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- EMDB-6816: NuA4 TEEAA sub-complex -

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Basic information

Entry
Database: EMDB / ID: EMD-6816
TitleNuA4 TEEAA sub-complex
Map data
Sample
  • Complex: NuA4 TEEAA sub-complex
    • Protein or peptide: Transcription-associated protein 1
    • Protein or peptide: Chromatin modification-related protein EAF1
    • Protein or peptide: Eaf1-disorder domain
    • Protein or peptide: Chromatin modification-related protein EAF5
    • Protein or peptide: Transcription-associated protein 1
    • Protein or peptide: Actin-related protein 4
    • Protein or peptide: Actin
    • Protein or peptide: Chromatin modification-related protein EAF1
Function / homology
Function and homology information


RHOB GTPase cycle / RHOA GTPase cycle / cellular bud neck contractile ring / mitotic actomyosin contractile ring contraction / nucleosome disassembly/reassembly complex / ascospore wall assembly / vacuole inheritance / actin cortical patch / Swr1 complex / SLIK (SAGA-like) complex ...RHOB GTPase cycle / RHOA GTPase cycle / cellular bud neck contractile ring / mitotic actomyosin contractile ring contraction / nucleosome disassembly/reassembly complex / ascospore wall assembly / vacuole inheritance / actin cortical patch / Swr1 complex / SLIK (SAGA-like) complex / kinetochore assembly / DNA replication-dependent chromatin assembly / Ino80 complex / SWI/SNF complex / nucleosome disassembly / SAGA complex / establishment of cell polarity / NuA4 histone acetyltransferase complex / actin filament bundle / protein secretion / Ub-specific processing proteases / actin filament / positive regulation of transcription elongation by RNA polymerase II / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / structural constituent of cytoskeleton / endocytosis / chromatin organization / histone binding / protein-containing complex assembly / chromatin remodeling / DNA repair / DNA-templated transcription / chromatin binding / regulation of DNA-templated transcription / chromatin / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / ATP hydrolysis activity / ATP binding / identical protein binding / nucleus / cytosol
Similarity search - Function
Chromatin modification-related protein EAF5 / Tra1, HEAT repeat ring region / Tra1, HEAT repeat central region / Tra1 HEAT repeat central region / Tra1 HEAT repeat ring region / Myb-like domain profile. / domain in helicases and associated with SANT domains / Myb-like DNA-binding domain / HSA domain / Helicase/SANT-associated domain ...Chromatin modification-related protein EAF5 / Tra1, HEAT repeat ring region / Tra1, HEAT repeat central region / Tra1 HEAT repeat central region / Tra1 HEAT repeat ring region / Myb-like domain profile. / domain in helicases and associated with SANT domains / Myb-like DNA-binding domain / HSA domain / Helicase/SANT-associated domain / HSA domain profile. / : / PIK-related kinase, FAT / FAT domain / FAT domain profile. / FATC domain profile. / FATC domain / PIK-related kinase / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / SANT/Myb domain / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Actins signature 1. / Actin, conserved site / Actins signature 2. / Actin/actin-like conserved site / Actins and actin-related proteins signature. / Actin / Actin family / Actin / Homeobox-like domain superfamily / ATPase, nucleotide binding domain / Armadillo-type fold / Protein kinase-like domain superfamily
Similarity search - Domain/homology
Transcription-associated protein 1 / Chromatin modification-related protein EAF5 / Actin / Actin-related protein 4 / Chromatin modification-related protein EAF1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) / Baker's yeast (brewer's yeast) / Saccharomyces cerevisiae (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / negative staining / Resolution: 4.7 Å
AuthorsWang X / Cai G
Funding support China, 3 items
OrganizationGrant numberCountry
National Basic Research Program2014CB910700 China
the National Natural Science Foundation of China31170694 China
the National Natural Science Foundation of China31570726 China
CitationJournal: Nat Commun / Year: 2018
Title: Architecture of the Saccharomyces cerevisiae NuA4/TIP60 complex.
Authors: Xuejuan Wang / Salar Ahmad / Zhihui Zhang / Jacques Côté / Gang Cai /
Abstract: The NuA4/TIP60 acetyltransferase complex is required for gene regulation, DNA repair and cell cycle progression. The limited structural information impeded understanding of NuA4/TIP60 assembly and ...The NuA4/TIP60 acetyltransferase complex is required for gene regulation, DNA repair and cell cycle progression. The limited structural information impeded understanding of NuA4/TIP60 assembly and regulatory mechanism. Here, we report the 4.7 Å cryo-electron microscopy (cryo-EM) structure of a NuA4/TIP60 TEEAA assembly (Tra1, Eaf1, Eaf5, actin and Arp4) and the 7.6 Å cryo-EM structure of a TEEAA-piccolo assembly (Esa1, Epl1, Yng2 and Eaf6). The Tra1 and Eaf1 constitute the assembly scaffold. The Eaf1 SANT domain tightly binds to the LBE and FATC domains of Tra1 by ionic interactions. The actin/Arp4 peripherally associates with Eaf1 HSA domain. The Eaf5/7/3 (TINTIN) and piccolo modules largely pack against the FAT and HEAT repeats of Tra1 and their association depends on Eaf1 N-terminal and HSA regions, respectively. These structures elucidate the detailed architecture and molecular interactions between NuA4 subunits and offer exciting insights into the scaffolding and regulatory mechanisms of Tra1 pseudokinase.
History
DepositionAug 18, 2017-
Header (metadata) releaseApr 18, 2018-
Map releaseApr 18, 2018-
UpdateNov 6, 2019-
Current statusNov 6, 2019Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0225
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.0225
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5y81
  • Surface level: 0.0225
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_6816.png

Downloads & links

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Map

FileDownload / File: emd_6816.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.3 Å/pix.
x 288 pix.
= 374.4 Å		1.3 Å/pix.
x 288 pix.
= 374.4 Å		1.3 Å/pix.
x 288 pix.
= 374.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.3 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0225 / Movie #1: 0.0225
Minimum - Maximum-0.062639356 - 0.13194954
Average (Standard dev.)0.00026157088 (±0.005168174)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions288288288
Spacing288288288
CellA=B=C: 374.4 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.31.31.3
M x/y/z288288288
origin x/y/z0.0000.0000.000
length x/y/z374.400374.400374.400
α/β/γ90.00090.00090.000
start NX/NY/NZ-128-128-128
NX/NY/NZ256256256
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS288288288
D min/max/mean-0.0630.1320.000

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Supplemental data

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Sample components

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Entire : NuA4 TEEAA sub-complex

EntireName: NuA4 TEEAA sub-complex
Components
  • Complex: NuA4 TEEAA sub-complex
    • Protein or peptide: Transcription-associated protein 1
    • Protein or peptide: Chromatin modification-related protein EAF1
    • Protein or peptide: Eaf1-disorder domain
    • Protein or peptide: Chromatin modification-related protein EAF5
    • Protein or peptide: Transcription-associated protein 1
    • Protein or peptide: Actin-related protein 4
    • Protein or peptide: Actin
    • Protein or peptide: Chromatin modification-related protein EAF1

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Supramolecule #1: NuA4 TEEAA sub-complex

SupramoleculeName: NuA4 TEEAA sub-complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c

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Macromolecule #1: Transcription-associated protein 1

MacromoleculeName: Transcription-associated protein 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 130.470539 KDa
SequenceString: YNAWYQSINI LESIQSNTSI DNTKIIEANE DALLELYVNL QEEDMFYGLW RRRAKYTETN IGLSYEQIGL WDKAQQLYEV AQVKARSGA LPYSQSEYAL WEDNWIQCAE KLQHWDVLTE LAKHEGFTDL LLECGWRVAD WNSDRDALEQ SVKSVMDVPT P RRQMFKTF ...String:
YNAWYQSINI LESIQSNTSI DNTKIIEANE DALLELYVNL QEEDMFYGLW RRRAKYTETN IGLSYEQIGL WDKAQQLYEV AQVKARSGA LPYSQSEYAL WEDNWIQCAE KLQHWDVLTE LAKHEGFTDL LLECGWRVAD WNSDRDALEQ SVKSVMDVPT P RRQMFKTF LALQNFAESR KGDQEVRKLC DEGIQLSLIK WVSLPIRYTP AHKWLLHGFQ QYMEFLEATQ IYANLHTTTV QN LDSKAQE IKRILQAWRD RLPNTWDDVN MWNDLVTWRQ HAFQVINNAY LPLIPALQQS NSNSNINTHA YRGYHEIAWV INR FAHVAR KHNMPDVCIS QLARIYTLPN IEIQEAFLKL REQAKCHYQN MNELTTGLDV ISNTNLVYFG TVQKAEFFTL KGMF LSKLR AYEEANQAFA TAVQIDLNLA KAWAQWGFFN DRRLSEEPNN ISFASNAISC YLQAAGLYKN SKIRELLCRI LWLIS IDDA SGMLTNAFDS FRGEIPVWYW ITFIPQLLTS LSHKEANMVR HILIRIAKSY PQALHFQLRT TKEDFAVIQR QTMAVM GDK PDTNDRNGRR QPWEYLQELN NILKTAYPLL ALSLESLVAQ INDRFKSTTD EDLFRLINVL LIDGTLNYNR LPFPRKN PK LPENTEKNLV KFSTTLLAPY IRPKFNADFI DNKPDYETYI KRLRYWRRRL ENKLDRASKK ENLEVLCPHL SNFHHQKF E DIEIPGQYLL NKDNNVHFIK IARFLPTVDF VRGTHSSYRR LMIRGHDGSV HSFAVQYPAV RHSRREERMF QLYRLFNKS LSKNVETRRR SIQFNLPIAI PLSPQVRIMN DSVSFTTLHE IHNEFCKKKG FDPDDIQDFM ADKLNAAHDD ALPAPDMTIL KVEIFNSIQ TMFVPSNVLK DHFTSLFTQF EDFWLFRKQF ASQYSSFVFM SYMMMINNRT PHKIHVDKTS GNVFTLEMLP S RFPYERVK PLLKNHDLSL PPDSPIFHNN EPVPFRLTPN IQSLIGDSAL EGIFAVNLFT ISRALIEPDN ELNTYLALFI RD EIISWFS NLHRPIIENP QLREMVQTNV DLIIRKVAQL GHLNSTPTVT TQFILDCIGS AVSPRNLART DVNFMPWF

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Macromolecule #2: Chromatin modification-related protein EAF1

MacromoleculeName: Chromatin modification-related protein EAF1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 39.431434 KDa
SequenceString: PTIWLSEDDQ ELVKNINTYG YNWELISAHM THRLTYSYLS NIERRTPWQC FERFVQLNER FNFSDLKGPR AHSAQQWLIE AHKFQQRQN RRISPLGVNT ESIQRGHRRL RWASMFEAIR KCMKKRENTP RPNPTQPRKP LDCKNMKVPT PAEMSLLKAQ R DEALRRDI ...String:
PTIWLSEDDQ ELVKNINTYG YNWELISAHM THRLTYSYLS NIERRTPWQC FERFVQLNER FNFSDLKGPR AHSAQQWLIE AHKFQQRQN RRISPLGVNT ESIQRGHRRL RWASMFEAIR KCMKKRENTP RPNPTQPRKP LDCKNMKVPT PAEMSLLKAQ R DEALRRDI QLRRTVKNRL QQRQQQSQQA HSSRAQSPIP SNGKSSSNLA RNGQASAPRP NQKQYTEQDI IESYSRKLLE QK PDIGPEM ALKAAKNYYR TLREQQQQLK QHQIQQQRQQ LQEESSHVQQ LQQLQPGSQA PPPKSSPSQS SLSNISNINS APR IKSPTP QEILQRFQKQ

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Macromolecule #3: Eaf1-disorder domain

MacromoleculeName: Eaf1-disorder domain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 42.570492 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)

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Macromolecule #4: Chromatin modification-related protein EAF5

MacromoleculeName: Chromatin modification-related protein EAF5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 31.689264 KDa
SequenceString: MDKEVSELVV LQLIHTLISN KNEELVRNGG GINMIGNNLR ISLVKLTNEI QNNLLINELT NLRRQSNVAN GNRKLGINDI LTIVKNLFP EYRTTLNDGQ LSLHGLEMHD IEKLLDEKYD RFKKTQVEQI RMMEDEILKN GIKTGASQLQ PHANAGKSGS A GTSATITT ...String:
MDKEVSELVV LQLIHTLISN KNEELVRNGG GINMIGNNLR ISLVKLTNEI QNNLLINELT NLRRQSNVAN GNRKLGINDI LTIVKNLFP EYRTTLNDGQ LSLHGLEMHD IEKLLDEKYD RFKKTQVEQI RMMEDEILKN GIKTGASQLQ PHANAGKSGS A GTSATITT TTPHMAHSMD PKREKLLKLY RDTVLNKLES KTGNFQKLFK SPDGSIIKNE INYEDIKNET PGSVHELQLI LQ KSITDGV MRKVIGTDDW KLARQVQFEL DDTVQFMRRA LE

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Macromolecule #5: Transcription-associated protein 1

MacromoleculeName: Transcription-associated protein 1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 303.020531 KDa
SequenceString: MSLTEQIEQF ASRFRDDDAT LQSRYSTLSE LYDIMELLNS PEDYHFFLQA VIPLLLNQLK EVPISYDAHS PEQKLRNSML DIFNRCLMN QTFQPYAMEV LEFLLSVLPK ENEENGILCM KVLTTLFKSF KSILQDKLDS FIRIIIQIYK NTPNLINQTF Y EAGKAEQG ...String:
MSLTEQIEQF ASRFRDDDAT LQSRYSTLSE LYDIMELLNS PEDYHFFLQA VIPLLLNQLK EVPISYDAHS PEQKLRNSML DIFNRCLMN QTFQPYAMEV LEFLLSVLPK ENEENGILCM KVLTTLFKSF KSILQDKLDS FIRIIIQIYK NTPNLINQTF Y EAGKAEQG DLDSPKEPQA DELLDEFSKN DEEKDFPSKQ SSTEPRFENS TSSNGLRSSM FSFKILSECP ITMVTLYSSY KQ LTSTSLP EFTPLIMNLL NIQIKQQQEA REQAESRGEH FTSISTEIIN RPAYCDFILA QIKATSFLAY VFIRGYAPEF LQD YVNFVP DLIIRLLQDC PSELSSARKE LLHATRHILS TNYKKLFLPK LDYLFDERIL IGNGFTMHET LRPLAYSTVA DFIH NIRSE LQLSEIEKTI KIYTGYLLDE SLALTVQIMS AKLLLNLVER ILKLGKENPQ EAPRAKKLLM IIIDSYMNRF KTLNR QYDT IMKYYGRYET HKKEKAEKLK NSIQDNDKES EEFMRKVLEP SDDDHLMPQP KKEDINDSPD VEMTESDKVV KNDVEM FDI KNYAPILLLP TPTNDPIKDA FYLYRTLMSF LKTIIHDLKV FNPPPNEYTV ANPKLWASVS RVFSYEEVIV FKDLFHE CI IGLKFFKDHN EKLSPETTKK HFDISMPSLP VSATKDAREL MDYLAFMFMQ MDNATFNEII EQELPFVYER MLEDSGLL H VAQSFLTSEI TSPNFAGILL RFLKGKLKDL GNVDFNTSNV LIRLFKLSFM SVNLFPNINE VVLLPHLNDL ILNSLKYST TAEEPLVYFY LIRTLFRSIG GGRFENLYRS IKPILQVLLQ SLNQMILTAR LPHERELYVE LCITVPVRLS VLAPYLPFLM KPLVFALQQ YPDLVSQGLR TLELCIDNLT AEYFDPIIEP VIDDVSKALF NLLQPQPFNH AISHNVVRIL GKLGGRNRQF L KPPTDLTE KTELDIDAIA DFKINGMPED VPLSVTPGIQ SALNILQSYK SDIHYRKSAY KYLTCVLLLM TKSSAEFPTN YT ELLKTAV NSIKLERIGI EKNFDLEPTV NKRDYSNQEN LFLRLLESVF YATSIKELKD DAMDLLNNLL DHFCLLQVNT TLL NKRNYN GTFNIDLKNP NFMLDSSLIL DAIPFALSYY IPEVREVGVL AYKRIYEKSC LIYGEELALS HSFIPELAKQ FIHL CYDET YYNKRGGVLG IKVLIDNVKS SSVFLKKYQY NLANGLLFVL KDTQSEAPSA ITDSAEKLLI DLLSITFADV KEEDL GNKV LENTLTDIVC ELSNANPKVR NACQKSLHTI SNLTGIPIVK LMDHSKQFLL SPIFAKPLRA LPFTMQIGNV DAITFC LSL PNTFLTFNEE LFRLLQESIV LADAEDESLS TNIQKTTEYS TSEQLVQLRI ACIKLLAIAL KNEEFATAQQ GNIRIRI LA VFFKTMLKTS PEIINTTYEA LKGSLAENSK LPKELLQNGL KPLLMNLSDH QKLTVPGLDA LSKLLELLIA YFKVEIGR K LLDHLTAWCR VEVLDTLFGQ DLAEQMPTKI IVSIINIFHL LPPQADMFLN DLLLKVMLLE RKLRLQLDSP FRTPLARYL NRFHNPVTEY FKKNMTLRQL VLFMCNIVQR PEAKELAEDF EKELDNFYDF YISNIPKNQV RVVSFFTNMV DLFNTMVITN GDEWLKKKG NMILKLKDML NLTLKTIKEN SFYIDHLQLN QSIAKFQALY LRFTELSERD QNPLLLDFID FSFSNGIKAS Y SLKKFIFH NIIASSNKEK QNNFINDATL FVLSDKCLDA RIFVLKNVIN STLIYEVATS GSLKSYLVED KKPKWLELLH NK IWKNSNA ILAYDVLDHH DLFRFELLQL SAIFIKADPE IIAEIKKDII KFCWNFIKLE DTLIKQSAYL VTSYFISKFD FPI KVVTQV FVALLRSSHV EARYLVKQSL DVLTPVLHER MNAAGTPDTW INWVKRVMVE NSSSQNNILY QFLISHPDLF FNSR DLFIS NIIHHMNKIT FMSNSNSDSH TLAIDLASLI LYWENKTLEI TNVNNTKTDS DGDVVMSDSK SDINPVEADT TAIIV DANN NSPISLHLRE ACTAFLIRYV CASNHRAIET ELGLRAINIL SELISDKHWT NVNVKLVYFE KFLIFQDLDS ENILYY CMN ALDVLYVFFK NKTKEWIMEN LPTIQNLLEK CIKSDHHDVQ EALQKVLQVI MKAIKAQGVS VIIEEESPGK TFIQMLT SV ITQDLQETSS VTAGVTLAWV LFMNFPDNIV PLLTPLMKTF SKLCKDHLSI SQPKDAMALE EARITTKLLE KVLYILSL K VSLLGDSRRP FLSTVALLID HSMDQNFLRK IVNMSRSWIF NTEIFPTVKE KAAILTKMLA FEIRGEPSLS KLFYEIVLK LFDQEHFNNT EITVRMEQPF LVGTRVEDIG IRKRFMTILD NSLERDIKER LYYVIRDQNW EFIADYPWLN QALQLLYGSF NREKELSLK NIYCLSPPSI LQEYLPENAE MVTEVNDLEL SNFVKGHIAS MQGLCRIISS DFIDSLIEIF YQDPKAIHRA W VTLFPQVY KSIPKNEKYG FVRSIITLLS KPYHTRQISS RTNVINMLLD SISKIESLEL PPHLVKYLA

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Macromolecule #6: Actin-related protein 4

MacromoleculeName: Actin-related protein 4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 54.991797 KDa
SequenceString: PMSNAALQVY GGDEVSAVVI DPGSYTTNIG YSGSDFPQSI LPSVYGKYTA DEGNKKIFSE QSIGIPRKDY ELKPIIENGL VIDWDTAQE QWQWALQNEL YLNSNSGIPA LLTEPVWNST ENRKKSLEVL LEGMQFEACY LAPTSTCVSF AAGRPNCLVV D IGHDTCSV ...String:
PMSNAALQVY GGDEVSAVVI DPGSYTTNIG YSGSDFPQSI LPSVYGKYTA DEGNKKIFSE QSIGIPRKDY ELKPIIENGL VIDWDTAQE QWQWALQNEL YLNSNSGIPA LLTEPVWNST ENRKKSLEVL LEGMQFEACY LAPTSTCVSF AAGRPNCLVV D IGHDTCSV SPIVDGMTLS KSTRRNFIAG KFINHLIKKA LEPKEIIPLF AIKQRKPEFI KKTFDYEVDK SLYDYANNRG FF QECKETL CHICPTKTLE ETKTELSSTA KRSIESPWNE EIVFDNETRY GFAEELFLPK EDDIPANWPR SNSGVVKTWR NDY VPLKRT KPSGVNKSDK KVTPTEEKEQ EAVSKSTSPA ANSADTPNET GKRPLEEEKP PKENNELIGL ADLVYSSIMS SDVD LRATL AHNVVLTGGT SSIPGLSDRL MTELNKILPS LKFRILTTGH TIERQYQSWL GGSILTSLGT FHQLWVGKKE YEEVG VERL LNDRFR

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Macromolecule #7: Actin

MacromoleculeName: Actin / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 41.735547 KDa
SequenceString: MDSEVAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGIMVGMGQK DSYVGDEAQS KRGILTLRYP IEHGIVTNWD DMEKIWHHT FYNELRVAPE EHPVLLTEAP MNPKSNREKM TQIMFETFNV PAFYVSIQAV LSLYSSGRTT GIVLDSGDGV T HVVPIYAG ...String:
MDSEVAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGIMVGMGQK DSYVGDEAQS KRGILTLRYP IEHGIVTNWD DMEKIWHHT FYNELRVAPE EHPVLLTEAP MNPKSNREKM TQIMFETFNV PAFYVSIQAV LSLYSSGRTT GIVLDSGDGV T HVVPIYAG FSLPHAILRI DLAGRDLTDY LMKILSERGY SFSTTAEREI VRDIKEKLCY VALDFEQEMQ TAAQSSSIEK SY ELPDGQV ITIGNERFRA PEALFHPSVL GLESAGIDQT TYNSIMKCDV DVRKELYGNI VMSGGTTMFP GIAERMQKEI TAL APSSMK VKIIAPPERK YSVWIGGSIL ASLTTFQQMW ISKQEYDESG PSIVHHKCF

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Macromolecule #8: Chromatin modification-related protein EAF1

MacromoleculeName: Chromatin modification-related protein EAF1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 4.882613 KDa
SequenceString:
THQNILLEEA KWMQADFKEG HKYKVAICTA MAQAIKDYWT Y

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Experimental details

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Structure determination

Methodnegative staining, cryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
StainingType: NONE / Material: Uranyl Formate
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 30.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 63197
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING

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