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- EMDB-70070: CryoEM structure of mu-opioid receptor - Gi protein complex bound... -

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Open data


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Basic information

Entry
Database: EMDB / ID: EMD-70070
TitleCryoEM structure of mu-opioid receptor - Gi protein complex bound to FNZ- local map
Map data
Sample
  • Complex: MOR-Gi heterotrimer complex
KeywordsComplex / Receptor / Agonist / MOR / MEMBRANE PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.6 Å
AuthorsRobertson MJ / Skiniotis G
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: CryoEM structure of mu-opioid receptor - Gi protein complex bound to fluornitrazene (FNZ)
Authors: Gomez JL / Ventriglia EN / Frangos ZJ / Sulima A / Robertson MJ / Sacco M / Budinich R / Giosan I / Solis O / Tischer AE / Bossert JM / Caldwell KE / Bonbrest H / Essmann A / Garcon-Poca MZ ...Authors: Gomez JL / Ventriglia EN / Frangos ZJ / Sulima A / Robertson MJ / Sacco M / Budinich R / Giosan I / Solis O / Tischer AE / Bossert JM / Caldwell KE / Bonbrest H / Essmann A / Garcon-Poca MZ / Choi S / Noya MR / Glatfelter G / Robinson M / Mullarkey AA / Brademan DR / Enten G / Dunne W / Schoenborn I / Paule J / Rais R / Holt DP / Dannals RF / Tanda G / Newman AH / Shi L / Huttenhain R / Ferre S / Kiyatkin E / Bonaventura J / Shaham Y / Zachariou V / Baumann MH / Skiniotis G / Rice KC / Michaelides M
History
DepositionApr 5, 2025-
Header (metadata) releaseApr 8, 2026-
Map releaseApr 8, 2026-
UpdateApr 8, 2026-
Current statusApr 8, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70070.png

Downloads & links

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Map

FileDownload / File: emd_70070.map.gz / Format: CCP4 / Size: 209.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.86 Å/pix.
x 380 pix.
= 326.8 Å		0.86 Å/pix.
x 380 pix.
= 326.8 Å		0.86 Å/pix.
x 380 pix.
= 326.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.86 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.315
Minimum - Maximum-1.5674887 - 2.438668
Average (Standard dev.)-0.00076987845 (±0.032286048)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions380380380
Spacing380380380
CellA=B=C: 326.80002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_70070_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_70070_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : MOR-Gi heterotrimer complex

EntireName: MOR-Gi heterotrimer complex
Components
  • Complex: MOR-Gi heterotrimer complex

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Supramolecule #1: MOR-Gi heterotrimer complex

SupramoleculeName: MOR-Gi heterotrimer complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 56.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.6 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 667110
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

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