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- EMDB-66727: CryoEM structure of the G6PT dimer -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-66727
TitleCryoEM structure of the G6PT dimer
Map data
Sample
  • Complex: Glucose-6-phosphate translocase
    • Protein or peptide: Glucose-6-phosphate exchanger SLC37A4
  • Ligand: Lauryl Maltose Neopentyl Glycol
KeywordsGlucose-6-Phosphate Translocase / G6PT / SLC37A4 / TRANSPORT PROTEIN
Function / homology
Function and homology information


glucose-6-phosphate transmembrane transporter activity / Glycogen storage disease type Ib (SLC37A4) / glucose 6-phosphate:phosphate antiporter activity / glucose-6-phosphate transport / Gluconeogenesis / phosphate ion transmembrane transport / gluconeogenesis / glucose metabolic process / glucose homeostasis / endoplasmic reticulum membrane ...glucose-6-phosphate transmembrane transporter activity / Glycogen storage disease type Ib (SLC37A4) / glucose 6-phosphate:phosphate antiporter activity / glucose-6-phosphate transport / Gluconeogenesis / phosphate ion transmembrane transport / gluconeogenesis / glucose metabolic process / glucose homeostasis / endoplasmic reticulum membrane / endoplasmic reticulum / membrane
Similarity search - Function
Glycerate/sugar phosphate transporter, conserved site / : / glpT family of transporters signature. / Sugar phosphate transporter / Major facilitator superfamily / Major Facilitator Superfamily / Major facilitator superfamily domain / Major facilitator superfamily (MFS) profile. / MFS transporter superfamily
Similarity search - Domain/homology
Glucose-6-phosphate exchanger SLC37A4
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.27 Å
AuthorsZhang WQ / Jiao HZ / Hu HL / Guo X
Funding support1 items
OrganizationGrant numberCountry
Other government
CitationJournal: To Be Published
Title: Cryo-EM Structure of the Human Glucose-6-Phosphate Translocase in an outward-open conformation
Authors: Zhang WQ / Jiao HZ / Hu HL / Guo X
History
DepositionOct 25, 2025-
Header (metadata) releaseMar 18, 2026-
Map releaseMar 18, 2026-
UpdateMar 18, 2026-
Current statusMar 18, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_66727.png

Downloads & links

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Map

FileDownload / File: emd_66727.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.85 Å/pix.
x 320 pix.
= 272. Å		0.85 Å/pix.
x 320 pix.
= 272. Å		0.85 Å/pix.
x 320 pix.
= 272. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.85 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.45
Minimum - Maximum-1.4577543 - 2.0680475
Average (Standard dev.)-0.0000780321 (±0.03998444)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 272.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_66727_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_66727_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_66727_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Glucose-6-phosphate translocase

EntireName: Glucose-6-phosphate translocase
Components
  • Complex: Glucose-6-phosphate translocase
    • Protein or peptide: Glucose-6-phosphate exchanger SLC37A4
  • Ligand: Lauryl Maltose Neopentyl Glycol

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Supramolecule #1: Glucose-6-phosphate translocase

SupramoleculeName: Glucose-6-phosphate translocase / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Glucose-6-phosphate exchanger SLC37A4

MacromoleculeName: Glucose-6-phosphate exchanger SLC37A4 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 49.025609 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MDYKDDDDKA SRATMAAQGY GYYRTVIFSA MFGGYSLYYF NRKTFSFVMP SLVEEIPLDK DDLGFITSSQ SAAYAISKFV SGVLSDQMS ARWLFSSGLL LVGLVNIFFA WSSTVPVFAA LWFLNGLAQG LGWPPCGKVL RKWFEPSQFG TWWAILSTSM N LAGGLGPI ...String:
MDYKDDDDKA SRATMAAQGY GYYRTVIFSA MFGGYSLYYF NRKTFSFVMP SLVEEIPLDK DDLGFITSSQ SAAYAISKFV SGVLSDQMS ARWLFSSGLL LVGLVNIFFA WSSTVPVFAA LWFLNGLAQG LGWPPCGKVL RKWFEPSQFG TWWAILSTSM N LAGGLGPI LATILAQSYS WRSTLALSGA LCVVVSFLCL LLIHNEPADV GLRNLDPMPS EGKKGSLKEE STLQELLLSP YL WVLSTGY LVVFGVKTCC TDWGQFFLIQ EKGQSALVGS SYMSALEVGG LVGSIAAGYL SDRAMAKAGL SNYGNPRHGL LLF MMAGMT VSMYLFRVTV TSDSPKLWIL VLGAVFGFSS YGPIALFGVI ANESAPPNLC GTSHAIVGLM ANVGGFLAGL PFST IAKHY SWSTAFWVAE VICAASTAAF FLLRNIRTKM GRVSKKAESN SLEVLFQ

UniProtKB: Glucose-6-phosphate exchanger SLC37A4

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Macromolecule #2: Lauryl Maltose Neopentyl Glycol

MacromoleculeName: Lauryl Maltose Neopentyl Glycol / type: ligand / ID: 2 / Number of copies: 2 / Formula: LMN
Molecular weightTheoretical: 1.005188 KDa
Chemical component information

ChemComp-AV0:
Lauryl Maltose Neopentyl Glycol

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 %

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.78 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 105000
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.27 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 152134
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: PROJECTION MATCHING
FSC plot (resolution estimation)

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