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- EMDB-65475: Cryo-EM structure of the head of contracted P1 -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-65475
TitleCryo-EM structure of the head of contracted P1
Map data
Sample
  • Complex: Escherichia phage P1
    • Protein or peptide: Hdf
    • Protein or peptide: Defense against restriction protein A
Keywordshead / contracted phage P1 / VIRAL PROTEIN
Function / homology
Function and homology information


symbiont-mediated evasion of host restriction-modification system / viral capsid assembly / viral capsid / symbiont-mediated suppression of host innate immune response
Similarity search - Function
Defence against restriction A, N-terminal / Defence against restriction A, C-terminal / Defence against restriction A N-terminal / Defence against restriction A C-terminal
Similarity search - Domain/homology
Defense against restriction protein A / Hdf
Similarity search - Component
Biological speciesEscherichia phage P1 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.0 Å
AuthorsChen Y / Liu HR
Funding support China, 3 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)12034006 China
National Natural Science Foundation of China (NSFC)32430020 China
National Natural Science Foundation of China (NSFC)32071209 China
CitationJournal: To Be Published
Title: The in stiu structure of the head of contracted P1
Authors: Chen Y / Liu HR
History
DepositionJul 21, 2025-
Header (metadata) releaseOct 22, 2025-
Map releaseOct 22, 2025-
UpdateOct 22, 2025-
Current statusOct 22, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_65475.png

Downloads & links

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Map

FileDownload / File: emd_65475.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.36 Å/pix.
x 400 pix.
= 544. Å		1.36 Å/pix.
x 400 pix.
= 544. Å		1.36 Å/pix.
x 400 pix.
= 544. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.36 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 2.4
Minimum - Maximum-5.6673074 - 16.288533999999999
Average (Standard dev.)0.0044870824 (±0.88421345)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-200-200-200
Dimensions400400400
Spacing400400400
CellA=B=C: 544.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_65475_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_65475_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Escherichia phage P1

EntireName: Escherichia phage P1 (virus)
Components
  • Complex: Escherichia phage P1
    • Protein or peptide: Hdf
    • Protein or peptide: Defense against restriction protein A

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Supramolecule #1: Escherichia phage P1

SupramoleculeName: Escherichia phage P1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Escherichia phage P1 (virus)

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Macromolecule #1: Hdf

MacromoleculeName: Hdf / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage P1 (virus)
Molecular weightTheoretical: 22.189506 KDa
SequenceString: MCQMTKNKYA TVDFDQVNEK GLKSLITAIN KTGVTVIEVD SSNRATTKDG VKVKTAKLVL SDGQILAIQV NDTGDISSVK LNGKAIPNA QSPDIKTLGT VMGQAARKNS AKFQKSLIAK AKRVANPVDK KPAVKSNFQR LQEAKQRNAQ VVAAYKSAQN S VSFNQQQI ...String:
MCQMTKNKYA TVDFDQVNEK GLKSLITAIN KTGVTVIEVD SSNRATTKDG VKVKTAKLVL SDGQILAIQV NDTGDISSVK LNGKAIPNA QSPDIKTLGT VMGQAARKNS AKFQKSLIAK AKRVANPVDK KPAVKSNFQR LQEAKQRNAQ VVAAYKSAQN S VSFNQQQI TDLRAKLDKE TGRLNNEKAR NGELKRRLKQ LKAGN

UniProtKB: Hdf

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Macromolecule #2: Defense against restriction protein A

MacromoleculeName: Defense against restriction protein A / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage P1 (virus)
Molecular weightTheoretical: 69.550734 KDa
SequenceString: MEQFNINKGM TIKPGLDVLP PPVTDDEYRA LMAGEDRYLM TESNTLEEIE ATFFYDTPIH WCATDLLEAI SSTRLQLHRT MQAFVRALN QKLNGTGISA GSDKTGDVAQ SGARAIGGAE IGRARNVNGL PVLPAIIPLS DGQTISILFH SPTAENRITN S DTLVAFQF ...String:
MEQFNINKGM TIKPGLDVLP PPVTDDEYRA LMAGEDRYLM TESNTLEEIE ATFFYDTPIH WCATDLLEAI SSTRLQLHRT MQAFVRALN QKLNGTGISA GSDKTGDVAQ SGARAIGGAE IGRARNVNGL PVLPAIIPLS DGQTISILFH SPTAENRITN S DTLVAFQF LLNKKDVTHT VAPMSGRDMT LAQVTMKLAN LAEKNSAKFQ RAQKKKKALV DEITQLQADS DQKEDAMSDL AD QVAAVEG QKADLEQKIN AVASEADSLY EENERLQGEI DRLNRTGGRD TIAPAGMTGG HSRALTDRLA SIKNRMHMDG EAT LSNGAS MKQFIGDGEG YIQLTDPDGS VYMIKAKSIQ GVDMADAIGK LFKAYKAGNV SEYLVQPEEH KPENVEPESA EDTG SSSPE PEVSVGAYRY ALQMRPAAPG AIPEGNKAIL PRPDEGDPYY EYARYGIATY DTPLSDQQMS EYDLKLLPRE DSFDF LAKT LTNGPFGKYA QKALELATNS PDEFRVMLKT QFQKTFPNIA FPGGAGTEKM VQSMINALQA EVGEITQPEP APAQPD ETV SEADAEANKA IEYLNNVMDM QSTDMAEIRN ARGNVREAIA ALQTAGRFEE NEELVNGAAR HLADLLVAIQ KAGVAA

UniProtKB: Defense against restriction protein A

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 32.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: EMAN / Number images used: 3200
Initial angle assignmentType: COMMON LINE
Final angle assignmentType: COMMON LINE

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