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- EMDB-60026: Cryo-EM structure of an intermediate-state PSII-PRF2' complex dur... -
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Basic information
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Title | Cryo-EM structure of an intermediate-state PSII-PRF2' complex during the process of photosystem II repair | ||||||||||||
![]() | The cryo-EM map of the PSII-PRF2' complex functioning in the PSII-repair cycle. | ||||||||||||
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![]() | PHOTOSYNTHESIS | ||||||||||||
Function / homology | ![]() oxygen evolving activity / photosystem II stabilization / photosystem II / photosystem II reaction center / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / chlorophyll binding / photosynthesis, light reaction ...oxygen evolving activity / photosystem II stabilization / photosystem II / photosystem II reaction center / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / chlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / phosphate ion binding / chloroplast thylakoid membrane / photosynthetic electron transport in photosystem II / photosynthesis / electron transfer activity / protein stabilization / iron ion binding / heme binding / metal ion binding Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.9 Å | ||||||||||||
![]() | Li A / Liu Z | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for an early stage of the photosystem II repair cycle in Chlamydomonas reinhardtii. Authors: Anjie Li / Tingting You / Xiaojie Pang / Yidi Wang / Lijin Tian / Xiaobo Li / Zhenfeng Liu / ![]() Abstract: Photosystem II (PSII) catalyzes water oxidation and plastoquinone reduction by utilizing light energy. It is highly susceptible to photodamage under high-light conditions and the damaged PSII needs ...Photosystem II (PSII) catalyzes water oxidation and plastoquinone reduction by utilizing light energy. It is highly susceptible to photodamage under high-light conditions and the damaged PSII needs to be restored through a process known as the PSII repair cycle. The detailed molecular mechanism underlying the PSII repair process remains mostly elusive. Here, we report biochemical and structural features of a PSII-repair intermediate complex, likely arrested at an early stage of the PSII repair process in the green alga Chlamydomonas reinhardtii. The complex contains three protein factors associated with a damaged PSII core, namely Thylakoid Enriched Factor 14 (TEF14), Photosystem II Repair Factor 1 (PRF1), and Photosystem II Repair Factor 2 (PRF2). TEF14, PRF1 and PRF2 may facilitate the release of the manganese-stabilizing protein PsbO, disassembly of peripheral light-harvesting complexes from PSII and blockage of the Q site, respectively. Moreover, an α-tocopherol quinone molecule is located adjacent to the heme group of cytochrome b, potentially fulfilling a photoprotective role by preventing the generation of reactive oxygen species. | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 31.3 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 33.4 KB 33.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 7.4 KB | Display | ![]() |
Images | ![]() | 130.3 KB | ||
Filedesc metadata | ![]() | 8.1 KB | ||
Others | ![]() ![]() | 26.5 MB 26.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8zeeMC ![]() 8kdeC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | The cryo-EM map of the PSII-PRF2' complex functioning in the PSII-repair cycle. | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.35 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: The half cryo-EM map of the PSII-PRF2' complex...
File | emd_60026_half_map_1.map | ||||||||||||
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Annotation | The half cryo-EM map of the PSII-PRF2' complex functioning in the PSII-repair cycle. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: The half cryo-EM map of the PSII-PRF2' complex...
File | emd_60026_half_map_2.map | ||||||||||||
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Annotation | The half cryo-EM map of the PSII-PRF2' complex functioning in the PSII-repair cycle. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Cryo-EM map of the PSII-PRF2' complex functioning in the PSII-rep...
+Supramolecule #1: Cryo-EM map of the PSII-PRF2' complex functioning in the PSII-rep...
+Macromolecule #1: Photosystem II CP47 reaction center protein
+Macromolecule #2: Photosystem II D2 protein
+Macromolecule #3: Cytochrome b559 subunit alpha
+Macromolecule #4: Cytochrome b559 subunit beta
+Macromolecule #5: Photosystem II reaction center protein H
+Macromolecule #6: Photosystem II reaction center protein I
+Macromolecule #7: Photosystem II reaction center protein K
+Macromolecule #8: Photosystem II reaction center protein L
+Macromolecule #9: Photosystem II reaction center protein M
+Macromolecule #10: Photosystem II reaction center protein T
+Macromolecule #11: Photosystem II reaction center protein Psb30
+Macromolecule #12: 4.1 kDa photosystem II subunit
+Macromolecule #13: Photosystem II reaction center protein Z
+Macromolecule #14: Photosystem II CP43 reaction center protein
+Macromolecule #15: Photosystem II protein D1
+Macromolecule #16: Chains: 1
+Macromolecule #17: CHLOROPHYLL A
+Macromolecule #18: BETA-CAROTENE
+Macromolecule #19: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+Macromolecule #20: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+Macromolecule #21: BICARBONATE ION
+Macromolecule #22: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+Macromolecule #23: PHEOPHYTIN A
+Macromolecule #24: PROTOPORPHYRIN IX CONTAINING FE
+Macromolecule #25: RRR-ALPHA-TOCOPHERYLQUINONE
+Macromolecule #26: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+Macromolecule #27: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+Macromolecule #28: FE (II) ION
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 6.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |