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- EMDB-65778: Cryo-EM structure of the kinesin-2 tail domain in complex with KA... -

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Basic information

Entry
Database: EMDB / ID: EMD-65778
TitleCryo-EM structure of the kinesin-2 tail domain in complex with KAP3 and APC
Map datasharpened map
Sample
  • Complex: Tetrameric complex of kinesin-2 KIF3A/B/KAP3 and cargo APC
    • Protein or peptide: Kinesin-like protein KIF3A
    • Protein or peptide: Kinesin-like protein KIF3B, N-terminally processed
    • Protein or peptide: Kinesin-associated protein 3
    • Protein or peptide: Adenomatous polyposis coli protein
KeywordsKinesin-2 motor Intracellular transport Kinesin-adaptor-cargo complex / MOTOR PROTEIN
Function / homology
Function and homology information


negative regulation of odontogenesis / canonical Wnt signaling pathway involved in negative regulation of apoptotic process / canonical Wnt signaling pathway involved in positive regulation of apoptotic process / negative regulation of acinar cell proliferation / axis specification / positive regulation of calcium-dependent cell-cell adhesion / opsin transport / epidermal stem cell homeostasis / kinesin II complex / anterograde dendritic transport ...negative regulation of odontogenesis / canonical Wnt signaling pathway involved in negative regulation of apoptotic process / canonical Wnt signaling pathway involved in positive regulation of apoptotic process / negative regulation of acinar cell proliferation / axis specification / positive regulation of calcium-dependent cell-cell adhesion / opsin transport / epidermal stem cell homeostasis / kinesin II complex / anterograde dendritic transport / intraciliary transport particle B binding / Apoptotic cleavage of cellular proteins / periciliary membrane compartment / acinar cell proliferation / Activation of SMO / Beta-catenin phosphorylation cascade / Intraflagellar transport / Disassembly of the destruction complex and recruitment of AXIN to the membrane / nitrogen cycle metabolic process / centriole-centriole cohesion / Deactivation of the beta-catenin transactivating complex / Ovarian tumor domain proteases / anterograde dendritic transport of neurotransmitter receptor complex / positive regulation of keratinocyte proliferation / Kinesins / regulation of epithelial cell differentiation / microtubule anchoring at centrosome / negative regulation of cell cycle G1/S phase transition / intraciliary transport / Degradation of beta-catenin by the destruction complex / cilium movement / Scrib-APC-beta-catenin complex / photoreceptor connecting cilium / inner ear receptor cell stereocilium organization / COPI-dependent Golgi-to-ER retrograde traffic / motile cilium assembly / proximal/distal pattern formation / negative regulation of epithelial cell proliferation involved in prostate gland development / positive regulation of fibroblast apoptotic process / regulation of epithelial cell migration / negative regulation of cyclin-dependent protein serine/threonine kinase activity / regulation of attachment of spindle microtubules to kinetochore / Hedgehog 'off' state / positive regulation of pseudopodium assembly / metaphase/anaphase transition of mitotic cell cycle / positive regulation of protein localization to centrosome / regulation of osteoclast differentiation / dorsal/ventral neural tube patterning / cell projection membrane / pattern specification process / fibroblast migration / negative regulation of epithelial cell apoptotic process / dentate gyrus development / negative regulation of microtubule depolymerization / epithelial cell proliferation involved in prostate gland development / beta-catenin destruction complex / MHC class II antigen presentation / regulation of microtubule-based process / microtubule plus-end binding / non-motile cilium assembly / negative regulation of thymocyte apoptotic process / dorsal/ventral pattern formation / protein kinase regulator activity / endothelial cell proliferation / determination of left/right symmetry / microtubule depolymerization / fibroblast apoptotic process / neural precursor cell proliferation / positive regulation of fibroblast migration / epithelial cell apoptotic process / odontogenesis / microtubule motor activity / anterior/posterior pattern specification / neural tube development / negative regulation of cardiac muscle cell apoptotic process / motile cilium / negative regulation of neuroblast proliferation / smoothened signaling pathway / skin development / regulation of osteoblast differentiation / stem cell population maintenance / spectrin binding / muscle cell cellular homeostasis / thymocyte apoptotic process / mitotic spindle assembly checkpoint signaling / positive regulation of cytokinesis / heart looping / negative regulation of endothelial cell proliferation / negative regulation of Wnt signaling pathway / forebrain development / dynein complex binding / microtubule polymerization / regulation of cell differentiation / mitotic cytokinesis / positive regulation of cell division / lateral plasma membrane / kinesin binding / keratinocyte proliferation / axoneme / cilium assembly
Similarity search - Function
Kinesin-associated protein 3 / Kinesin-associated protein (KAP) / Kinesin-associated protein (KAP) / Adenomatous polyposis coli protein repeat / SAMP / EB-1 binding / Adenomatous polyposis coli protein basic domain / Adenomatous polyposis coli protein, 15 residue repeat / Adenomatous polyposis coli (APC) family / Adenomatous polyposis coli protein ...Kinesin-associated protein 3 / Kinesin-associated protein (KAP) / Kinesin-associated protein (KAP) / Adenomatous polyposis coli protein repeat / SAMP / EB-1 binding / Adenomatous polyposis coli protein basic domain / Adenomatous polyposis coli protein, 15 residue repeat / Adenomatous polyposis coli (APC) family / Adenomatous polyposis coli protein / Adenomatous polyposis coli, N-terminal dimerisation domain / APC, N-terminal coiled-coil domain superfamily / Adenomatous polyposis coli (APC) repeat / APC repeat / SAMP Motif / EB-1 Binding Domain / APC basic domain / APC 15 residue motif / Adenomatous polyposis coli tumour suppressor protein / Armadillo-associated region on APC / Unstructured region on APC between 1st and 2nd catenin-bdg motifs / Unstructured region on APC between 1st two creatine-rich regions / Unstructured region on APC between APC_crr and SAMP / Unstructured region on APC between SAMP and APC_crr / Unstructured region on APC between APC_crr regions 5 and 6 / Coiled-coil N-terminus of APC, dimerisation domain / Adenomatous polyposis coli (APC) repeat / Kinesin-like protein / Armadillo/plakoglobin ARM repeat profile. / Armadillo/beta-catenin-like repeat / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor domain / Kinesin motor domain profile. / Kinesin motor, catalytic domain. ATPase. / Kinesin motor domain / Armadillo/beta-catenin-like repeats / Armadillo / Kinesin motor domain superfamily / Armadillo-like helical / Armadillo-type fold / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Kinesin-like protein KIF3A / Kinesin-associated protein 3 / Adenomatous polyposis coli protein / Kinesin-like protein KIF3B
Similarity search - Component
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsJiang X / Danev R / Yanagisawa H / Kikkawa M
Funding support Japan, 4 items
OrganizationGrant numberCountry
Japan Science and TechnologyJPMJER2202 Japan
Japan Society for the Promotion of Science (JSPS)JP21H05247 Japan
Japan Society for the Promotion of Science (JSPS)JP24KF0141 Japan
Japan Society for the Promotion of Science (JSPS)JP24K18106 Japan
CitationJournal: To Be Published
Title: The Hook-like Adaptor and Cargo-binding (HAC) Domain in the Kinesin-2 Tail Enables Adaptor Assembly and Cargo Recognition
Authors: Jiang X / Danev R / Yanagisawa H / Kikkawa K
History
DepositionAug 10, 2025-
Header (metadata) releaseOct 1, 2025-
Map releaseOct 1, 2025-
UpdateOct 1, 2025-
Current statusOct 1, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_65778.png

Downloads & links

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Map

FileDownload / File: emd_65778.map.gz / Format: CCP4 / Size: 15.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationsharpened map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.3 Å/pix.
x 160 pix.
= 208. Å		1.3 Å/pix.
x 160 pix.
= 208. Å		1.3 Å/pix.
x 160 pix.
= 208. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.3 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.007
Minimum - Maximum-0.0017263165 - 2.4871056
Average (Standard dev.)0.0019050711 (±0.029757828)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions160160160
Spacing160160160
CellA=B=C: 208.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: raw map

Fileemd_65778_additional_1.map
Annotationraw map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: halfmap2

Fileemd_65778_half_map_1.map
Annotationhalfmap2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: halfmap1

Fileemd_65778_half_map_2.map
Annotationhalfmap1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Tetrameric complex of kinesin-2 KIF3A/B/KAP3 and cargo APC

EntireName: Tetrameric complex of kinesin-2 KIF3A/B/KAP3 and cargo APC
Components
  • Complex: Tetrameric complex of kinesin-2 KIF3A/B/KAP3 and cargo APC
    • Protein or peptide: Kinesin-like protein KIF3A
    • Protein or peptide: Kinesin-like protein KIF3B, N-terminally processed
    • Protein or peptide: Kinesin-associated protein 3
    • Protein or peptide: Adenomatous polyposis coli protein

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Supramolecule #1: Tetrameric complex of kinesin-2 KIF3A/B/KAP3 and cargo APC

SupramoleculeName: Tetrameric complex of kinesin-2 KIF3A/B/KAP3 and cargo APC
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 220 KDa

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Macromolecule #1: Kinesin-like protein KIF3A

MacromoleculeName: Kinesin-like protein KIF3A / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 21.084143 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MGSSHHHHHH SQSLQEEAQG KTKKLKKVWT MLMAAKSEMA DLQQEHQREI EGLLENIRQL SRELRLQMLI IDNFIPQDYQ EMIENYVHW NEDIGEWQLK CVAYTGNNMR KQTPVPDKKE RDPFEVDLSH VYLAYTEESL RQSLMKLERP RTSKGKARPK M GRRKRSAK PETVIDSLLQ

UniProtKB: Kinesin-like protein KIF3A

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Macromolecule #2: Kinesin-like protein KIF3B, N-terminally processed

MacromoleculeName: Kinesin-like protein KIF3B, N-terminally processed / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 25.440057 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MSLQQEVDIK TKKLKKLFSK LQAVKAEIHD LQEEHIKERQ ELEQTQNELT RELKLKHLII ENFIPLEEKN KIMNRSFFDD EEDHWKLHP ITRLENQQMM KRPVSAVGYK RPLSQHARMS MMIRPEPRYR AENIMLLELD MPSRTTRDYE GPAISPKVQA A LDAALQDE ...String:
MSLQQEVDIK TKKLKKLFSK LQAVKAEIHD LQEEHIKERQ ELEQTQNELT RELKLKHLII ENFIPLEEKN KIMNRSFFDD EEDHWKLHP ITRLENQQMM KRPVSAVGYK RPLSQHARMS MMIRPEPRYR AENIMLLELD MPSRTTRDYE GPAISPKVQA A LDAALQDE DEIQVDASSF ESTASRKPKA RPKSGRKSGS SSSSSGNPAS QFYPQSRGLV PK

UniProtKB: Kinesin-like protein KIF3B

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Macromolecule #3: Kinesin-associated protein 3

MacromoleculeName: Kinesin-associated protein 3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 80.224148 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MQGEDARYLK RKVKGGNIDV HPSEKALIVQ YEVEATILGE MGDPMLGERK ECQKIIRLKS LNANTDITSL ARKVVEECKL IHPSKLSEV EQLLYYLQNR RDSLPGKEKK EKSSKPKDPP PFEGMEIDEV ANINDMDEYI ELLYEDIPDK VRGSALILQL A RNPDNLEE ...String:
MQGEDARYLK RKVKGGNIDV HPSEKALIVQ YEVEATILGE MGDPMLGERK ECQKIIRLKS LNANTDITSL ARKVVEECKL IHPSKLSEV EQLLYYLQNR RDSLPGKEKK EKSSKPKDPP PFEGMEIDEV ANINDMDEYI ELLYEDIPDK VRGSALILQL A RNPDNLEE LLLNETALGA LARVLREDWK QSVELATNII YIFFCFSSFS HFHGLITHYK IGALCMNIID HELKRHELWQ EE LSKKKKA VDEDLENQTL RKDYDKTFKK YQGLVVKQEQ LLRVALYLLL NLAEDTRTEL KMRNKNIVHM LVKALDRDNF ELL ILVVSF LKKLSIFMEN KNDMVEMDIV EKLVKMIPCE HEDLLNITLR LLLNLSFDTG LRNKMVQVGL LPKLTALLGN ENYK QIAMC VLYHISMDDR FKSMFAYTDC IPQLMKMLFE CSDERIDLEL ISFCINLAAN KRNVQLICEG NGLKMLMKRA LKLKD PLLM KMIRNISQHD GPTKNLFIDY VGDLAAQISS DEEEEFVIEC LGTLANLTIP DLDWELVLKE YKLVPFLKDK LKPGAA EDD LVLEVVIMIG TVSMDDSCAA LLAKSGIIPA LIELLNAQQE DDEFVCQIIY VFYQMVFHQA TRDVIIKETQ APAYLID LM HDKNNEIRKV CDNTLDIIAE YDEEWAKKIQ SEKFRWHNSQ WLEMVESRQL D

UniProtKB: Kinesin-associated protein 3

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Macromolecule #4: Adenomatous polyposis coli protein

MacromoleculeName: Adenomatous polyposis coli protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 75.417633 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MHHHHHHSQD SCISMRQSGC LPLLIQLLHG NDKDSVLLGN SRGSKEARAR ASAALHNIIH SQPDDKRGRR EIRVLHLLEQ IRAYCETCW EWQEAHEQGM DQDKNPMPAP VEHQICPAVC VLMKLSFDEE HRHAMNELGG LQAIAELLQV DCEMYGLTND H YSVTLRRY ...String:
MHHHHHHSQD SCISMRQSGC LPLLIQLLHG NDKDSVLLGN SRGSKEARAR ASAALHNIIH SQPDDKRGRR EIRVLHLLEQ IRAYCETCW EWQEAHEQGM DQDKNPMPAP VEHQICPAVC VLMKLSFDEE HRHAMNELGG LQAIAELLQV DCEMYGLTND H YSVTLRRY AGMALTNLTF GDVANKATLC SMKGCMRALV AQLKSESEDL QQVIASVLRN LSWRADVNSK KTLREVGSVK AL MECALEV KKESTLKSVL SALWNLSAHC TENKADICAV DGALAFLVGT LTYRSQTNTL AIIESGGGIL RNVSSLIATN EDH RQILRE NNCLQTLLQH LKSHSLTIVS NACGTLWNLS ARNPKDQEAL WDMGAVSMLK NLIHSKHKMI AMGSAAALRN LMAN RPAKY KDANIMSPGS SLPSLHVRKQ KALEAELDAQ HLSETFDNID NLSPKASHRS KQRHKQNLYG DYAFDANRHD DSRSD NFNT GNMTVLSPYL NTTVLPSSSS SRGSLDSSRS EKDRSLERER GIGLSAYHPT TENAGTSSKR GLQITTTAAQ IAKVME EVS AIHTSQDDRS SASTTEFHCV ADDRSAARRS SASHTHSNTY NFTKSENSNR TCSMPYAKVE YKRSSNDSLN SVTSSDG YG KRGQMKPSVE SYSEDDESKF CSYGQYPADL AHKIHSAN

UniProtKB: Adenomatous polyposis coli protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.0375 mg/mL
BufferpH: 8
GridModel: Quantifoil R1.2/1.3 / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 120 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 279 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy #1

Microscopy ID1
MicroscopeJEOL CRYO ARM 200
Specialist opticsEnergy filter - Slit width: 20 eV
Image recordingImage recording ID: 1 / Film or detector model: GATAN K3 (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number grids imaged: 1 / Number real images: 36020 / Average electron dose: 64.7 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 1.55 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 80000
Sample stageCooling holder cryogen: NITROGEN

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Electron microscopy #1~

Microscopy ID1
MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Slit width: 20 eV
Image recordingImage recording ID: 2 / Film or detector model: GATAN K3 (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number grids imaged: 1 / Number real images: 19918 / Average electron dose: 63.0 e/Å2
Details: tilted-stage image collection with tilt angles of 30 and 40 degrees
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.6 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 130000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Image recording ID1
Particle selectionNumber selected: 12833027
CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER / Details: Ab-Initio Reconstruction
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 407310
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final 3D classificationNumber classes: 4 / Software - Name: cryoSPARC
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model
PDB IDChainDetails

9w9h

source_name: PDB, initial_model_type: experimental modelchains, A-C

4yje

source_name: PDB, initial_model_type: experimental modelchain D
DetailsInitial local fitting was done using Phenix dock-in-map tool.
RefinementSpace: REAL / Protocol: OTHER
Output model

PDB-9w9i:
Cryo-EM structure of the kinesin-2 tail domain in complex with KAP3 and APC

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