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- EMDB-65908: Cryo-EM structure of helicase DruE in the Druantia anti-phage sys... -

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Basic information

Entry
Database: EMDB / ID: EMD-65908
TitleCryo-EM structure of helicase DruE in the Druantia anti-phage system elucidates its anti-phage mechanism
Map data
Sample
  • Complex: DruE-DNA-AMPPNP complex
    • Protein or peptide: Helicase
    • DNA: DNA (70-MER)
  • Ligand: ZINC ION
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
KeywordsSF2 Helicase / DNA-dependent ATPase / DNA-binding / DNA BINDING PROTEIN / HYDROLASE
Function / homology
Function and homology information


3'-5' DNA helicase activity / interstrand cross-link repair / nucleotide-excision repair / nucleic acid binding / ATP binding
Similarity search - Function
YjiV N-terminal domain / DEAD/DEAH box helicase domain / DEAD/DEAH box helicase / Helicase conserved C-terminal domain / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Biological speciesPseudomonas protegens Pf-5 (bacteria) / synthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.36 Å
AuthorsHou J / He YX / Gui L
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31971422 China
CitationJournal: To Be Published
Title: Cryo-EM structure of helicase DruE in the Druantia anti-phage system elucidates its anti-phage mechanism
Authors: Hou J / He YX / Gui L
History
DepositionAug 19, 2025-
Header (metadata) releaseAug 26, 2026-
Map releaseAug 26, 2026-
UpdateAug 26, 2026-
Current statusAug 26, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_65908.png

Downloads & links

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Map

FileDownload / File: emd_65908.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.96 Å/pix.
x 400 pix.
= 384. Å		0.96 Å/pix.
x 400 pix.
= 384. Å		0.96 Å/pix.
x 400 pix.
= 384. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.96 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 1.77
Minimum - Maximum-0.094401464 - 26.517315
Average (Standard dev.)0.0041472954 (±0.5036124)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 384.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_65908_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_65908_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_65908_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : DruE-DNA-AMPPNP complex

EntireName: DruE-DNA-AMPPNP complex
Components
  • Complex: DruE-DNA-AMPPNP complex
    • Protein or peptide: Helicase
    • DNA: DNA (70-MER)
  • Ligand: ZINC ION
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

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Supramolecule #1: DruE-DNA-AMPPNP complex

SupramoleculeName: DruE-DNA-AMPPNP complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Pseudomonas protegens Pf-5 (bacteria)
Molecular weightTheoretical: 463 KDa

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Macromolecule #1: Helicase

MacromoleculeName: Helicase / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas protegens Pf-5 (bacteria)
Molecular weightTheoretical: 221.376172 KDa
Recombinant expressionOrganism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)
SequenceString: MGSSHHHHHH SQDPMVLDPI GGFHRIQDFF ISYVETSFRI SNPTAAEARR ALLKTCGILA TEPFIEPVLR YESSDKNLED LIEEEDGTL QPLSLEGRKA FVELALSGLF DGEPSKGLLR RKSAYAPYRH QISMLERGVR AGCPGIVTSG TGSGKTESFM L PVLAALAN ...String:
MGSSHHHHHH SQDPMVLDPI GGFHRIQDFF ISYVETSFRI SNPTAAEARR ALLKTCGILA TEPFIEPVLR YESSDKNLED LIEEEDGTL QPLSLEGRKA FVELALSGLF DGEPSKGLLR RKSAYAPYRH QISMLERGVR AGCPGIVTSG TGSGKTESFM L PVLAALAN EAVDWPKPGV NYLQEHWWQT PKSRWSPRRA GEKRPAAVRA LVLYPMNALV EDQMVRLRKT LDAEEAHAVM DE RFTGNRL FFGQYTSATP VTGYEQHPRL AGDKQEVKRR ARRIARLRKA MQNFQRDQDA ARRFDADVQS DGKASVEKTR YIF PSVDGG EMVSRWDMHA APPDILVTNA SMLGAMLSRE IEDAIFEKTR EWLMSDEDAY FYLIFDELHL IRGSAGTEIA LLIK SLIQR LGLDQPEHCY KLRLLASSAS LPMEGVEGVQ SRTYLRDLFA PFGTSSRPND LGSIDPSFWS KCIIQGVVHI PPVQQ CGIP AEPFVQLMKA ALEGKDNFVG QLDRTPALDA AIVQAAKVLG ITETEQTTLV KQLAETAASL LTHACKNDGT IRATTP RSI AARIFAPATG DTELALRGLL LARSLPESNQ SAVKVAVATP AFRVHTFIRN IEGLFASVAP GEPDVAFDNF SVERGTS HS APVEGQRRGH RLFELLYCEA CGDLFVGGQR GQSSGSMNAT ELLPSAANLE HLPERPGAEY YDDMTLDEFA VFWPRRGD W IGSDKGYDQW EPAHLNPDTG IVEIGEIAKE GNIAGYLYYQ TKAAVSKDKE RSTQQPSAQP FCCPKCGTDY SNRPDTNRS RSPIRAFRTG VTKSSQLVAT ELFELLHAIG AEPKGIVFSD SRQDAAAQAM EIERLHLRDL RREVLVTAAR SYVKDASIEV LTSKEIIER LIKAETAGDK DEVIRLSELL KKQTVACGPK TRKVKLDKLL EFGNDGSIGR ITAELVRLGI SPYKHSSSND A KELPWYRE FEKNGDLIAY DHTLSYTQTV GLNKDIVEGQ YELIDDVIFA NTFFALEETG LAYPCLPMDE DDQALDAWLR VF ASAYRVK DNRYFDKNKV KLWQKGPDVT NGRIKKVARA LYGETRYGDE LTRVLGEFER LGHRGGLFNI GKLALKVTEP GDD FWRCSN CERVHLHRGL GVCTRCVAPL HEPSGKVEAL WETNFLGRRI VRGERDGVGR FRLRVEELTG QTDDFSDRLR KFKG IFVDD VSELEKLASE IDMLSVTTTM EVGIDIGALQ TVYQANMPPQ RFNYQQRVGR AGRRGQAFSF VVTFCRGRSH DAYYF AHPQ AITGDPPPPP FLATGHDAIP MRLLRKTWLR AAFKQLREQC AKLGESFPGD LLIPPDVHGE YVTTKDYYHS QEIDWP IRL SEALKQTQSV RDRFIETATF DQEQRQRLYA KSSIDLLLKE INDQRPHAPD DEVGLARFLA ERGLLPMYGM PTRVRNL YV GLRESKTQSD HSEYEWSMMD RDLDLAVFEY APGAVLVKDK KKHRVIGFTG NLTDPQAQGR SIEGIRSVTD WSESQTYV A ICPACGSASQ SKQAPEGPLA CNDCQAPIPK ESFLHYVTPA AFRTDFLPKD ELDEFERMSL RTVATVLREG DCFNYRALT VRSGAGVTIL QLNDGPTDGK NDGQRFTVDL VQDQRVPVPF STQRPAIDGI QAIESSWRRS HISPRWSQPL SEQRFGLISQ KETDSIHLE LTRFDKRLTL DMVSRKGDFM HLPTRAAAIS ATQILVNKAA LALDVSPDEF EALEPRLRSG HPMLQIADAL I NGSGLSRR LGEPASDGPT PLLVDLLHEI LEKPNVWPLQ DFLRTGAEGP HAAQCQTSCY RCIQRYGNRR YHGLLDWRLG LA YLRTLVT PAYACGLTPG DDKYPEIQGW RERASQLADD VEAMRKGTIR TERLPHSDLP CLIEQKDGVE LWRAVVIHPL WRH SEPGVM RDLLGADWSP SLRYLDTFEL ERRPLRRLAA LKQEGSWSHP QFEK

UniProtKB: Helicase

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Macromolecule #2: DNA (70-MER)

MacromoleculeName: DNA (70-MER) / type: dna / ID: 2 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 21.49073 KDa
SequenceString: (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DT) (DC)(DG)(DA)(DT)(DG)(DA)(DG)(DC)(DA)(DC) (DT)(DG)(DC)(DT)(DA)(DT)(DT)(DC)(DC) (DC)(DT)(DA)(DG)(DC)(DA)(DG)(DT)(DG)(DC) (DT) (DC)(DA)(DT)(DC)(DG)(DA) ...String:
(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DT) (DC)(DG)(DA)(DT)(DG)(DA)(DG)(DC)(DA)(DC) (DT)(DG)(DC)(DT)(DA)(DT)(DT)(DC)(DC) (DC)(DT)(DA)(DG)(DC)(DA)(DG)(DT)(DG)(DC) (DT) (DC)(DA)(DT)(DC)(DG)(DA)(DT)(DG) (DA)(DT)(DT)(DT)(DT)(DC)(DA)(DT)(DC)(DG) (DA)(DT) (DG)(DA)(DG)(DC)(DG)(DG)(DT) (DT)(DT)(DT)

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Macromolecule #3: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 3 / Number of copies: 8 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #4: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 4 / Number of copies: 1 / Formula: ANP
Molecular weightTheoretical: 506.196 Da
Chemical component information

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.5 mg/mL
BufferpH: 8
Component:
ConcentrationFormulaName
150.0 mM/LNaClsodium chloride
20.0 mM/LTris-HclTRIS hydrochloride

Details: 150mM NaCl,20mM Tris-HCL
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.04 kPa
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 130000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.36 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 136706
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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