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- EMDB-61800: Cryo-EM structure of mGlu2-mGlu4 heterodimer bound mGlu4 agonist E7P -

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Basic information

Entry
Database: EMDB / ID: EMD-61800
TitleCryo-EM structure of mGlu2-mGlu4 heterodimer bound mGlu4 agonist E7P
Map data
Sample
  • Complex: mGlu2-mGlu4 heterodimer bound L-AP4
KeywordsGPCR / MEMBRANE PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.68 Å
AuthorsZhang Y / Liu J
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: Structural basis of allosteric activation in a metabotropic glutamate heterodimer
Authors: Zhang Y / Liu J
History
DepositionOct 4, 2024-
Header (metadata) releaseNov 27, 2024-
Map releaseNov 27, 2024-
UpdateNov 27, 2024-
Current statusNov 27, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_61800.png

Downloads & links

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Map

FileDownload / File: emd_61800.map.gz / Format: CCP4 / Size: 274.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 416 pix.
= 386.88 Å		0.93 Å/pix.
x 416 pix.
= 386.88 Å		0.93 Å/pix.
x 416 pix.
= 386.88 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.93 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.202
Minimum - Maximum-0.49167594 - 1.0535716
Average (Standard dev.)-0.00063265976 (±0.021545315)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions416416416
Spacing416416416
CellA=B=C: 386.88 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_61800_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_61800_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : mGlu2-mGlu4 heterodimer bound L-AP4

EntireName: mGlu2-mGlu4 heterodimer bound L-AP4
Components
  • Complex: mGlu2-mGlu4 heterodimer bound L-AP4

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Supramolecule #1: mGlu2-mGlu4 heterodimer bound L-AP4

SupramoleculeName: mGlu2-mGlu4 heterodimer bound L-AP4 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 52.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.6 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.68 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 112924
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: MAXIMUM LIKELIHOOD

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