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- EMDB-59747: Connexin 26 from Lepidosiren paradoxa solublised and purified in ... -

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Basic information

Entry
Database: EMDB / ID: EMD-59747
TitleConnexin 26 from Lepidosiren paradoxa solublised and purified in LMNG detergent
Map dataPostprocessed map with no sharpening
Sample
  • Complex: Dodecameric gap junction channel for Connexin 26
    • Protein or peptide: Connexin 26 from Lepidosiren paradoxa
KeywordsConnexin / Gap Junction Channel / Ion Channel / MEMBRANE PROTEIN
Biological speciesLepidosiren paradoxa (South American lungfish)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.51 Å
AuthorsBrotherton DH / Cameron AD
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MR/P010393/1 United Kingdom
CitationJournal: To Be Published
Title: Structure of Connexin 26 from L Paradoxa
Authors: Brotherton DH / Dale N / Cameron AD
History
DepositionSep 8, 2026-
Header (metadata) releaseSep 30, 2026-
Map releaseSep 30, 2026-
UpdateSep 30, 2026-
Current statusSep 30, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_59747.png

Downloads & links

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Map

FileDownload / File: emd_59747.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPostprocessed map with no sharpening
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.17 Å/pix.
x 200 pix.
= 233.8 Å		1.17 Å/pix.
x 200 pix.
= 233.8 Å		1.17 Å/pix.
x 200 pix.
= 233.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.169 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0106
Minimum - Maximum-0.019708574 - 0.061684746
Average (Standard dev.)0.00033018633 (±0.003016024)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 233.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half map

Fileemd_59747_half_map_1.map
AnnotationHalf map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map

Fileemd_59747_half_map_2.map
AnnotationHalf map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Dodecameric gap junction channel for Connexin 26

EntireName: Dodecameric gap junction channel for Connexin 26
Components
  • Complex: Dodecameric gap junction channel for Connexin 26
    • Protein or peptide: Connexin 26 from Lepidosiren paradoxa

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Supramolecule #1: Dodecameric gap junction channel for Connexin 26

SupramoleculeName: Dodecameric gap junction channel for Connexin 26 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Lepidosiren paradoxa (South American lungfish)

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Macromolecule #1: Connexin 26 from Lepidosiren paradoxa

MacromoleculeName: Connexin 26 from Lepidosiren paradoxa / type: protein_or_peptide / ID: 1
Details: Connexin 26 from Lepidosiren paradoxa and remainder of thrombin cutting site
Enantiomer: LEVO
Source (natural)Organism: Lepidosiren paradoxa (South American lungfish)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MNWGTLQAFL GGVNKHSTSI GKIWLSVIFI FRVMILVVAA ERVWGDEQSD FVCNTLQPGC KNVCYDHYFP IS HIRLWCL QLIFVSTPAL LVVMHVAYRR HEDKRAVLHQ DGELAEKSLQ QIKTQKVRIE GALWWTYVTS IFFRL LFEG AFMFAFYYIY NGFQMTRLVK ...String:
MNWGTLQAFL GGVNKHSTSI GKIWLSVIFI FRVMILVVAA ERVWGDEQSD FVCNTLQPGC KNVCYDHYFP IS HIRLWCL QLIFVSTPAL LVVMHVAYRR HEDKRAVLHQ DGELAEKSLQ QIKTQKVRIE GALWWTYVTS IFFRL LFEG AFMFAFYYIY NGFQMTRLVK CDAWPCPNTV DCFISRPTEK TMFTIFMIVA SVVCILLNVS ELCYLVIK A CLRHGKQEKY SSSQSSLMSK GKEHQQNKMN EFLLTNQALV PRLVPR

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
GridModel: UltrAuFoil R0./1 / Material: GOLD / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK II

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number real images: 5052 / Average exposure time: 2.0 sec. / Average electron dose: 1.032 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.3000000000000003 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: D6 (2x6 fold dihedral) / Resolution.type: BY AUTHOR / Resolution: 2.51 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 5) / Number images used: 236440
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 5)
FSC plot (resolution estimation)

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