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Yorodumi- EMDB-6841: Complex structure of Pseudorabies virus glycoprotein B bound by a... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-6841 | |||||||||
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Title | Complex structure of Pseudorabies virus glycoprotein B bound by a neutralizing antibody | |||||||||
Map data | EM density map | |||||||||
Sample |
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Biological species | Suid herpesvirus 1 | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 35.0 Å | |||||||||
Authors | Yang F / Peng R / Gao GF / Shi Y | |||||||||
Citation | Journal: PLoS Pathog / Year: 2017 Title: Two classes of protective antibodies against Pseudorabies virus variant glycoprotein B: Implications for vaccine design. Authors: Xiangdong Li / Fanli Yang / Xule Hu / Feifei Tan / Jianxun Qi / Ruchao Peng / Min Wang / Yan Chai / Liying Hao / Junhua Deng / Chenyu Bai / Juan Wang / Hao Song / Shuguang Tan / Guangwen Lu ...Authors: Xiangdong Li / Fanli Yang / Xule Hu / Feifei Tan / Jianxun Qi / Ruchao Peng / Min Wang / Yan Chai / Liying Hao / Junhua Deng / Chenyu Bai / Juan Wang / Hao Song / Shuguang Tan / Guangwen Lu / George F Gao / Yi Shi / Kegong Tian / Abstract: Pseudorabies virus (PRV) belongs to the Herpesviridae family, and is an important veterinary pathogen. Highly pathogenic PRV variants have caused severe epidemics in China since 2011, causing huge ...Pseudorabies virus (PRV) belongs to the Herpesviridae family, and is an important veterinary pathogen. Highly pathogenic PRV variants have caused severe epidemics in China since 2011, causing huge economic losses. To tackle the epidemics, we identified a panel of mouse monoclonal antibodies (mAbs) against PRV glycoprotein B (gB) that effectively block PRV infection. Among these 15 mAbs, fourteen of them block PRV entry in a complement-dependent manner. The remaining one, 1H1 mAb, however can directly neutralize the virus independent of complement and displays broad-spectrum neutralizing activities. We further determined the crystal structure of PRV gB and mapped the epitopes of these antibodies on the structure. Interestingly, all the complement-dependent neutralizing antibodies bind gB at the crown region (domain IV). In contrast, the epitope of 1H1 mAb is located at the bottom of domain I, which includes the fusion loops, indicating 1H1 mAb might neutralize the virus by interfering with the membrane fusion process. Our studies demonstrate that gB contains multiple B-cell epitopes in its crown and base regions and that antibodies targeting different epitopes block virus infection through different mechanisms. These findings would provide important clues for antiviral drug design and vaccine development. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_6841.map.gz | 97.1 MB | EMDB map data format | |
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Header (meta data) | emd-6841-v30.xml emd-6841.xml | 10.4 KB 10.4 KB | Display Display | EMDB header |
Images | emd_6841.png | 13.6 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-6841 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-6841 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_6841.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | EM density map | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.36 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Pseudorabies virus glycoprotein B in complex with Fab fragments f...
Entire | Name: Pseudorabies virus glycoprotein B in complex with Fab fragments from a neutralizing antibody |
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Components |
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-Supramolecule #1: Pseudorabies virus glycoprotein B in complex with Fab fragments f...
Supramolecule | Name: Pseudorabies virus glycoprotein B in complex with Fab fragments from a neutralizing antibody type: complex / ID: 1 / Parent: 0 Details: The Fab fragment was generated by proteolytic cleavage of IgG antibody. The Pseudorabies virus glycoprotein B was expressed using the baculovirus expression system. The two proteins were ...Details: The Fab fragment was generated by proteolytic cleavage of IgG antibody. The Pseudorabies virus glycoprotein B was expressed using the baculovirus expression system. The two proteins were purified separately and mixed to constitute complex samples in solution. |
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Source (natural) | Organism: Suid herpesvirus 1 |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) / Recombinant cell: Sf9 |
Molecular weight | Experimental: 300 KDa |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.02 mg/mL |
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Buffer | pH: 8 / Details: 20 mM Tris-HCl, pH 8.0 150 mM NaCl |
Staining | Type: NEGATIVE / Material: Uranyl acetate Details: The specimen was stained by 1% Uranyl acetate for 1 min and air-dried before data acquisition. |
Grid | Model: Homemade / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 10.0 nm / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR |
-Electron microscopy
Microscope | FEI TECNAI F20 |
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Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: FEI EAGLE (4k x 4k) / Average electron dose: 30.0 e/Å2 |
Experimental equipment | Model: Tecnai F20 / Image courtesy: FEI Company |
-Image processing
CTF correction | Software - Name: ctfit |
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Startup model | Type of model: OTHER Details: Newly determined crystal structure of Pseudorabies virus glycoprotein B. |
Initial angle assignment | Type: RANDOM ASSIGNMENT / Software - Name: RELION (ver. 2.0) |
Final 3D classification | Software - Name: RELION (ver. 2.0) |
Final angle assignment | Type: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2.0) |
Final reconstruction | Number classes used: 1 / Applied symmetry - Point group: C3 (3 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 35.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: RELION (ver. 2.0) / Number images used: 3000 |
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: RIGID BODY FIT |
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