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- EMDB-62645: PltBd1 homopentameric holotoxin from E. coli -

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Basic information

Entry
Database: EMDB / ID: EMD-62645
TitlePltBd1 homopentameric holotoxin from E. coli
Map data
Sample
  • Complex: Recombinant Diarizonae toxin (DT) homopentamer - PltA and PltBd1 complex
    • Protein or peptide: Pertussis toxin-like subunit ArtA
    • Protein or peptide: Subtilase cytotoxin subunit B
Keywordstoxin / heteropentamer
Function / homologyBordetella pertussis toxin A / Pertussis toxin, subunit 1 / Enterotoxin / NAD+ poly-ADP-ribosyltransferase activity / extracellular region / Pertussis toxin-like subunit ArtA / Subtilase cytotoxin subunit B
Function and homology information
Biological speciesSalmonella enterica subsp. diarizonae (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.05 Å
AuthorsChen Z / Wang DD / Gao X
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Functional Synergy of Heteropentameric B Subunits Underlies Virulence in a Salmonella A2B5 Toxin
Authors: Wang D / Chen Z / Xu C / Jiao X / Yue M / Gao X
History
DepositionDec 8, 2024-
Header (metadata) releaseOct 29, 2025-
Map releaseOct 29, 2025-
UpdateOct 29, 2025-
Current statusOct 29, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_62645.png

Downloads & links

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Map

FileDownload / File: emd_62645.map.gz / Format: CCP4 / Size: 22.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.04 Å/pix.
x 180 pix.
= 187.2 Å		1.04 Å/pix.
x 180 pix.
= 187.2 Å		1.04 Å/pix.
x 180 pix.
= 187.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.04 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.35
Minimum - Maximum-3.95656 - 4.9375567
Average (Standard dev.)0.00044043773 (±0.13825658)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions180180180
Spacing180180180
CellA=B=C: 187.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_62645_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_62645_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Recombinant Diarizonae toxin (DT) homopentamer - PltA and PltBd1 ...

EntireName: Recombinant Diarizonae toxin (DT) homopentamer - PltA and PltBd1 complex
Components
  • Complex: Recombinant Diarizonae toxin (DT) homopentamer - PltA and PltBd1 complex
    • Protein or peptide: Pertussis toxin-like subunit ArtA
    • Protein or peptide: Subtilase cytotoxin subunit B

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Supramolecule #1: Recombinant Diarizonae toxin (DT) homopentamer - PltA and PltBd1 ...

SupramoleculeName: Recombinant Diarizonae toxin (DT) homopentamer - PltA and PltBd1 complex
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Salmonella enterica subsp. diarizonae (bacteria)

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Macromolecule #1: Pertussis toxin-like subunit ArtA

MacromoleculeName: Pertussis toxin-like subunit ArtA / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Salmonella enterica subsp. diarizonae (bacteria)
Molecular weightTheoretical: 27.29782 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MKKLILLTLI IASFDIYAID FVYRVDPNPP DVIFRDGFSL LGYNRDLQQL ISGRSCAGGS SDSRYIVTTS DINKTYAIAR AYYSHSKFK GNLYRYKIRA DNNFYSLTPS VNYLESQGGH FNAYEKSMIR LQSEYVSTLS ILPENIQKAV ALVYDSSTGQ I KDGTSTIN ...String:
MKKLILLTLI IASFDIYAID FVYRVDPNPP DVIFRDGFSL LGYNRDLQQL ISGRSCAGGS SDSRYIVTTS DINKTYAIAR AYYSHSKFK GNLYRYKIRA DNNFYSLTPS VNYLESQGGH FNAYEKSMIR LQSEYVSTLS ILPENIQKAV ALVYDSSTGQ I KDGTSTIN TDYVSISSVS NPGVIPFLPE PQANTQQRID AFGSLISSCF SIYSVCQTHR GQKTEVYKMP FYDARPVIQF II SGN

UniProtKB: Pertussis toxin-like subunit ArtA

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Macromolecule #2: Subtilase cytotoxin subunit B

MacromoleculeName: Subtilase cytotoxin subunit B / type: protein_or_peptide / ID: 2 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Salmonella enterica subsp. diarizonae (bacteria)
Molecular weightTheoretical: 16.576629 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MKKITSVCAL LSLICSFNAS AEWTGDYENI GYFSHEVISE FHVGQIDGGA YFCIKAVKAD GSRSTPLIAC SVSNESVWAP SFKVLLEQA RYFYVTEQSV RIYYDHNVWT NQPFVNTFST NALVGLSSCS AATDCFGPGK PKSKSKRDVE Q

UniProtKB: Subtilase cytotoxin subunit B

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation state3D array

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Sample preparation

BufferpH: 7.8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.05 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 196577
Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER

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