- EMDB-5739: Electron microscopy map of the T6SS TssK component -
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基本情報
登録情報
データベース: EMDB / ID: EMD-5739
タイトル
Electron microscopy map of the T6SS TssK component
マップデータ
EM Reconstruction of TssK
試料
試料: Purified TssK sample
タンパク質・ペプチド: Type VI secretion system protein TssK
キーワード
TssK / Type VI secretion / Hcp / sheath assembly / EM / single-particle / three-fold
機能・相同性
cellular_component / biological_process / molecular_function / Type VI secretion system TssK / Type VI secretion system TssK / Bacterial Type VI secretion, VC_A0110, EvfL, ImpJ, VasE / Type VI secretion system protein TssK
ジャーナル: J Biol Chem / 年: 2013 タイトル: TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the type VI secretion system. 著者: Abdelrahim Zoued / Eric Durand / Cecilia Bebeacua / Yannick R Brunet / Badreddine Douzi / Christian Cambillau / Eric Cascales / Laure Journet / 要旨: The Type VI secretion system (T6SS) is a macromolecular machine that mediates bacteria-host or bacteria-bacteria interactions. The T6SS core apparatus assembles from 13 proteins that form two sub- ...The Type VI secretion system (T6SS) is a macromolecular machine that mediates bacteria-host or bacteria-bacteria interactions. The T6SS core apparatus assembles from 13 proteins that form two sub-assemblies: a phage-like complex and a trans-envelope complex. The Hcp, VgrG, TssE, and TssB/C subunits are structurally and functionally related to components of the tail of contractile bacteriophages. This phage-like structure is thought to be anchored to the membrane by a trans-envelope complex composed of the TssJ, TssL, and TssM proteins. However, how the two sub-complexes are connected remains unknown. Here we identify TssK, a protein that establishes contacts with the two T6SS sub-complexes through direct interactions with TssL, Hcp, and TssC. TssK is a cytoplasmic protein assembling trimers that display a three-armed shape, as revealed by TEM and SAXS analyses. Fluorescence microscopy experiments further demonstrate the requirement of TssK for sheath assembly. Our results suggest a central role for TssK by linking both complexes during T6SS assembly.
UniProtKB: Type VI secretion system protein TssK GO: biological_process, molecular_function, cellular_component InterPro: Type VI secretion system TssK
タイプ: NEGATIVE 詳細: Purified protein was immobilized on a glow- discharged carbon grid by incubation for 1 minute, and then stained with 2% uranyl acetate for 10 seconds.
グリッド
詳細: 300 mesh copper grid with thin carbon support, glow discharged for 20 seconds