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Yorodumi- EMDB-64645: EMCV IRES captured on mammalian 40S ribosome with initiator tRNA ... -
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Open data
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Basic information
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| Title | EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 | |||||||||
Map data | ||||||||||
Sample |
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Keywords | EMCV IRES / 40S ribosome / 48S PIC / RIBOSOME | |||||||||
| Function / homology | Function and homology informationlaminin receptor activity / 90S preribosome / ubiquitin ligase inhibitor activity / positive regulation of signal transduction by p53 class mediator / phagocytic cup / translation regulator activity / rough endoplasmic reticulum / ribosomal small subunit export from nucleus / laminin binding / gastrulation ...laminin receptor activity / 90S preribosome / ubiquitin ligase inhibitor activity / positive regulation of signal transduction by p53 class mediator / phagocytic cup / translation regulator activity / rough endoplasmic reticulum / ribosomal small subunit export from nucleus / laminin binding / gastrulation / MDM2/MDM4 family protein binding / cytosolic ribosome / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA / small-subunit processome / spindle / rRNA processing / positive regulation of canonical Wnt signaling pathway / rhythmic process / regulation of translation / ribosomal small subunit assembly / virus receptor activity / ribosome binding / ribosomal small subunit biogenesis / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / perikaryon / cell differentiation / cytoplasmic translation / mitochondrial inner membrane / postsynaptic density / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / cell division / DNA repair / mRNA binding / apoptotic process / centrosome / synapse / dendrite / nucleolus / perinuclear region of cytoplasm / Golgi apparatus / DNA binding / RNA binding / zinc ion binding / membrane / nucleus / plasma membrane / cytoplasm Similarity search - Function | |||||||||
| Biological species | ![]() Encephalomyocarditis virus | |||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 5.01 Å | |||||||||
Authors | Das D / Hussain T | |||||||||
| Funding support | India, 1 items
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Citation | Journal: Elife / Year: 2026Title: Structural insights into the recruitment of viral Type 2 IRES to ribosomal preinitiation complex for protein synthesis Authors: Das D / Hussain T | |||||||||
| History |
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Structure visualization
| Supplemental images |
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Downloads & links
-EMDB archive
| Map data | emd_64645.map.gz | 230.2 MB | EMDB map data format | |
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| Header (meta data) | emd-64645-v30.xml emd-64645.xml | 58.2 KB 58.2 KB | Display Display | EMDB header |
| FSC (resolution estimation) | emd_64645_fsc.xml | 13.3 KB | Display | FSC data file |
| Images | emd_64645.png | 58.7 KB | ||
| Filedesc metadata | emd-64645.cif.gz | 13.1 KB | ||
| Others | emd_64645_half_map_1.map.gz emd_64645_half_map_2.map.gz | 226.5 MB 226.5 MB | ||
| Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-64645 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-64645 | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 9uzlMC ![]() 9uzkC ![]() 9uzmC C: citing same article ( M: atomic model generated by this map |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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| Related items in Molecule of the Month |
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Map
| File | Download / File: emd_64645.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 1.17 Å | ||||||||||||||||||||||||||||||||||||
| Density |
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| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML:
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-Supplemental data
-Half map: #2
| File | emd_64645_half_map_1.map | ||||||||||||
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| Density Histograms |
-Half map: #1
| File | emd_64645_half_map_2.map | ||||||||||||
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| Density Histograms |
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Sample components
+Entire : EMCV IRES with 40S and initiator tRNA
+Supramolecule #1: EMCV IRES with 40S and initiator tRNA
+Macromolecule #1: 18S ribosomal RNA
+Macromolecule #38: Initiator tRNA
+Macromolecule #39: Encephalomyocarditis viral IRES
+Macromolecule #2: Eukaryotic translation initiation factor 2 subunit 1
+Macromolecule #3: Eukaryotic translation initiation factor 2 subunit 3
+Macromolecule #4: Small ribosomal subunit protein uS2
+Macromolecule #5: Small ribosomal subunit protein eS1
+Macromolecule #6: Small ribosomal subunit protein uS5
+Macromolecule #7: Small ribosomal subunit protein uS3
+Macromolecule #8: Small ribosomal subunit protein eS4
+Macromolecule #9: Small ribosomal subunit protein uS7
+Macromolecule #10: Small ribosomal subunit protein eS6
+Macromolecule #11: Small ribosomal subunit protein eS7
+Macromolecule #12: Small ribosomal subunit protein eS8
+Macromolecule #13: Small ribosomal subunit protein uS4
+Macromolecule #14: Small ribosomal subunit protein eS10
+Macromolecule #15: Small ribosomal subunit protein uS17
+Macromolecule #16: Small ribosomal subunit protein eS12
+Macromolecule #17: Small ribosomal subunit protein uS15
+Macromolecule #18: Ribosomal protein S14
+Macromolecule #19: Small ribosomal subunit protein uS19
+Macromolecule #20: Small ribosomal subunit protein uS9
+Macromolecule #21: Small ribosomal subunit protein eS17
+Macromolecule #22: Small ribosomal subunit protein uS13
+Macromolecule #23: Small ribosomal subunit protein eS19
+Macromolecule #24: Small ribosomal subunit protein uS10
+Macromolecule #25: Small ribosomal subunit protein eS21
+Macromolecule #26: Small ribosomal subunit protein uS8
+Macromolecule #27: Small ribosomal subunit protein uS12
+Macromolecule #28: 40S ribosomal protein S24
+Macromolecule #29: Small ribosomal subunit protein eS26
+Macromolecule #30: Small ribosomal subunit protein eS27
+Macromolecule #31: Small ribosomal subunit protein eS28
+Macromolecule #32: Small ribosomal subunit protein uS14
+Macromolecule #33: Ubiquitin-ribosomal protein eS31 fusion protein
+Macromolecule #34: Small ribosomal subunit protein RACK1
+Macromolecule #35: Small ribosomal subunit protein eS25
+Macromolecule #36: Small ribosomal subunit protein eS30
+Macromolecule #37: Small ribosomal subunit protein eS32
-Experimental details
-Structure determination
| Method | cryo EM |
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Processing | single particle reconstruction |
| Aggregation state | particle |
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Sample preparation
| Buffer | pH: 7.4 Details: 20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP |
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| Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 70 sec. / Pretreatment - Atmosphere: OTHER |
| Vitrification | Cryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 289 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
| Microscope | FEI TALOS ARCTICA |
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| Temperature | Min: 77.0 K / Max: 83.0 K |
| Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Dimensions - Width: 3838 pixel / Digitization - Dimensions - Height: 3710 pixel / Number grids imaged: 6 / Number real images: 22549 / Average electron dose: 55.0 e/Å2 |
| Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | C2 aperture diameter: 70.0 µm / Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.75 µm / Nominal defocus min: 1.25 µm / Nominal magnification: 36000 |
| Sample stage | Specimen holder model: OTHER / Cooling holder cryogen: NITROGEN |
| Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
| Initial model |
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| Output model | ![]() PDB-9uzl: |
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About Yorodumi



Keywords
Encephalomyocarditis virus
Authors
India, 1 items
Citation










Z (Sec.)
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FIELD EMISSION GUN



