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- EMDB-65612: Cryo-EM structure of human papillomavirus type 45 in complexed wi... -

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Basic information

Entry
Database: EMDB / ID: EMD-65612
TitleCryo-EM structure of human papillomavirus type 45 in complexed with the Fab fragment of 20B8
Map data
Sample
  • Complex: Cryo-EM structure of human papillomavirus type 45 in complexed with the Fab fragment of 20B8
    • Complex: Human papillomavirus type 45
    • Complex: The Fab fragment of 20B8
KeywordsHuman papillomavirus / Cryo-EM / Structural protein / VIRUS LIKE PARTICLE
Biological speciesHuman papillomavirus / Mus (mice)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.78 Å
AuthorsJiang Y / Sun H / Wang Z / Zheng Q / Li S
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Structure / Year: 2026
Title: Cryo-EM structure of human papillomavirus type 45 in complexed with the Fab fragment of 10G2
Authors: Jiang Y / Sun H / Wang Z / Zheng Q / Li S / Xia N
History
DepositionJul 30, 2025-
Header (metadata) releaseFeb 11, 2026-
Map releaseFeb 11, 2026-
UpdateFeb 11, 2026-
Current statusFeb 11, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_65612.png

Downloads & links

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Map

FileDownload / File: emd_65612.map.gz / Format: CCP4 / Size: 1.7 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.12 Å/pix.
x 768 pix.
= 860.16 Å		1.12 Å/pix.
x 768 pix.
= 860.16 Å		1.12 Å/pix.
x 768 pix.
= 860.16 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.12 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.4
Minimum - Maximum-0.33160812 - 1.0906061
Average (Standard dev.)0.031065801 (±0.16857378)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions768768768
Spacing768768768
CellA=B=C: 860.16003 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_65612_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_65612_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Cryo-EM structure of human papillomavirus type 45 in complexed wi...

EntireName: Cryo-EM structure of human papillomavirus type 45 in complexed with the Fab fragment of 20B8
Components
  • Complex: Cryo-EM structure of human papillomavirus type 45 in complexed with the Fab fragment of 20B8
    • Complex: Human papillomavirus type 45
    • Complex: The Fab fragment of 20B8

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Supramolecule #1: Cryo-EM structure of human papillomavirus type 45 in complexed wi...

SupramoleculeName: Cryo-EM structure of human papillomavirus type 45 in complexed with the Fab fragment of 20B8
type: complex / ID: 1 / Parent: 0

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Supramolecule #2: Human papillomavirus type 45

SupramoleculeName: Human papillomavirus type 45 / type: complex / ID: 2 / Parent: 1
Source (natural)Organism: Human papillomavirus

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Supramolecule #3: The Fab fragment of 20B8

SupramoleculeName: The Fab fragment of 20B8 / type: complex / ID: 3 / Parent: 1
Source (natural)Organism: Mus (mice)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TECNAI F30
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 32.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.8000000000000003 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Tecnai F30 / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 5.78 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 6674
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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