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- EMDB-6846: Volta phase contrast cryotomogram of a cryosectioned prometaphase... -
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Open data
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Basic information
Entry | Database: EMDB / ID: 6846 |
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Title | Volta phase contrast cryotomogram of a cryosectioned prometaphase S. pombe cell, strain nda3-KM311 |
![]() | Volta phase contrast cryotomogram of a cryosectioned prometaphase S. pombe cell, strain nda3-KM311 |
![]() | S. pombe cell, arrested in prometaphase: |
Source | ![]() ![]() ![]() |
Method | ![]() ![]() |
![]() | Cai S / Chen C / Tan Z / Huang Y / Shi J / Gan L |
![]() | Journal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2018 Title: Cryo-ET reveals the macromolecular reorganization of mitotic chromosomes in vivo. ![]() |
Date | Deposition: Nov 21, 2017 / Header (metadata) release: Oct 31, 2018 / Map release: Oct 31, 2018 / Last update: Nov 7, 2018 |
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Structure visualization
Movie |
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Supplemental images |
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Downloads & links
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Download
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Header (meta data in XML format) | ![]() ![]() ![]() |
Images | ![]() |
FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-6846 |
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Links
EMDB pages | ![]() ![]() |
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EM raw data | ![]() Data size: 35.1 Data #1: Tilt series of fission yeast cells and chromatin lysates in various cell cycle states [tilt series]) ![]() Data size: 95.4 Data #1: Tilt series, tomograms, and models of cryosections of mitotic budding yeast and reconstituted DASH rings around microtubules [tilt series]) |
-Related structure data
Similar-shape strucutres |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4.6 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire S. pombe cell, arrested in prometaphase
Entire | Name: S. pombe cell, arrested in prometaphase / Number of components: 1 |
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-Component #1: cellular-component, S. pombe cell, arrested in prometaphase
Cellular-component | Name: S. pombe cell, arrested in prometaphase / Recombinant expression: No |
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Source | Species: ![]() ![]() ![]() |
-Experimental details
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Sample preparation
Specimen | Specimen state: cell / Method: ![]() |
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Sample solution | Buffer solution: yeast-extract supplemented / pH: 7 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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![]() | Microscope: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Magnification: 18000.0 X (nominal), 30400.0 X (calibrated) / Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: FEI TITAN KRIOS AUTOGRID HOLDER |
Camera | Detector: FEI FALCON II (4k x 4k) |
-Image acquisition
Image acquisition | Number of digital images: 61 / Sampling size: 14 microns |
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Raw data | ![]() Data size: 35.1 Data #1: Tilt series of fission yeast cells and chromatin lysates in various cell cycle states [tilt series]) ![]() Data size: 95.4 Data #1: Tilt series, tomograms, and models of cryosections of mitotic budding yeast and reconstituted DASH rings around microtubules [tilt series]) |
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Image processing
![]() | Method: ![]() |
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3D reconstruction | Algorithm: BACK PROJECTION / Software: ![]() |