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Open data
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Basic information
| Entry | ![]() | |||||||||
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| Title | PSI trimer of Arthrospira platensis NIES-39 | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Photosystem I / ELECTRON TRANSPORT / PHOTOSYNTHESIS | |||||||||
| Biological species | Arthrospira platensis NIES-39 (bacteria) | |||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 2.17 Å | |||||||||
Authors | Kato K / Nakajima Y / Shen JR / Nagao R | |||||||||
| Funding support | 1 items
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Citation | Journal: To Be PublishedTitle: Structural basis for unique low-energy chlorophylls within photosystem I in Arthrospira platensis Authors: Kato K / Nakajima Y / Shen JR / Nagao R | |||||||||
| History |
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Structure visualization
| Supplemental images |
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Downloads & links
-EMDB archive
| Map data | emd_62693.map.gz | 32.3 MB | EMDB map data format | |
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| Header (meta data) | emd-62693-v30.xml emd-62693.xml | 34.5 KB 34.5 KB | Display Display | EMDB header |
| FSC (resolution estimation) | emd_62693_fsc.xml | 13.8 KB | Display | FSC data file |
| Images | emd_62693.png | 143.3 KB | ||
| Filedesc metadata | emd-62693.cif.gz | 8.4 KB | ||
| Others | emd_62693_additional_1.map.gz emd_62693_half_map_1.map.gz emd_62693_half_map_2.map.gz | 87.8 MB 89.7 MB 89.7 MB | ||
| Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-62693 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-62693 | HTTPS FTP |
-Related structure data
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Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Map
| File | Download / File: emd_62693.map.gz / Format: CCP4 / Size: 98.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 0.752 Å | ||||||||||||||||||||||||||||||||||||
| Density |
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| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML:
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-Supplemental data
-Additional map: #1
| File | emd_62693_additional_1.map | ||||||||||||
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| Density Histograms |
-Half map: #1
| File | emd_62693_half_map_1.map | ||||||||||||
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| Density Histograms |
-Half map: #2
| File | emd_62693_half_map_2.map | ||||||||||||
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| Density Histograms |
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Sample components
+Entire : PSI-trimer
+Supramolecule #1: PSI-trimer
+Macromolecule #1: Photosystem I P700 chlorophyll a apoprotein A1
+Macromolecule #2: Photosystem I P700 chlorophyll a apoprotein A2
+Macromolecule #3: Photosystem I iron-sulfur center
+Macromolecule #4: Photosystem I reaction center subunit II
+Macromolecule #5: Photosystem I reaction center subunit IV
+Macromolecule #6: Photosystem I reaction center subunit III
+Macromolecule #7: Photosystem I reaction center subunit VIII
+Macromolecule #8: Photosystem I reaction center subunit IX
+Macromolecule #9: Unknown
+Macromolecule #10: Photosystem I reaction center subunit XI
+Macromolecule #11: Photosystem I reaction center subunit XII
+Macromolecule #12: Photosystem I reaction center subunit psaX
+Macromolecule #13: CHLOROPHYLL A ISOMER
+Macromolecule #14: CHLOROPHYLL A
+Macromolecule #15: PHYLLOQUINONE
+Macromolecule #16: IRON/SULFUR CLUSTER
+Macromolecule #17: BETA-CAROTENE
+Macromolecule #18: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+Macromolecule #19: UNKNOWN LIGAND
+Macromolecule #20: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+Macromolecule #21: CALCIUM ION
+Macromolecule #22: water
-Experimental details
-Structure determination
| Method | cryo EM |
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Processing | single particle reconstruction |
| Aggregation state | particle |
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Sample preparation
| Concentration | 2.5 mg/mL | |||||||||
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| Buffer | pH: 6.5 Component:
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| Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 10 sec. | |||||||||
| Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 281 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
| Microscope | JEOL CRYO ARM 300 |
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| Image recording | Film or detector model: GATAN K3 (6k x 4k) / Detector mode: COUNTING / Average electron dose: 49.0 e/Å2 |
| Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.6 µm / Nominal defocus min: 1.4000000000000001 µm / Nominal magnification: 60000 |
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Image processing
-Atomic model buiding 1
| Initial model | Chain - Source name: Other / Chain - Initial model type: experimental model / Details: ModelAngelo |
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| Refinement | Space: RECIPROCAL / Protocol: FLEXIBLE FIT |
| Output model | ![]() PDB-9l0a: |
Movie
Controller
About Yorodumi




Keywords
Arthrospira platensis NIES-39 (bacteria)
Authors
Citation
Z (Sec.)
Y (Row.)
X (Col.)





















































FIELD EMISSION GUN

