTNGLLQRAL FVFD RALKA NIIFDSLNCQ LPYIYFFNRQ FYLAIFRYIQ SLAQRGVIGT ASEWTKVLWS LSPLEDPLGC RYFLDHYFLL NNDYQ YIIE LSNSPLMNCY KQWNTLGFSL AVVLSFLRIN EMSSARNALL KAFKHHPLQL SELFKEKLLG DHALTKDLSI DGHSAE NLE LKAYMARFPL LWNRNEEVTF LHDEMSSILQ DYHRGNVTID SNDGQDHNNI NNLQSPFFIA GIPINLLRFA ILSEESS VM AAIPSFIWSD NEVYEFDVLP PMPTSKESIE VVENIKTFIN EKDLAVLQAE RMQDEDLLNQ IRQISLQQYI HENEESNE N EGTSGSPGDY KDDDDKDYKD DDDKDYKDDD DK

UniProtKB: Ribosome quality control complex subunit 1

Macromolecule #54: Polyubiquitin-C

• Macromolecule: Name: Polyubiquitin-C / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.576831 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

UniProtKB: Polyubiquitin-C

Macromolecule #55: 60S ribosomal protein L16-A

• Macromolecule: Name: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.247227 KDa

Sequence

String:

MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY AKKRAFTKKV ASANATAAES DVAKQLAALG Y

UniProtKB: Large ribosomal subunit protein uL13A

Macromolecule #56: 60S ribosomal protein L25

• Macromolecule: Name: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.787612 KDa

Sequence

String:

MAPSAKATAA KKAVVKGTNG KKALKVRTSA TFRLPKTLKL ARAPKYASKA VPHYNRLDSY KVIEQPITSE TAMKKVEDGN ILVFQVSMK ANKYQIKKAV KELYEVDVLK VNTLVRPNGT KKAYVRLTAD YDALDIANRI GYI

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #29: 25S rRNA

• Macromolecule: Name: 25S rRNA / type: rna / ID: 29 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 1.0970864999999999 MDa

Sequence

String:

GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU UGGGGCCGUU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA GU GCGGUUC UUUGUAAAGU GCCUUCGAAG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGUG UUUUGUGCCC UCUGCUCCUU GUGGGUAGGG GAAUC UCGC AUUUCACUGG CCAGCAUCAG UUUUGGUGGC AGGAUAAAUC CAUAGGAAUG UAGCUUGCCU CGGUAAGUAU UAUAGC CUG UGGGAAUACU GCCAGCUGGG ACUGAGGACU GCGACGUAAG UCAAGGAUGC UGGCAUAAUG GUUAUAUGCC GCCCGUC UU GAAACACGGA CCAAGGAGUC UAACGUCUAU GCGAGUGUUU GGGUGUAAAA CCCAUACGCG UAAUGAAAGU GAACGUAG G UUGGGGCCUC GCAAGAGGUG CACAAUCGAC CGAUCCUGAU GUCUUCGGAU GGAUUUGAGU AAGAGCAUAG CUGUUGGGA CCCGAAAGAU GGUGAACUAU GCCUGAAUAG GGUGAAGCCA GAGGAAACUC UGGUGGAGGC UCGUAGCGGU UCUGACGUGC AAAUCGAUC GUCGAAUUUG GGUAUAGGGG CGAAAGACUA AUCGAACCAU CUAGUAGCUG GUUCCUGCCG AAGUUUCCCU C AGGAUAGC AGAAGCUCGU AUCAGUUUUA UGAGGUAAAG CGAAUGAUUA GAGGUUCCGG GGUCGAAAUG ACCUUGACCU AU UCUCAAA CUUUAAAUAU GUAAGAAGUC CUUGUUACUU AAUUGAACGU GGACAUUUGA AUGAAGAGCU UUUAGUGGGC CAU UUUUGG UAAGCAGAAC UGGCGAUGCG GGAUGAACCG AACGUAGAGU UAAGGUGCCG GAAUACACGC UCAUCAGACA CCAC AAAAG GUGUUAGUUC AUCUAGACAG CCGGACGGUG GCCAUGGAAG UCGGAAUCCG CUAAGGAGUG UGUAACAACU CACCG GCCG AAUGAACUAG CCCUGAAAAU GGAUGGCGCU CAAGCGUGUU ACCUAUACUC UACCGUCAGG GUUGAUAUGA UGCCCU GAC GAGUAGGCAG GCGUGGAGGU CAGUGACGAA GCCUAGACCG UAAGGUCGGG UCGAACGGCC UCUAGUGCAG AUCUUGG UG GUAGUAGCAA AUAUUCAAAU GAGAACUUUG AAGACUGAAG UGGGGAAAGG UUCCACGUCA ACAGCAGUUG GACGUGGG U UAGUCGAUCC UAAGAGAUGG GGAAGCUCCG UUUCAAAGGC CUGAUUUUAU GCAGGCCACC AUCGAAAGGG AAUCCGGUU AAGAUUCCGG AACCUGGAUA UGGAUUCUUC ACGGUAACGU AACUGAAUGU GGAGACGUCG GCGCGAGCCC UGGGAGGAGU UAUCUUUUC UUCUUAACAG CUUAUCACCC CGGAAUUGGU UUAUCCGGAG AUGGGGUCUU AUGGCUGGAA GAGGCCAGCA C CUUUGCUG GCUCCGGUGC GCUUGUGACG GCCCGUGAAA AUCCACAGGA AGGAAUAGUU UUCAUGCCAG GUCGUACUGA UA ACCGCAG CAGGUCUCCA AGGUGAACAG CCUCUAGUUG AUAGAAUAAU GUAGAUAAGG GAAGUCGGCA AAAUAGAUCC GUA ACUUCG GGAUAAGGAU UGGCUCUAAG GGUCGGGUUA GUGAGGGCCU UGGUCAGACG CAGCGGGCGU GCUUGUGGAC UGCU UGGUG GGGCUUGCUC UGCUAGGCGG ACUACUUGCG UGCCUUGUUG UAGACGGCCU UGGUAGGUCU CUUGUAGACC GUCGC UUGC UACAAUUACG AUCAACUUAG AACUGGUACG GACAAGGGGA AUCUGACUGU CUAAUUAAAA CAUAGCAUUG CGAUGG UCA GAAAGUGAUG UUGACGCAAU GUGAUUUCUG CCCAGUGCUC UGAAUGUCAA AGUGAAGAAA UUCAACCAAG CGCGGGU AA ACGGCGGGAG UUAACUAUAC UCUCUUAAGG UAGCCAAAUG CCUCGUCAUC UAAUUAGUGA CGCGCAUGAA UGGAUUAA C GAGAUUCCCA CUGUCCCUAU CUACUAUCUA GCGAAACCAC AGCCAAGGGA ACGGGCUUGG CAGAAUCAGC GGGGAAAGA AGACCCUGUU GAGCUUGACU CUAGUUUGAC AUUGUGAAGA GACAUAGAGG GUGUAGAAUA AGUGGGAGCU UCGGCGCCAG UGAAAUACC ACUACCUUUA UAGUUUCUUU ACUUAUUCAA UGAAGCGGAG CUGGAAUUCA UUUUCCACGU UCUAGCAUUC A AGGUCCCA UUCGGGGCUG AUCCGGGUUG AAGACAUUGU CAGGUGGGGA GUUUGGCUGG GGCGGCACAU CUGUUAAACG AU AACGCAG AUGUCCUAAG GGGGGCUCAU GGAGAACAGA AAUCUCCAGU AGAACAAAAG GGUAAAAGCC CCCUUGAUUU UGA UUUUCA GUGUGAAUAC AAACCAUGAA AGUGUGGCCU AUCGAUCCUU UAGUCCCUCG GAAUUUGAGG CUAGAGGUGC CAGA AAAGU UACCACAGGG AUAACUGGCU UGUGGCAGUC AAGCGUUCAU AGCGACAUUG CUUUUUGAUU CUUCGAUGUC GGCUC UUCC UAUCAUACCG AAGCAGAAUU CGGUAAGCGU UGGAUUGUUC ACCCACUAAU AGGGAACGUG AGCUGGGUUU AGACCG UCG UGAGACAGGU UAGUUUUACC CUACUGAUGA AUGUUACCGC AAUAGUAAUU GAACUUAGUA CGAGAGGAAC AGUUCAU UC GGAUAAUUGG UUUUUGCGGC UGUCUGAUCA GGCAUUGCCG CGAAGCUACC AUCCGCUGGA UUAUGGCUGA ACGCCUCU A AGUCAGAAUC CAUGCUAGAA CGCGGUGAUU UCUUUGCUCC ACACAAUAUA GAUGGAUACG AAUAAGGCGU CCUUGUGGC GUCGCUGAAC CAUAGCAGGC UAGCAACGGU GCACUUGGCG GAAAGGCCUU GGGUGCUUGC UGGCGAAUUG CAAUGUCAUU UUGCGUGGG GAUAAAUCAU UUGUAUACGA CUUAGAUGUA CAACGGGGUA UUGUAAGCAG UAGAGUAGCC UUGUUGUUAC G AUCUGCUG AGAUUAAGCC UUUGUUGUCU GAUUUGU

GENBANK: GENBANK: U53879.1

Macromolecule #30: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 30 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 38.951105 KDa

Sequence

String:

GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

GENBANK: GENBANK: CP008434.1

Macromolecule #31: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 31 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 50.682922 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: KY109257.1

Macromolecule #47: Ala tRNA

• Macromolecule: Name: Ala tRNA / type: rna / ID: 47 / Number of copies: 2
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.502477 KDa

Sequence

String:

GGGCGUGUGG CGUAGUCGGU AGCGCGCUCC CUUAGCAUGG GAGAGGUCUC CGGUUCGAUU CCGGACUCGU CCGCCA

Macromolecule #57: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 57 / Number of copies: 9 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Macromolecule #58: ADENOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 58 / Number of copies: 1 / Formula: ADP
• Molecular weight: Theoretical: 427.201 Da

Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Macromolecule #59: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 59 / Number of copies: 7 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #60: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 60 / Number of copies: 12 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #61: SPERMIDINE

• Macromolecule: Name: SPERMIDINE / type: ligand / ID: 61 / Number of copies: 1 / Formula: SPD
• Molecular weight: Theoretical: 145.246 Da

Chemical component information

ChemComp-SPD:
SPERMIDINE

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 36.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.8 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: NONE
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.16 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 28262
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD